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    • p2x 7  (Alomone Labs)
    88
    Alomone Labs p2x 7
    <t>P2X</t> <t>7</t> receptor expression and ATP release of MNL. ( a – c ) show unchallenged MNL examined by FACS and ( d and e ) show leukotoxin-exposed MNL. ( a ) Population identification (R1 and R2) of MNL based on FCS/SSC characteristics. A representative dot-plot is shown. ( b ) Distribution of cells with different surface markers in the two gated populations (R1 and R2). Mean values±S.D. from three different MNL donors are shown. ( c ) Illustration of P2X 7 R expression in R1 and R2 by representative histograms. ( d ) Number of viable cells (PI-negative) in the two different MNL populations (R1 and R2) analyzed by FACS during leukotoxin exposure (10 ng/ml) with and without oATP (500 μ M). ( e ) ATP-release from the macrophages exposed to the leukotoxin (1 and 10 ng/ml)
    P2x 7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x 7/product/Alomone Labs
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    p2x 7 - by Bioz Stars, 2023-02
    88/100 stars
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    P2X 7 receptor expression and ATP release of MNL. ( a – c ) show unchallenged MNL examined by FACS and ( d and e ) show leukotoxin-exposed MNL. ( a ) Population identification (R1 and R2) of MNL based on FCS/SSC characteristics. A representative dot-plot is shown. ( b ) Distribution of cells with different surface markers in the two gated populations (R1 and R2). Mean values±S.D. from three different MNL donors are shown. ( c ) Illustration of P2X 7 R expression in R1 and R2 by representative histograms. ( d ) Number of viable cells (PI-negative) in the two different MNL populations (R1 and R2) analyzed by FACS during leukotoxin exposure (10 ng/ml) with and without oATP (500 μ M). ( e ) ATP-release from the macrophages exposed to the leukotoxin (1 and 10 ng/ml)

    Journal: Cell Death & Disease

    Article Title: Cellular and molecular response of human macrophages exposed to Aggregatibacter actinomycetemcomitans leukotoxin

    doi: 10.1038/cddis.2011.6

    Figure Lengend Snippet: P2X 7 receptor expression and ATP release of MNL. ( a – c ) show unchallenged MNL examined by FACS and ( d and e ) show leukotoxin-exposed MNL. ( a ) Population identification (R1 and R2) of MNL based on FCS/SSC characteristics. A representative dot-plot is shown. ( b ) Distribution of cells with different surface markers in the two gated populations (R1 and R2). Mean values±S.D. from three different MNL donors are shown. ( c ) Illustration of P2X 7 R expression in R1 and R2 by representative histograms. ( d ) Number of viable cells (PI-negative) in the two different MNL populations (R1 and R2) analyzed by FACS during leukotoxin exposure (10 ng/ml) with and without oATP (500 μ M). ( e ) ATP-release from the macrophages exposed to the leukotoxin (1 and 10 ng/ml)

    Article Snippet: The two distinct populations (G1 and G2) were characterized with specific fluorescein (FITC)-conjugated antibodies for expression of cell surface receptors: CD14 (Clone TüK4), CD3 (Clone UCHT1) CD19 (Clone HD37) (all from DakoCytomation, Copenhagen, Denmark) and P2X 7 (Rabbit polyclonal) (Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing