Journal: PLoS Biology
Article Title: TRIM2, a novel member of the antiviral family, limits New World arenavirus entry
Figure Lengend Snippet: Role for SHP-2 in infection and TRIM2 in inhibition of phagocytosis. (A) U2OS cells were transfected with the indicated siRNAs and infected with Candid 1. Knockdowns of the RNAs are shown in S7B Fig . (B) Extracts from the brains of uninfected and Candid 1–infected mice were prepared and immunoprecipitated with anti-phosphotyrosine or anti-TRIM2 antisera and analyzed by WB with the indicated antibodies. (C) BMDMs isolated from 3 mice of each genotype were incubated with apoptotic phrodo Red–labeled thymocytes. Shown is the average percent internalization in CD11b+ cells for 3 experiments, normalized to WT in each experiment. Statistical significance was determined by unpaired t test. ** P ≤ 0.005. Representative FACS plots are in S8 Fig . No difference was seen when BMDMs from either genotype were incubated with live thymocytes ( S8 Fig ). BMDM, bone marrow–derived macrophage; FACS, fluorescence-activated cell sorting; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IP, immunoprecipitation; JUNV, Junín virus; NEFL, neurofilament light chain; NP, nucleoprotein; Phospho-ERK1/2, phosphorylated extracellular regulated kinase 1/2; siRNA, small interfering RNA; SIRPA, signal regulatory protein α; WB, western blot; WT, wild type.
Article Snippet: NEFL, SIRPA, MYO5A, SHP-2, and phospho-ERK1/2 were detected with rabbit polyclonal antibodies (CST).
Techniques: Infection, Inhibition, Transfection, Mouse Assay, Immunoprecipitation, Western Blot, Isolation, Incubation, Labeling, FACS, Derivative Assay, Fluorescence, Small Interfering RNA