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    Alomone Labs guinea pig anti asic1
    Marker expression for T7 DRG neurons in the absence of Merkel cells
    Guinea Pig Anti Asic1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    guinea pig anti asic1 - by Bioz Stars, 2023-06
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    Marker expression for T7 DRG neurons in the absence of Merkel cells

    Journal: The Journal of Neuroscience

    Article Title: Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes

    doi: 10.1523/JNEUROSCI.3781-15.2016

    Figure Lengend Snippet: Marker expression for T7 DRG neurons in the absence of Merkel cells

    Article Snippet: The primary antibodies used were mouse anti-Islet1/2 (1:50; catalog #39.4D5, Developmental Studies Hybridoma Bank), rabbit anti-NF200 (1:1000; N4142, Sigma-Aldrich), goat anti-TrkB (1:200; AF1494, R&D Systems), goat anti-TrkC [AF1404 (1:100) and BAF1404 (1:20), R&D Systems], rabbit anti-Ret (1:50; catalog #18121, Immuno-Biological Laboratories), rabbit anti-parvalbumin (PV; 1:1000; PV25, Swant), rabbit anti-CGRP (1:1000; T-4032, Peninsula Laboratories), rabbit anti-phospho-SMAD1/5/8 (1:250; catalog 9511, Cell Signaling Technology), goat anti-TBX3 (1:100; sc-31656, Santa Cruz Biotechnology), rat anti-Keratin8 (1:20, TROMA-1, Developmental Studies Hybridoma Bank), guinea pig anti-Asic1 (1:250; Alomone Labs), rabbit anti-δ opioid receptor (DOR; 1:500; Alomone Labs), and rabbit anti-γENaC (1:500; StressMarq Biosciences).

    Techniques: Marker, Expressing

    Merkel cell deletion alters the expression of SAI ion channel components. A, qPCR analysis of ion channels in P21 thoracic DRGs (n ≥ 8 mice/genotype at each age). B–G, Immunostaining for NF200, γENaC, Asic1, and DOR in T7 DRG sections from P21 control littermate and K14; Atoh1CKO mice. Asterisks indicate double-positive cells. H, Percentages of Islet1/2+ T7 DRG neurons that were NF200+ γENaC+, NF200+Asic1, and NF200+DOR+ in P21 control and K14; Atoh1CKO mice (n ≥ 3 mice/genotype). Error bars in graphs represent the SEM, and asterisks indicate statistically significant differences between genotypes. *p < 0.05, ***p < 0.001.

    Journal: The Journal of Neuroscience

    Article Title: Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes

    doi: 10.1523/JNEUROSCI.3781-15.2016

    Figure Lengend Snippet: Merkel cell deletion alters the expression of SAI ion channel components. A, qPCR analysis of ion channels in P21 thoracic DRGs (n ≥ 8 mice/genotype at each age). B–G, Immunostaining for NF200, γENaC, Asic1, and DOR in T7 DRG sections from P21 control littermate and K14; Atoh1CKO mice. Asterisks indicate double-positive cells. H, Percentages of Islet1/2+ T7 DRG neurons that were NF200+ γENaC+, NF200+Asic1, and NF200+DOR+ in P21 control and K14; Atoh1CKO mice (n ≥ 3 mice/genotype). Error bars in graphs represent the SEM, and asterisks indicate statistically significant differences between genotypes. *p < 0.05, ***p < 0.001.

    Article Snippet: The primary antibodies used were mouse anti-Islet1/2 (1:50; catalog #39.4D5, Developmental Studies Hybridoma Bank), rabbit anti-NF200 (1:1000; N4142, Sigma-Aldrich), goat anti-TrkB (1:200; AF1494, R&D Systems), goat anti-TrkC [AF1404 (1:100) and BAF1404 (1:20), R&D Systems], rabbit anti-Ret (1:50; catalog #18121, Immuno-Biological Laboratories), rabbit anti-parvalbumin (PV; 1:1000; PV25, Swant), rabbit anti-CGRP (1:1000; T-4032, Peninsula Laboratories), rabbit anti-phospho-SMAD1/5/8 (1:250; catalog 9511, Cell Signaling Technology), goat anti-TBX3 (1:100; sc-31656, Santa Cruz Biotechnology), rat anti-Keratin8 (1:20, TROMA-1, Developmental Studies Hybridoma Bank), guinea pig anti-Asic1 (1:250; Alomone Labs), rabbit anti-δ opioid receptor (DOR; 1:500; Alomone Labs), and rabbit anti-γENaC (1:500; StressMarq Biosciences).

    Techniques: Expressing, Immunostaining