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    Miltenyi Biotec anti apc microbeads
    Flow cytometric analysis of cell states in pure cultures of Escherichia coli K12 grown on complex DSM 381 medium. Cells were sampled, treated with 2% PFA and fixed in 70% ice-cold ethanol. Following, cells were stained with a 0.24 μM DAPI solution and measured using a 355 nm Genesis CX laser (100 mW, both Coherent, Santa Clara, CA). Scatter was measured using a 488 nm Sapphire OPS laser (400 mW). 0.5 μm and 1 μm Fluoresbrite <t>Microspheres</t> (both Polysciences, 18339 and 17458, Warrington, PA, USA) were added to every sample as internal standards. A and C show nearly identical proliferation patterns during lag and stationary phases of growth. B shows the proliferation pattern (uncoupled DNA synthesis) in the log-phase. The right graph D shows the growth curve where samples were taken from 500 ml batch cultures with 200 ml medium for a time range of 24 hours. After a short lag phase the cells immediately started exponential growth which ended after about 20 hours.
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    Flow cytometric analysis of cell states in pure cultures of Escherichia coli K12 grown on complex DSM 381 medium. Cells were sampled, treated with 2% PFA and fixed in 70% ice-cold ethanol. Following, cells were stained with a 0.24 μM DAPI solution and measured using a 355 nm Genesis CX laser (100 mW, both Coherent, Santa Clara, CA). Scatter was measured using a 488 nm Sapphire OPS laser (400 mW). 0.5 μm and 1 μm Fluoresbrite Microspheres (both Polysciences, 18339 and 17458, Warrington, PA, USA) were added to every sample as internal standards. A and C show nearly identical proliferation patterns during lag and stationary phases of growth. B shows the proliferation pattern (uncoupled DNA synthesis) in the log-phase. The right graph D shows the growth curve where samples were taken from 500 ml batch cultures with 200 ml medium for a time range of 24 hours. After a short lag phase the cells immediately started exponential growth which ended after about 20 hours.

    Journal: European journal of immunology

    Article Title: Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

    doi: 10.1002/eji.201970107

    Figure Lengend Snippet: Flow cytometric analysis of cell states in pure cultures of Escherichia coli K12 grown on complex DSM 381 medium. Cells were sampled, treated with 2% PFA and fixed in 70% ice-cold ethanol. Following, cells were stained with a 0.24 μM DAPI solution and measured using a 355 nm Genesis CX laser (100 mW, both Coherent, Santa Clara, CA). Scatter was measured using a 488 nm Sapphire OPS laser (400 mW). 0.5 μm and 1 μm Fluoresbrite Microspheres (both Polysciences, 18339 and 17458, Warrington, PA, USA) were added to every sample as internal standards. A and C show nearly identical proliferation patterns during lag and stationary phases of growth. B shows the proliferation pattern (uncoupled DNA synthesis) in the log-phase. The right graph D shows the growth curve where samples were taken from 500 ml batch cultures with 200 ml medium for a time range of 24 hours. After a short lag phase the cells immediately started exponential growth which ended after about 20 hours.

    Article Snippet: Magnetic-bead enrichment: Following CD1d-PBS57-APC tetramer staining, iNKT cells may be enriched using anti-APC magnetic microbeads following the manufacturer's instructions (Miltenyi Biotec).

    Techniques: Staining, DNA Synthesis

    ]. Samples were taken and stained with a 0.24 DAPI solution and measured using a 355 nm Genesis CX laser (100 mW, Coherent, Santa Clara, CA, USA, MoFlo Legacy cell sorter, (Beckman Coulter, Brea, California, USA). Scatter was measured using a 488 nm Sapphire OPS laser (400 mW). Beads of sizes 0.5 μm and 1 μm Fluoresbrite Microspheres (both Polysciences, 18339 and 17458, Warrington, PA, USA) were amended into every sample as internal standards. A master gate (D and G: grey) was defined that comprised 200 000 cells for each measurement. Each upcoming subcommunity was marked by a gate in the three samples and a combined gate template generated (D and G: black ellipses). The three chosen samples show the highly diverse cytometric structure of the community and its evolution over time.

    Journal: European journal of immunology

    Article Title: Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

    doi: 10.1002/eji.201970107

    Figure Lengend Snippet: ]. Samples were taken and stained with a 0.24 DAPI solution and measured using a 355 nm Genesis CX laser (100 mW, Coherent, Santa Clara, CA, USA, MoFlo Legacy cell sorter, (Beckman Coulter, Brea, California, USA). Scatter was measured using a 488 nm Sapphire OPS laser (400 mW). Beads of sizes 0.5 μm and 1 μm Fluoresbrite Microspheres (both Polysciences, 18339 and 17458, Warrington, PA, USA) were amended into every sample as internal standards. A master gate (D and G: grey) was defined that comprised 200 000 cells for each measurement. Each upcoming subcommunity was marked by a gate in the three samples and a combined gate template generated (D and G: black ellipses). The three chosen samples show the highly diverse cytometric structure of the community and its evolution over time.

    Article Snippet: Magnetic-bead enrichment: Following CD1d-PBS57-APC tetramer staining, iNKT cells may be enriched using anti-APC magnetic microbeads following the manufacturer's instructions (Miltenyi Biotec).

    Techniques: Staining, Generated