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    Alomone Labs anti kv1 3 kcna3 extracellular pe antibody
    Selective role of KCa3.1 on the migration of activated CD3 + T cells. A. ShK inhibition of native <t>Kv1.3</t> currents. Representative Kv1.3 currents were recorded in activated T cells before (control) and after <t>extracellular</t> application of 10 nM ShK. Currents were elicited by depolarizing pulses to +50 mV from a holding potential (HP) of −80 mV every 30 s. The average inhibition of Kv1.3 currents is shown in the lower panel (n = 6). B. Mean velocity before and after ShK (n = 7). The effect of the Kv1.3 inhibitor ShK (10 nM) on cell migration was determined by following single cells for 20 min before and after treatment with the blocker. C. TRAM-34 inhibition of native KCa3.1 currents. Representative KCa3.1 currents were recorded in activated T cells before (control) and after extracellular application of 250 nM TRAM-34. Currents were induced from a HP of −80 mV by a ramp depolarization from −120 mV to +40 mV every 10 s. The average KCa3.1 currents in absence and presence of TRAM-34 are shown in the lower panel (n = 9). D. Mean velocity before and after TRAM-34 (n = 22). The effect of 250 nM TRAM-34 was determined by following single cells for 20 min before and after TRAM-34 application.
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    Selective role of KCa3.1 on the migration of activated CD3 + T cells. A. ShK inhibition of native Kv1.3 currents. Representative Kv1.3 currents were recorded in activated T cells before (control) and after extracellular application of 10 nM ShK. Currents were elicited by depolarizing pulses to +50 mV from a holding potential (HP) of −80 mV every 30 s. The average inhibition of Kv1.3 currents is shown in the lower panel (n = 6). B. Mean velocity before and after ShK (n = 7). The effect of the Kv1.3 inhibitor ShK (10 nM) on cell migration was determined by following single cells for 20 min before and after treatment with the blocker. C. TRAM-34 inhibition of native KCa3.1 currents. Representative KCa3.1 currents were recorded in activated T cells before (control) and after extracellular application of 250 nM TRAM-34. Currents were induced from a HP of −80 mV by a ramp depolarization from −120 mV to +40 mV every 10 s. The average KCa3.1 currents in absence and presence of TRAM-34 are shown in the lower panel (n = 9). D. Mean velocity before and after TRAM-34 (n = 22). The effect of 250 nM TRAM-34 was determined by following single cells for 20 min before and after TRAM-34 application.

    Journal: PLoS ONE

    Article Title: KCa3.1 and TRPM7 Channels at the Uropod Regulate Migration of Activated Human T Cells

    doi: 10.1371/journal.pone.0043859

    Figure Lengend Snippet: Selective role of KCa3.1 on the migration of activated CD3 + T cells. A. ShK inhibition of native Kv1.3 currents. Representative Kv1.3 currents were recorded in activated T cells before (control) and after extracellular application of 10 nM ShK. Currents were elicited by depolarizing pulses to +50 mV from a holding potential (HP) of −80 mV every 30 s. The average inhibition of Kv1.3 currents is shown in the lower panel (n = 6). B. Mean velocity before and after ShK (n = 7). The effect of the Kv1.3 inhibitor ShK (10 nM) on cell migration was determined by following single cells for 20 min before and after treatment with the blocker. C. TRAM-34 inhibition of native KCa3.1 currents. Representative KCa3.1 currents were recorded in activated T cells before (control) and after extracellular application of 250 nM TRAM-34. Currents were induced from a HP of −80 mV by a ramp depolarization from −120 mV to +40 mV every 10 s. The average KCa3.1 currents in absence and presence of TRAM-34 are shown in the lower panel (n = 9). D. Mean velocity before and after TRAM-34 (n = 22). The effect of 250 nM TRAM-34 was determined by following single cells for 20 min before and after TRAM-34 application.

    Article Snippet: The specificity of the extracellular anti-Kv1.3 antibody has been shown by us , .

    Techniques: Migration, Inhibition

    Differential localization of Kv1.3 and KCa3.1 in migrating T cells. A. Distribution of Kv1.3 and KCa3.1 at the uropod. T cells were transiently transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CD44 antibody (uropod; red) without permeabilization. Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. B. Distribution of Kv1.3 and KCa3.1 at the leading-edge. T cells, transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CXCR-4 antibody (leading-edge; red) without permeabilization, were analyzed by confocal microscopy. Colocalization between the two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. C. Correlation coefficients for KCa3.1 and Kv1.3 localization in the uropod (U) and leading-edge (L). The data are the average of n = 15 cells for KCa3.1 at the U and n = 8 at the L, and n = 16 for Kv1.3 at the U and n = 11 at the U from 2 healthy individuals. Statistical significance was established by one way ANOVA. D. Localization of native Kv1.3 in the leading-edge. T cells from one healthy individual were fixed and stained with extracellular anti-Kv1.3 antibody (green) together with antibodies either against CD44 (red; left) or CXCR-4 (red, right). Yellow colors in the merge images indicate strong correlation. Scale bar = 5 µm. E. Average Correlation coefficients of native Kv1.3 with the leading-edge (L) (n = 9) and the uropod (U) markers (n = 11).

    Journal: PLoS ONE

    Article Title: KCa3.1 and TRPM7 Channels at the Uropod Regulate Migration of Activated Human T Cells

    doi: 10.1371/journal.pone.0043859

    Figure Lengend Snippet: Differential localization of Kv1.3 and KCa3.1 in migrating T cells. A. Distribution of Kv1.3 and KCa3.1 at the uropod. T cells were transiently transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CD44 antibody (uropod; red) without permeabilization. Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. B. Distribution of Kv1.3 and KCa3.1 at the leading-edge. T cells, transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CXCR-4 antibody (leading-edge; red) without permeabilization, were analyzed by confocal microscopy. Colocalization between the two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. C. Correlation coefficients for KCa3.1 and Kv1.3 localization in the uropod (U) and leading-edge (L). The data are the average of n = 15 cells for KCa3.1 at the U and n = 8 at the L, and n = 16 for Kv1.3 at the U and n = 11 at the U from 2 healthy individuals. Statistical significance was established by one way ANOVA. D. Localization of native Kv1.3 in the leading-edge. T cells from one healthy individual were fixed and stained with extracellular anti-Kv1.3 antibody (green) together with antibodies either against CD44 (red; left) or CXCR-4 (red, right). Yellow colors in the merge images indicate strong correlation. Scale bar = 5 µm. E. Average Correlation coefficients of native Kv1.3 with the leading-edge (L) (n = 9) and the uropod (U) markers (n = 11).

    Article Snippet: The specificity of the extracellular anti-Kv1.3 antibody has been shown by us , .

    Techniques: Transfection, Staining, Confocal Microscopy