Journal: International Journal of Molecular Sciences
Article Title: Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase
Figure Lengend Snippet: Expression of CD140α, CD44, CD34 and SK3 cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p
Article Snippet: Cells were then washed with PBS for 10 min and incubated with the following primary antibodies in a humidified chamber overnight at 4 °C: anti-CD34 (ab81289; Abcam, Cambridge, UK), anti-CD44 (BD Pharmingen, San Diego, CA, USA), anti-KCa2.3-ATTO-594 (SK3; Alomone Labs, Jerusalem, Israel), anti-PDGFRα (ab234965; Abcam, Cambridge, UK), and anti-nNOS (3G6B10; Invitrogen Carlsbad, CA, USA).
Techniques: Expressing, Flow Cytometry, Cell Counting, Fluorescence