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    Alomone Labs asic2a
    Parkin suppresses PICK1-mediated potentiation of <t>ASIC2a</t> currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
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    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Journal:

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    doi: 10.1091/mbc.E05-11-1027

    Figure Lengend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Article Snippet: Further, PICK1 is believed to target activated PKCα to ASIC2a, thereby leading to the phosphorylation of the channel in its cytoplasmic tail and to the potentiation of ASIC2a currents ( ).

    Techniques:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Journal:

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    doi: 10.1091/mbc.E05-11-1027

    Figure Lengend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Article Snippet: Further, PICK1 is believed to target activated PKCα to ASIC2a, thereby leading to the phosphorylation of the channel in its cytoplasmic tail and to the potentiation of ASIC2a currents ( ).

    Techniques: Knock-Out, Mouse Assay

    ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults

    doi:

    Figure Lengend Snippet: ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Article Snippet: Cerebral cortical neurons were collected and fixed with 4% paraformaldehyde in PBS for 30 min. Then permeabilized with PBS/0.3% Triton X-100 for 30 min, and all cells were blocked with 2% goat serum and 1% bovine serum albumin (BSA) in PBS for 1 h, and then incubated with anti-ASIC1 (Alomone labs, Israel) or anti-ASIC2a (Alomone labs, Israel) overnight at 4°C.

    Techniques: Expressing, Immunofluorescence, Western Blot