Journal:

Article Title: Galectin-7 in the Control of Epidermal Homeostasis after Injury

doi: 10.1091/mbc.E08-02-0166

Figure Lengend Snippet: Expression of differentiation and junctional markers in wt and null mutant adult epidermis. Cryosections (5 μm) of anterior foot pads from adult wt and galectin-7 −/− mice were stained. (A) Differentiation markers: keratin5 (K5)

Article Snippet: Primary antibodies were as follows: anti-galectin-7 rabbit Ab (1:3000; ), anti-Ki67 rabbit Ab (1:250, NCL-Ki67p; Novocastra, New Castle, United Kingdom), anti-keratin5 rabbit Ab (1:2500, PR-B160-P; Covance Research Products, Princeton, NJ), anti-keratin10 mouse monoclonal antibody (mAb) (1:2000, C-7284; Sigma-Aldrich), anti-loricrin rabbit Ab (1:200, PRB-145P; BAbCO, Richmond, CA), anti-E-cadherin mouse mAb (1:500, C20820; BD Biosciences, San Jose, CA), anti-β-catenin mouse mAb (1:500, C19220; BD Biosciences), anti-α6-integrin rat mAb (1:50, MAB1378; Millipore, St-Quentin-en-Yvelines, France), anti-desmoglein mouse mAb (1:10, 03-61002; American Research Products, Belmont, MA), anti-desmocollin1 rabbit Ab (1:2000, NB600-666; Novus Biologicals, Littleton, CO), anti-plakoglobin rabbit Ab (1:25, C2069-60A; U.S.

Techniques: Expressing, Mutagenesis, Mouse Assay, Staining

Journal: Disease Models & Mechanisms

Article Title: The familial dysautonomia disease gene IKBKAP is required in the developing and adult mouse central nervous system

doi: 10.1242/dmm.028258

Figure Lengend Snippet: Neuromuscular junctions in two different muscle groups are denervated in the absence of Ikbkap , and motor neuron numbers are reduced in the CKO spinal cord. Adult (4 month) anterior tibialis muscles (A-E) and erector spinae muscles (F-J) were immunolabeled with α-bungarotoxin (red) to identify cholinergic receptors, and with SV2and 2H3 (green) to label synaptic endings and axons (neurofilaments), respectively. In both muscle types, there was a significant reduction in NMJ innervation, with many terminals being partially innervated (B,H) or completely denervated (D,H). In contrast, at 1 month, neuromuscular junctions are innervated properly in both CKO mice (J) and littermate controls (I). Error bars denote s.e.m. and P- values correspond to an unpaired Student's t -test (* P

Article Snippet: Tissue was fixed for 20 min in 4% paraformaldehyde at room temperature, washed and then incubated for 15 min in α-bungarotoxin (Molecular Probes, #B160; 1 μg/ml) at room temperature, followed by PBS washes, and a 10 min incubation in −20°C methanol.

Techniques: Immunolabeling, Mouse Assay