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    Cell Signaling Technology Inc akt
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    Cell Signaling Technology Inc β actin
    DC-34 silencs MYC expression in cancer cells. a Inhibition of L363 MM cell proliferation at 24 (IC 50 = 3.4 µM) (red), 48 (IC 50 = 3.4 µM) (green) and 72 h (IC 50 = 3.1 µM) (blue). b Inhibition of MYC protein translation with 5 μM of DC-34 is sustained over time in L363 cells. c The half maximal inhibitory concentration (IC 50 ) of DC-34 with respect to MYC protein inhibition as determined by Peggy protein expression. d A Western blot of MYC protein during a representative cycloheximide-chase degradation experiment with L363 multiple myeloma cells. Cells were treated with 10 µg/mL of cycloheximide (CX) in the absence or presence of DC-34 (5 µM) and MYC protein expression was assessed at indicated time points. <t>β-actin</t> was used as loading control. e MYC protein levels are inhibited as a function of the dose of DC-34 in L363 cells; only the highest dose of DC-34 affected MYC in the more resistant CA46 Burkitt’s lymphoma cells. f Western blot analysis of 293T cells transiently transfected with either GFP or CMV-MYC plasmid (the CMV promoter lacks a MYC G4) (right) and dosed with different concentrations of DC-34. All western blots were exposed for
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    Cell Signaling Technology Inc caspase 3
    DC-34 silencs MYC expression in cancer cells. a Inhibition of L363 MM cell proliferation at 24 (IC 50 = 3.4 µM) (red), 48 (IC 50 = 3.4 µM) (green) and 72 h (IC 50 = 3.1 µM) (blue). b Inhibition of MYC protein translation with 5 μM of DC-34 is sustained over time in L363 cells. c The half maximal inhibitory concentration (IC 50 ) of DC-34 with respect to MYC protein inhibition as determined by Peggy protein expression. d A Western blot of MYC protein during a representative cycloheximide-chase degradation experiment with L363 multiple myeloma cells. Cells were treated with 10 µg/mL of cycloheximide (CX) in the absence or presence of DC-34 (5 µM) and MYC protein expression was assessed at indicated time points. <t>β-actin</t> was used as loading control. e MYC protein levels are inhibited as a function of the dose of DC-34 in L363 cells; only the highest dose of DC-34 affected MYC in the more resistant CA46 Burkitt’s lymphoma cells. f Western blot analysis of 293T cells transiently transfected with either GFP or CMV-MYC plasmid (the CMV promoter lacks a MYC G4) (right) and dosed with different concentrations of DC-34. All western blots were exposed for
    Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DC-34 silencs MYC expression in cancer cells. a Inhibition of L363 MM cell proliferation at 24 (IC 50 = 3.4 µM) (red), 48 (IC 50 = 3.4 µM) (green) and 72 h (IC 50 = 3.1 µM) (blue). b Inhibition of MYC protein translation with 5 μM of DC-34 is sustained over time in L363 cells. c The half maximal inhibitory concentration (IC 50 ) of DC-34 with respect to MYC protein inhibition as determined by Peggy protein expression. d A Western blot of MYC protein during a representative cycloheximide-chase degradation experiment with L363 multiple myeloma cells. Cells were treated with 10 µg/mL of cycloheximide (CX) in the absence or presence of DC-34 (5 µM) and MYC protein expression was assessed at indicated time points. β-actin was used as loading control. e MYC protein levels are inhibited as a function of the dose of DC-34 in L363 cells; only the highest dose of DC-34 affected MYC in the more resistant CA46 Burkitt’s lymphoma cells. f Western blot analysis of 293T cells transiently transfected with either GFP or CMV-MYC plasmid (the CMV promoter lacks a MYC G4) (right) and dosed with different concentrations of DC-34. All western blots were exposed for

    Journal: Nature Communications

    Article Title: Chemical and structural studies provide a mechanistic basis for recognition of the MYC G-quadruplex

    doi: 10.1038/s41467-018-06315-w

    Figure Lengend Snippet: DC-34 silencs MYC expression in cancer cells. a Inhibition of L363 MM cell proliferation at 24 (IC 50 = 3.4 µM) (red), 48 (IC 50 = 3.4 µM) (green) and 72 h (IC 50 = 3.1 µM) (blue). b Inhibition of MYC protein translation with 5 μM of DC-34 is sustained over time in L363 cells. c The half maximal inhibitory concentration (IC 50 ) of DC-34 with respect to MYC protein inhibition as determined by Peggy protein expression. d A Western blot of MYC protein during a representative cycloheximide-chase degradation experiment with L363 multiple myeloma cells. Cells were treated with 10 µg/mL of cycloheximide (CX) in the absence or presence of DC-34 (5 µM) and MYC protein expression was assessed at indicated time points. β-actin was used as loading control. e MYC protein levels are inhibited as a function of the dose of DC-34 in L363 cells; only the highest dose of DC-34 affected MYC in the more resistant CA46 Burkitt’s lymphoma cells. f Western blot analysis of 293T cells transiently transfected with either GFP or CMV-MYC plasmid (the CMV promoter lacks a MYC G4) (right) and dosed with different concentrations of DC-34. All western blots were exposed for

    Article Snippet: Proteins were separated by SDS-PAGE and analyzed by immunoblotting with c-myc (ab32072) and β-actin (cell signaling #4967) primary antibodies.

    Techniques: Expressing, Inhibition, Concentration Assay, Western Blot, Transfection, Plasmid Preparation