Journal: The Journal of Neuroscience
Article Title: Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes
Figure Lengend Snippet: Merkel cell deletion alters the expression of SAI ion channel components. A , qPCR analysis of ion channels in P21 thoracic DRGs ( n ≥ 8 mice/genotype at each age). B–G , Immunostaining for NF200, γENaC, Asic1, and DOR in T7 DRG sections from P21 control littermate and K14; Atoh1 CKO mice. Asterisks indicate double-positive cells. H , Percentages of Islet1/2 + T7 DRG neurons that were NF200 + γENaC + , NF200 + Asic1, and NF200 + DOR + in P21 control and K14; Atoh1 CKO mice ( n ≥ 3 mice/genotype). Error bars in graphs represent the SEM, and asterisks indicate statistically significant differences between genotypes. * p
Article Snippet: The primary antibodies used were mouse anti-Islet1/2 (1:50; catalog #39.4D5, Developmental Studies Hybridoma Bank), rabbit anti-NF200 (1:1000; N4142, Sigma-Aldrich), goat anti-TrkB (1:200; AF1494, R & D Systems), goat anti-TrkC [AF1404 (1:100) and BAF1404 (1:20), R & D Systems], rabbit anti-Ret (1:50; catalog #18121, Immuno-Biological Laboratories), rabbit anti-parvalbumin (PV; 1:1000; PV25, Swant), rabbit anti-CGRP (1:1000; T-4032, Peninsula Laboratories), rabbit anti-phospho-SMAD1/5/8 (1:250; catalog 9511, Cell Signaling Technology), goat anti-TBX3 (1:100; sc-31656, Santa Cruz Biotechnology), rat anti-Keratin8 (1:20, TROMA-1, Developmental Studies Hybridoma Bank), guinea pig anti-Asic1 (1:250; Alomone Labs), rabbit anti-δ opioid receptor (DOR; 1:500; Alomone Labs), and rabbit anti-γENaC (1:500; StressMarq Biosciences).
Techniques: Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Immunostaining