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  • 93
    R&D Systems recombinant mouse il 18 il 1f4 protein
    Recombinant Mouse Il 18 Il 1f4 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse il 18 il 1f4 protein/product/R&D Systems
    Average 93 stars, based on 1 article reviews
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    recombinant mouse il 18 il 1f4 protein - by Bioz Stars, 2022-09
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    92
    LKT Laboratories bafilomycin a1
    AJ-5 reduces autophagic flux in RD and RH30 cells. a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM <t>bafilomycin</t> A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p
    Bafilomycin A1, supplied by LKT Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bafilomycin a1/product/LKT Laboratories
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bafilomycin a1 - by Bioz Stars, 2022-09
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    93
    Elabscience biotin labeling kit
    AJ-5 reduces autophagic flux in RD and RH30 cells. a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM <t>bafilomycin</t> A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p
    Biotin Labeling Kit, supplied by Elabscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin labeling kit/product/Elabscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin labeling kit - by Bioz Stars, 2022-09
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    80
    Veeco atomic force microscope
    AJ-5 reduces autophagic flux in RD and RH30 cells. a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM <t>bafilomycin</t> A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p
    Atomic Force Microscope, supplied by Veeco, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atomic force microscope/product/Veeco
    Average 80 stars, based on 1 article reviews
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    atomic force microscope - by Bioz Stars, 2022-09
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    Image Search Results


    AJ-5 reduces autophagic flux in RD and RH30 cells. a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p

    Journal: Cell Death Discovery

    Article Title: The palladacycle complex AJ-5 induces apoptotic cell death while reducing autophagic flux in rhabdomyosarcoma cells

    doi: 10.1038/s41420-019-0139-9

    Figure Lengend Snippet: AJ-5 reduces autophagic flux in RD and RH30 cells. a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p

    Article Snippet: Bafilomycin A1 disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion.

    Techniques: Western Blot, Expressing, Fluorescence