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    Alomone Labs anti chrm1 antibody
    Muscarinic 1 receptor (M1) activation regulates the activity of qNSCs in SVZ niche. A . Immunofluorescence staining for VCAM 1 (Purple) and <t>ChRM1</t> (green) in the SVZ niche from P30 C57BL/6J mice. B . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) from the SVZ NSCs culture. C . Western blot detection of pEGFR (PhosphoY1068) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures in proliferation media collected after 12 hours. D . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel C. *P = 0.0107, n=4, Unpaired t-test. E . Immunofluorescence staining for EdU (purple) and Ki67 (green) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in proliferation media collected after one day. F . Analysis of normalized quantification of Ki67 + cells per well in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel E. P = n.s., n=5, Unpaired t-test. G . Analysis of normalized quantification of EdU + /Ki67 + cells ratio per well in control and treated samples with carbachol (15μM) of SVZ NSCs cultures from panel E. **P = 0.0012, n=6, Unpaired t-test. H . Western blot detection of pEGFR(PhosphoY1068)/β-actin in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in differentiation media collected every 12 hours for 36 hours. I . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures from panel H. P
    Anti Chrm1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti chrm1 antibody - by Bioz Stars, 2022-08
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    Muscarinic 1 receptor (M1) activation regulates the activity of qNSCs in SVZ niche. A . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) in the SVZ niche from P30 C57BL/6J mice. B . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) from the SVZ NSCs culture. C . Western blot detection of pEGFR (PhosphoY1068) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures in proliferation media collected after 12 hours. D . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel C. *P = 0.0107, n=4, Unpaired t-test. E . Immunofluorescence staining for EdU (purple) and Ki67 (green) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in proliferation media collected after one day. F . Analysis of normalized quantification of Ki67 + cells per well in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel E. P = n.s., n=5, Unpaired t-test. G . Analysis of normalized quantification of EdU + /Ki67 + cells ratio per well in control and treated samples with carbachol (15μM) of SVZ NSCs cultures from panel E. **P = 0.0012, n=6, Unpaired t-test. H . Western blot detection of pEGFR(PhosphoY1068)/β-actin in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in differentiation media collected every 12 hours for 36 hours. I . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures from panel H. P

    Journal: bioRxiv

    Article Title: Uncovering a neural circuit controlling adult quiescent neural stem cell activation in the subventricular zone

    doi: 10.1101/2021.12.27.474262

    Figure Lengend Snippet: Muscarinic 1 receptor (M1) activation regulates the activity of qNSCs in SVZ niche. A . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) in the SVZ niche from P30 C57BL/6J mice. B . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) from the SVZ NSCs culture. C . Western blot detection of pEGFR (PhosphoY1068) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures in proliferation media collected after 12 hours. D . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel C. *P = 0.0107, n=4, Unpaired t-test. E . Immunofluorescence staining for EdU (purple) and Ki67 (green) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in proliferation media collected after one day. F . Analysis of normalized quantification of Ki67 + cells per well in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel E. P = n.s., n=5, Unpaired t-test. G . Analysis of normalized quantification of EdU + /Ki67 + cells ratio per well in control and treated samples with carbachol (15μM) of SVZ NSCs cultures from panel E. **P = 0.0012, n=6, Unpaired t-test. H . Western blot detection of pEGFR(PhosphoY1068)/β-actin in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in differentiation media collected every 12 hours for 36 hours. I . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures from panel H. P

    Article Snippet: We used primary antibodies to GFP (#GFP-1020, 1:400, AVES lab), Choline Acetyltransferase Antibody (#AB144P, 1:250, Millipore sigma), tdTomato [16D7] (#EST203, 1:200, Kerafast), RFP (#600-401-379, 1:250, Rockland), Calretinin (#ab702, 1:200, Abcam), calretinin (#6B3, 1:250, Swant), Calretinin (#MCA-3G9, 1:250, EnCor Biotechnology), RFP (#ab62341, 1:200, Abcam), Doublecortin (#AB2253, 1:250, Millipore), Doublecortin (#4604S, 1:200, Cell Signaling Technology), Ki67 (ab15580, 1:250: Abcam), Ki67 (#CPCA-Ki67, 1:200, EnCor Biotechnology), beta Actin HRP (#MA515739HRP (BA3R), 1:5000, Thermo Fisher Scientific), HRP-conjugated Beta Actin (#HRP-60008, 1:5000, Proteintech), EGFR (phospho Y1068) (#ab5644, 1:250, Abcam), EGFR (phospho Y1068) [Y38] (#ab32430, 1:250, Abcam), CD106 (#550547, 1:100, BD Diagnostic Systems), GFAP (#GFAP, 1:500, Aves Labs), M1 Muscarinic Receptor (#AMR-001, Alomone Labs).

    Techniques: Activation Assay, Activity Assay, Immunofluorescence, Staining, Mouse Assay, Western Blot