Journal: Journal of hypertension
Article Title: (Pro)renin receptor contributes to regulation of renal epithelial sodium channel
Figure Lengend Snippet: Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC
Article Snippet: Antibodies to PRR (1 : 1000 dilutions, Abcam, Cambridge, Massachusetts, USA), prorenin/renin (1 : 200 dilutions; Santa Cruz biotechnology, Inc., Santa Cruz, California, USA), SGK-1 (1 : 1000 dilutions, Cell Signaling, USA), p-Nedd4–2 (1 : 1000 dilutions, Abcam, Cambridge, Massachusetts, USA), α-ENaC (1 : 500 dilutions; ASC-030, Alomone Labs, Israel) were used in the Western blot as previously described [ , ].
Techniques: Expressing, shRNA