Article Title: miRNA-mediated downregulation of KCC2 and VGAT expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats
Figure Lengend Snippet: Dual in situ hybridization and immunohistochemistry in spinal cord (L6-S1) demonstrate co-expression of miR-92b-3p with both presynaptic and postsynaptic GABA-associated transporters, cotransporter, receptor in spinal dorsal horn neurons. The close arrows demonstrate co-expression of miR-92b-3p (green) with KCC2 in fig. 6D and with GABA Aα-1 in fig. 6H (red). The VGAT staining that are not colocalized but closely apposed to miR-92b-3p expression are shown in fig. 6L (open arrows). The scale bars for A, E I are 50 µm, for B, C, D, F, G H are 10 µm, for J, K L are 5 µm.
Article Snippet: After completing the treatment with anti-digoxigenin-fluorescence (1:500, Roche) for 2hr at room temperature, spinal cord tissue sections were incubated overnight with either one of the following antibodies at 1:100 dilution, rabbit anti-KCC2 (Millipore), rabbit anti-VGAT (Alomone Labs) and rabbit anti-GABAA-α1 (Alomone Labs).
Techniques: In Situ Hybridization, Immunohistochemistry, Expressing, Staining