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    Alomone Labs anti kca1 1
    NKβ1 down-regulation reduces the cell surface expression of <t>Slo1</t> channels in HEK293T cells. A, Cell-surface biotinylation assay showing cell surface and total expression of Myc-Slo1 in HEK293T cell transiently cotransfected with si-NKβ1
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    NKβ1 down-regulation reduces the cell surface expression of Slo1 channels in HEK293T cells. A, Cell-surface biotinylation assay showing cell surface and total expression of Myc-Slo1 in HEK293T cell transiently cotransfected with si-NKβ1

    Journal: FEBS letters

    Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

    doi: 10.1016/j.febslet.2009.08.039

    Figure Lengend Snippet: NKβ1 down-regulation reduces the cell surface expression of Slo1 channels in HEK293T cells. A, Cell-surface biotinylation assay showing cell surface and total expression of Myc-Slo1 in HEK293T cell transiently cotransfected with si-NKβ1

    Article Snippet: Antibodies used in this work include: anti-Myc (9B11, Cell Signaling Technology, Inc); anti-Myc (06-549, Upstate Biotechnology, Inc); anti-NKβ1 (ab33144, Abcam Inc. Cambridge, MA), anti-α-Na+ /K+ -ATPase (anti-NKα1) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) and anti-Slo1 (APC-107, Alomone Laboratories, Jerusalem, Israel).

    Techniques: Expressing, Cell Surface Biotinylation Assay

    NKβ1 interacts with neuronal Slo1 channels. A, B Schematic representations of Slo1 and NKβ1 respectively. The bait fragment used in the yeast two-hybrid screen is highlighted in blue. C, Co-immunoprecipitation of NKβ1 and Slo1

    Journal: FEBS letters

    Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

    doi: 10.1016/j.febslet.2009.08.039

    Figure Lengend Snippet: NKβ1 interacts with neuronal Slo1 channels. A, B Schematic representations of Slo1 and NKβ1 respectively. The bait fragment used in the yeast two-hybrid screen is highlighted in blue. C, Co-immunoprecipitation of NKβ1 and Slo1

    Article Snippet: Antibodies used in this work include: anti-Myc (9B11, Cell Signaling Technology, Inc); anti-Myc (06-549, Upstate Biotechnology, Inc); anti-NKβ1 (ab33144, Abcam Inc. Cambridge, MA), anti-α-Na+ /K+ -ATPase (anti-NKα1) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) and anti-Slo1 (APC-107, Alomone Laboratories, Jerusalem, Israel).

    Techniques: Two Hybrid Screening, Immunoprecipitation

    Interaction between NKβ1 and Slo1 occurs at multiple regions. A, GST pull-down assay carried out on chick CG lysates showing that a fusion protein containing the bait, GST-Slo1(G785-A985) binds to NKβ1. Additional fusion proteins from

    Journal: FEBS letters

    Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

    doi: 10.1016/j.febslet.2009.08.039

    Figure Lengend Snippet: Interaction between NKβ1 and Slo1 occurs at multiple regions. A, GST pull-down assay carried out on chick CG lysates showing that a fusion protein containing the bait, GST-Slo1(G785-A985) binds to NKβ1. Additional fusion proteins from

    Article Snippet: Antibodies used in this work include: anti-Myc (9B11, Cell Signaling Technology, Inc); anti-Myc (06-549, Upstate Biotechnology, Inc); anti-NKβ1 (ab33144, Abcam Inc. Cambridge, MA), anti-α-Na+ /K+ -ATPase (anti-NKα1) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) and anti-Slo1 (APC-107, Alomone Laboratories, Jerusalem, Israel).

    Techniques: Pull Down Assay

    Co-localization pattern of NKβ1 and Slo1. Top panel shows co-localization of NKβ1 (red) and Slo1 (green) in E9 chick CG neurons. The regions enclosed by white squares are magnified in the bottom panels to show neurites and cell bodies.

    Journal: FEBS letters

    Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

    doi: 10.1016/j.febslet.2009.08.039

    Figure Lengend Snippet: Co-localization pattern of NKβ1 and Slo1. Top panel shows co-localization of NKβ1 (red) and Slo1 (green) in E9 chick CG neurons. The regions enclosed by white squares are magnified in the bottom panels to show neurites and cell bodies.

    Article Snippet: Antibodies used in this work include: anti-Myc (9B11, Cell Signaling Technology, Inc); anti-Myc (06-549, Upstate Biotechnology, Inc); anti-NKβ1 (ab33144, Abcam Inc. Cambridge, MA), anti-α-Na+ /K+ -ATPase (anti-NKα1) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) and anti-Slo1 (APC-107, Alomone Laboratories, Jerusalem, Israel).

    Techniques:

    Inside-out patch recordings made from HEK293T cells expressing Slo1. A, Typical families of currents evoked by voltage steps (-80 mV to +80 mV) in inside-out patches from transfected HEK293T cells. The bath solution contained 20 μM Ca 2+ . No currents

    Journal: FEBS letters

    Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

    doi: 10.1016/j.febslet.2009.08.039

    Figure Lengend Snippet: Inside-out patch recordings made from HEK293T cells expressing Slo1. A, Typical families of currents evoked by voltage steps (-80 mV to +80 mV) in inside-out patches from transfected HEK293T cells. The bath solution contained 20 μM Ca 2+ . No currents

    Article Snippet: Antibodies used in this work include: anti-Myc (9B11, Cell Signaling Technology, Inc); anti-Myc (06-549, Upstate Biotechnology, Inc); anti-NKβ1 (ab33144, Abcam Inc. Cambridge, MA), anti-α-Na+ /K+ -ATPase (anti-NKα1) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) and anti-Slo1 (APC-107, Alomone Laboratories, Jerusalem, Israel).

    Techniques: Expressing, Transfection

    Large-conductance Ca 2+ -activated K + channel α-subunit (BKα) and BKγ1 form molecular complex on the cell surface in mouse bronchial smooth muscle cells (mBSMCs). A : coimmunoprecipitation assay was performed using rat BSMs (rBSMs). Lysates were precipitated with anti-BKα antibody and blotted using either anti-BKα or anti-BKγ1 antibody. Similar results were obtained from 3 independent experiments. Two rats were used for each experiment. Lane 1 , lysates from rBSM; lane 2 , samples precipitated with control resin that cannot bind to antibodies; lane 3 , samples precipitated with resin that binds to anti-BKα antibody. B : total internal reflection fluorescence (TIRF) imaging of mBSMCs, in which BKα and BKγ1 were labeled with each antibody. Fluorescent signals from particles corresponding to BKα, BKγ1, and colocalization are shown in green, red, and yellow, respectively. Merged image was overlapped with a cell image. C : ratio of BKα particles localized alone or colocalized with BKγ1 to total BKα particles in mBSMCs (BKα alone, 134 particles and colocalized, 214 particles from 15 cells).

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Roles of LRRC26 as an auxiliary γ1-subunit of large-conductance Ca2+-activated K+ channels in bronchial smooth muscle cells

    doi: 10.1152/ajplung.00331.2019

    Figure Lengend Snippet: Large-conductance Ca 2+ -activated K + channel α-subunit (BKα) and BKγ1 form molecular complex on the cell surface in mouse bronchial smooth muscle cells (mBSMCs). A : coimmunoprecipitation assay was performed using rat BSMs (rBSMs). Lysates were precipitated with anti-BKα antibody and blotted using either anti-BKα or anti-BKγ1 antibody. Similar results were obtained from 3 independent experiments. Two rats were used for each experiment. Lane 1 , lysates from rBSM; lane 2 , samples precipitated with control resin that cannot bind to antibodies; lane 3 , samples precipitated with resin that binds to anti-BKα antibody. B : total internal reflection fluorescence (TIRF) imaging of mBSMCs, in which BKα and BKγ1 were labeled with each antibody. Fluorescent signals from particles corresponding to BKα, BKγ1, and colocalization are shown in green, red, and yellow, respectively. Merged image was overlapped with a cell image. C : ratio of BKα particles localized alone or colocalized with BKγ1 to total BKα particles in mBSMCs (BKα alone, 134 particles and colocalized, 214 particles from 15 cells).

    Article Snippet: The blots were incubated with anti-LRRC26 antibody (1:100 dilution, sc-132325; Santa Cruz Biotechnology, Houston, TX), anti-BKα antibody (1:200 dilution, APC-107, Alomone Laboratories), or anti-BKβ1 antibody (1:100 dilution, APC-036, Alomone Laboratories) and then incubated with horseradish peroxidase-conjugated IgG antibody (Chemicon International, Temecula, CA).

    Techniques: Co-Immunoprecipitation Assay, Fluorescence, Imaging, Labeling

    Large-conductance Ca 2+ -activated K + channel γ1-subunit (BKγ1) expression is selectively high in mouse bronchial smooth muscle (BSM). A : real-time PCR analyses of BKγ subunits (BKγ1–4) in mouse bronchial smooth muscles (BSMs; N = 3). B : real-time PCR analyses of BKα, BKβ1, and BKγ1 in several types of mouse SM tissues ( N = 4–5). C : real-time PCR analysis was performed to compare BKγ1 mRNA expression between mouse trachea and bronchus (trachea, N = 3; bronchus, N = 5). * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Roles of LRRC26 as an auxiliary γ1-subunit of large-conductance Ca2+-activated K+ channels in bronchial smooth muscle cells

    doi: 10.1152/ajplung.00331.2019

    Figure Lengend Snippet: Large-conductance Ca 2+ -activated K + channel γ1-subunit (BKγ1) expression is selectively high in mouse bronchial smooth muscle (BSM). A : real-time PCR analyses of BKγ subunits (BKγ1–4) in mouse bronchial smooth muscles (BSMs; N = 3). B : real-time PCR analyses of BKα, BKβ1, and BKγ1 in several types of mouse SM tissues ( N = 4–5). C : real-time PCR analysis was performed to compare BKγ1 mRNA expression between mouse trachea and bronchus (trachea, N = 3; bronchus, N = 5). * P

    Article Snippet: The blots were incubated with anti-LRRC26 antibody (1:100 dilution, sc-132325; Santa Cruz Biotechnology, Houston, TX), anti-BKα antibody (1:200 dilution, APC-107, Alomone Laboratories), or anti-BKβ1 antibody (1:100 dilution, APC-036, Alomone Laboratories) and then incubated with horseradish peroxidase-conjugated IgG antibody (Chemicon International, Temecula, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction