Journal: Frontiers in Neuroscience
Article Title: Anti-Nogo-A Immunotherapy Does Not Alter Hippocampal Neurogenesis after Stroke in Adult Rats
Figure Lengend Snippet: Nogo-A is expressed by immature neurons in the adult dentate gyrus. (A) Nogo-A is expressed in the processes and somata of immature neurons (arrowheads), which are also positive for doublecortin (DCX). Scale bar: 25 μm. (B) Horizontally-oriented DCX+/Nogo-A+ neuroblast. Scale bar: 25 μm. (C) Nogo-A expression is not appreciable in NeuN+ mature granule cells, the majority of NeuN+ cells in the GCL. However, putative basket cells (arrow) label strongly for Nogo-A. Scale bar: 25 μm. (D) Nogo-A immunoreactivity is not detectable in GFAP+ stem cells or astrocytes. Scale bar: 50 μm. (E) S1PR2 is broadly expressed in the GCL, including DCX+ immature neurons and NeuN+ mature neurons. Scale bar: 20 μm. (F) S1PR2 expression by GFAP+/Sox2+ neural stem cells in the SGZ. Scale bar: 25 μm. (G) Lack of NgR1 expression by DCX+ immature neurons. Scale bars: 25 μm; 10 μm (inset). (H) Lack of NgR1 expression by GFAP+ neural stem cells in the SGZ. Scale bar: 25 μm. ML, molecular layer; GCL, granule cell layer; PL, polymorphic layer.
Article Snippet: Rabbit anti-NgR1 , Alomone Labs ANT-008 [ RRID: AB_2040180 ] , 1:250.