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    Immunohistochemical markers of lymphatics and other cells in mouse lungs
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    Immunohistochemical markers of lymphatics and other cells in mouse lungs

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Imaging Lymphatics in Mouse Lungs

    doi: 10.1007/978-1-4939-8712-2_11

    Figure Lengend Snippet: Immunohistochemical markers of lymphatics and other cells in mouse lungs

    Article Snippet: Blood vessel endothelial cells Pecam1 (CD31) Armenian hamster Rat Thermo BioLegend MA3105 102502 Clone 2H8 Clone MEC13.3 Weaker expression also on lymphatic EC Endothelial cells VEGFR-2 Goat R&D Systems AF644 Weaker expression also on lymphatic EC High endothelial venules (HEV) Rat (IgM) BD Bioscience 553863 Found in bronchoalveolar lymphoid tissue (BALT) Smooth muscle cells α-smooth muscle actin Mouse Sigma Sigma F3777 (FITC) C6198 (Cy3) Marks large airways and blood vessels Pericytes NG2 Rabbit Millipore Ab5320 Epithelial cells (pan) E-cadherin Pan- Cytokeratin Rat Rabbit Thermo Dako 13–1900 Z0622 Clone ECCD-2 Clara/Club cells CCSP Rabbit Millipore 07–623 Ciliated cells Acetylated tubulin Mouse Sigma T7451 Clone 6–11B-1 Alveolar Type 1 cells Aquaporin-5 Rabbit Abcam Ab78486 Alveolar Type 2 cells SPC Rabbit Seven Hills WRAB-09337 Leukocytes (pan) CD45 Rat BioLegend 103102 Clone 30-F11 T-lymphocytes CD3e Armenian hamster BioLegend 100302 Clone 145–2C11 B-lymphocytes B220 Rat BioLegend 103202 Clone RA3–6B2 Neutrophils S100A8 A100A9 Goat Rabbit R&D Systems Abcam AF3059 Ab105472 Macrophages Iba1 F4/80 Rabbit Rat WAKO BioLegend 019–19741 123102 Also known as Aif1 Clone BM8 Better in wax sections Dendritic cells CD11c Rat eBioscience 14–0114-81 Clone N418 Better in wax sections Fibroblasts S100A4 Rabbit Abcam Ab27957 Mesothelial cells Mesothelin Goat Santa Cruz Sc-27702 Open in a separate window Immunohistochemical markers of lymphatics and other cells in mouse lungs Primary antibodies, including fluorophore-conjugated secondary antibodies (Jackson Immunoresearch), are used at a working concentration of 1 μg/mL (1:100–1:500 dilution) ( see Note 2 ).

    Techniques: Immunohistochemical staining, Marker, Expressing