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    Alomone Labs α enac
    Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla <t>α-ENaC</t>
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    Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC

    Journal: Journal of hypertension

    Article Title: (Pro)renin receptor contributes to regulation of renal epithelial sodium channel

    doi: 10.1097/HJH.0000000000000825

    Figure Lengend Snippet: Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC

    Article Snippet: Antibodies to PRR (1 : 1000 dilutions, Abcam, Cambridge, Massachusetts, USA), prorenin/renin (1 : 200 dilutions; Santa Cruz biotechnology, Inc., Santa Cruz, California, USA), SGK-1 (1 : 1000 dilutions, Cell Signaling, USA), p-Nedd4–2 (1 : 1000 dilutions, Abcam, Cambridge, Massachusetts, USA), α-ENaC (1 : 500 dilutions; ASC-030, Alomone Labs, Israel) were used in the Western blot as previously described [ , ].

    Techniques: Expressing, shRNA

    Role of ENaCα subunit in flow-stimulated Na + currents A. Representative Western blot (left panel), and cumulative data (middle) demonstrating ENaCα protein levels in cells transfected with non-targeting siRNA (scramble), and cells transfected with ENaCα siRNA. β-actin used as loading control (* p

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Regulation of mechanosensitive biliary epithelial transport by the Epithelial Na+ Channel, ENaC

    doi: 10.1002/hep.28301

    Figure Lengend Snippet: Role of ENaCα subunit in flow-stimulated Na + currents A. Representative Western blot (left panel), and cumulative data (middle) demonstrating ENaCα protein levels in cells transfected with non-targeting siRNA (scramble), and cells transfected with ENaCα siRNA. β-actin used as loading control (* p

    Article Snippet: Localization of ENaCα protein was performed in confluent MLC monolayers utilizing anti-ENaCα antibody (Alomone labs) and then Dylight 488 conjugated donkey anti-rabbit antibody (Jackson, 1:600) and counter-labeled with DAPI and Alexa Fluor 555 phalloidin.

    Techniques: Flow Cytometry, Western Blot, Transfection

    Functional effects of overexpression of ENaC subunits on flow-stimulated Na + currents A. Representative Western blot (left panel) and cumulative data (right panel) demonstrating relative change in ENaCα protein level in control Mz-Cha-1 cells, cells transfected with non-targeting siRNA (mock), ENaCα siRNA, ENaC α subunit (overexpression), and GFP alone. β-actin used as loading control. *p

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Regulation of mechanosensitive biliary epithelial transport by the Epithelial Na+ Channel, ENaC

    doi: 10.1002/hep.28301

    Figure Lengend Snippet: Functional effects of overexpression of ENaC subunits on flow-stimulated Na + currents A. Representative Western blot (left panel) and cumulative data (right panel) demonstrating relative change in ENaCα protein level in control Mz-Cha-1 cells, cells transfected with non-targeting siRNA (mock), ENaCα siRNA, ENaC α subunit (overexpression), and GFP alone. β-actin used as loading control. *p

    Article Snippet: Localization of ENaCα protein was performed in confluent MLC monolayers utilizing anti-ENaCα antibody (Alomone labs) and then Dylight 488 conjugated donkey anti-rabbit antibody (Jackson, 1:600) and counter-labeled with DAPI and Alexa Fluor 555 phalloidin.

    Techniques: Functional Assay, Over Expression, Flow Cytometry, Western Blot, Transfection

    Expression and localization of ENaC in biliary epithelium A. RT-PCR. Species specific ENaC subunit primers were used to detect ENaC subunits in all models. In human biliary Mz-Cha-1 cells, ENaC α, β, and γ were detected (band sizes of 349, 349, and 288 respectively). In mouse large cholangiocytes (MLC, left panel) and mouse small cholangiocytes (MSC, right panel) ENaCα and γ (band sizes of 424 and 427, respectively) were detected. B. Membrane localization of ENaCα protein in polarized MLC monolayers. Staining with Alexa Fluor 555 phalloidin (red), to label the cell membrane, anti-ENaCα antibody (green), and DAPI (blue), demonstrates ENaCα protein in the apical plasma membrane, right panel (x-z plane shown below each image, arrow head indicates apical membrane). Control preparations (without primary antibody), right panel. Scale bar =10μm. C. Localization of ENaCα in mouse whole liver sections. Left panel, ENaCα was expressed on the apical membrane of intrahepatic bile ducts (arrow) and hepatic artery (arrow head). Middle panel, ENaCα is also detected in hepatic sinusoids lined by endothelial cells, while the portal vein (pv) and central vein (cv), have weaker expression. Right panel, the gallbladder shows strong staining in both the apical surface of the epithelial cells as well as macrophages in the lamina propria (arrow). All images at 400x magnification.

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Regulation of mechanosensitive biliary epithelial transport by the Epithelial Na+ Channel, ENaC

    doi: 10.1002/hep.28301

    Figure Lengend Snippet: Expression and localization of ENaC in biliary epithelium A. RT-PCR. Species specific ENaC subunit primers were used to detect ENaC subunits in all models. In human biliary Mz-Cha-1 cells, ENaC α, β, and γ were detected (band sizes of 349, 349, and 288 respectively). In mouse large cholangiocytes (MLC, left panel) and mouse small cholangiocytes (MSC, right panel) ENaCα and γ (band sizes of 424 and 427, respectively) were detected. B. Membrane localization of ENaCα protein in polarized MLC monolayers. Staining with Alexa Fluor 555 phalloidin (red), to label the cell membrane, anti-ENaCα antibody (green), and DAPI (blue), demonstrates ENaCα protein in the apical plasma membrane, right panel (x-z plane shown below each image, arrow head indicates apical membrane). Control preparations (without primary antibody), right panel. Scale bar =10μm. C. Localization of ENaCα in mouse whole liver sections. Left panel, ENaCα was expressed on the apical membrane of intrahepatic bile ducts (arrow) and hepatic artery (arrow head). Middle panel, ENaCα is also detected in hepatic sinusoids lined by endothelial cells, while the portal vein (pv) and central vein (cv), have weaker expression. Right panel, the gallbladder shows strong staining in both the apical surface of the epithelial cells as well as macrophages in the lamina propria (arrow). All images at 400x magnification.

    Article Snippet: Localization of ENaCα protein was performed in confluent MLC monolayers utilizing anti-ENaCα antibody (Alomone labs) and then Dylight 488 conjugated donkey anti-rabbit antibody (Jackson, 1:600) and counter-labeled with DAPI and Alexa Fluor 555 phalloidin.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining