Journal: International Journal of Clinical and Experimental Pathology
Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults
Figure Lengend Snippet: ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P
Article Snippet: Cerebral cortical neurons were collected and fixed with 4% paraformaldehyde in PBS for 30 min. Then permeabilized with PBS/0.3% Triton X-100 for 30 min, and all cells were blocked with 2% goat serum and 1% bovine serum albumin (BSA) in PBS for 1 h, and then incubated with anti-ASIC1 (Alomone labs, Israel) or anti-ASIC2a (Alomone labs, Israel) overnight at 4°C.
Techniques: Expressing, Immunofluorescence, Western Blot