Journal: The European Journal of Neuroscience

Article Title: Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells

doi: 10.1111/ejn.12526

Figure Lengend Snippet: Antibodies

Article Snippet: Kir3.2 , Rabbit , aa. 374–414 of mouse Kir3.2 , 4 , 1.3 , Alomone Labs , APC-006 , Koyrakh et al . , Kulik et al . ( ) .

Techniques:

Journal: The European Journal of Neuroscience

Article Title: Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells

doi: 10.1111/ejn.12526

Figure Lengend Snippet: Densities of gold particles labelling three K + channel subunits in distinct subcellular compartments of CA1 PCs

Article Snippet: Kir3.2 , Rabbit , aa. 374–414 of mouse Kir3.2 , 4 , 1.3 , Alomone Labs , APC-006 , Koyrakh et al . , Kulik et al . ( ) .

Techniques:

Journal: The European Journal of Neuroscience

Article Title: Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells

doi: 10.1111/ejn.12526

Figure Lengend Snippet: Distributions of three different Kir3 subunits in the CA1 region of rat hippocampus. (A) Immunofluorescent reaction for the Kir3.1 subunit shows a modest labelling in SO and in the proximal part of SR with a gradual increase towards the distal part of the SR and SLM. (B) Similar labelling pattern is observed for the Kir3.2 subunit, with the most intense immunolabelling in the distal part of the SR and SLM. (C) The CA1 area shows weak immunoreactivity for the Kir3.3 subunit, but the intensity of the signal increases toward the distal SR and SLM. Scale bars, 100 μm (A–C).

Article Snippet: Kir3.2 , Rabbit , aa. 374–414 of mouse Kir3.2 , 4 , 1.3 , Alomone Labs , APC-006 , Koyrakh et al . , Kulik et al . ( ) .

Techniques:

Journal: The European Journal of Neuroscience

Article Title: Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells

doi: 10.1111/ejn.12526

Figure Lengend Snippet: Somato-dendritic distribution of the Kir3.2 subunit in CA1 PCs. (A) A low-magnification image of a CA1 PC soma with an emerging apical dendrite (Ap.Dendr) labelled for the Kir3.2 subunit (10-nm gold). (B and C) Higher magnification images of the boxed areas shown in panel A. (D) A spiny apical dendrite from the distal (dist.) part of SR is shown. (E) A higher magnification image of the boxed area shown in D. (F) A low-magnification image of a spiny tuft dendrite (Tuft Dendr.) in the SLM labelled for the Kir3.2 subunit (10 nm gold). (G) A higher magnification image of the boxed area shown in F. (H and I) Gold particles for the Kir3.2 subunit are associated with the P-faces of spiny oblique dendrites (Obl.Dendr.) in the proximal (H) and distal (I) parts of SR. (J) Bar graphs demonstrate the densities (mean ± SD) of gold particles in different subcellular compartments of CA1 PCs. *Values that are significantly different from the background labelling ( anova , P < 0.001; Dunnett's post hoc test, P < 0.01; n = 3 rats). The inset shows that gold particle density in the AIS was not significantly different from the background labelling ( anova , P < 0.001; Dunnett's post hoc test, P = 0.95; n = 3 rats). The AISs were identified based on their immunopositivity for pan-NF. BG, background; mid., middle; Sp, spine; AxTerm, axon terminal. Scale bars, 1 μm (A), 500 nm (D and F), 250 nm (H and I), 100 nm (B, C, E and G).

Article Snippet: Kir3.2 , Rabbit , aa. 374–414 of mouse Kir3.2 , 4 , 1.3 , Alomone Labs , APC-006 , Koyrakh et al . , Kulik et al . ( ) .

Techniques: