Journal: European journal of immunology
Article Title: Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Figure Lengend Snippet: ]. Samples were taken and stained with a 0.24 DAPI solution and measured using a 355 nm Genesis CX laser (100 mW, Coherent, Santa Clara, CA, USA, MoFlo Legacy cell sorter, (Beckman Coulter, Brea, California, USA). Scatter was measured using a 488 nm Sapphire OPS laser (400 mW). Beads of sizes 0.5 μm and 1 μm Fluoresbrite Microspheres (both Polysciences, 18339 and 17458, Warrington, PA, USA) were amended into every sample as internal standards. A master gate (D and G: grey) was defined that comprised 200 000 cells for each measurement. Each upcoming subcommunity was marked by a gate in the three samples and a combined gate template generated (D and G: black ellipses). The three chosen samples show the highly diverse cytometric structure of the community and its evolution over time.
Article Snippet: Magnetic-bead enrichment: Following CD1d-PBS57-APC tetramer staining, iNKT cells may be enriched using anti-APC magnetic microbeads following the manufacturer's instructions (Miltenyi Biotec).
Techniques: Staining, Generated