Journal: The European Journal of Neuroscience
Article Title: Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells
Figure Lengend Snippet: Somato-dendritic distribution of the Kir3.2 subunit in CA1 PCs. (A) A low-magnification image of a CA1 PC soma with an emerging apical dendrite (Ap.Dendr) labelled for the Kir3.2 subunit (10-nm gold). (B and C) Higher magnification images of the boxed areas shown in panel A. (D) A spiny apical dendrite from the distal (dist.) part of SR is shown. (E) A higher magnification image of the boxed area shown in D. (F) A low-magnification image of a spiny tuft dendrite (Tuft Dendr.) in the SLM labelled for the Kir3.2 subunit (10 nm gold). (G) A higher magnification image of the boxed area shown in F. (H and I) Gold particles for the Kir3.2 subunit are associated with the P-faces of spiny oblique dendrites (Obl.Dendr.) in the proximal (H) and distal (I) parts of SR. (J) Bar graphs demonstrate the densities (mean ± SD) of gold particles in different subcellular compartments of CA1 PCs. *Values that are significantly different from the background labelling ( anova , P < 0.001; Dunnett's post hoc test, P < 0.01; n = 3 rats). The inset shows that gold particle density in the AIS was not significantly different from the background labelling ( anova , P < 0.001; Dunnett's post hoc test, P = 0.95; n = 3 rats). The AISs were identified based on their immunopositivity for pan-NF. BG, background; mid., middle; Sp, spine; AxTerm, axon terminal. Scale bars, 1 μm (A), 500 nm (D and F), 250 nm (H and I), 100 nm (B, C, E and G).
Article Snippet: Kir3.2 , Rabbit , aa. 374–414 of mouse Kir3.2 , 4 , 1.3 , Alomone Labs , APC-006 , Koyrakh et al . , Kulik et al . ( ) .