Journal: Cell reports
Article Title: CPEB3-dependent increase in GluA2 subunits impairs excitatory transmission onto inhibitory interneurons in a mouse model of fragile X
Figure Lengend Snippet: (A and B) Upper: representative images of individual endogenous GluA1 (red) (A) or GluA2 (red) (B) mRNA molecules within a single hippocampal neuron detected by single-molecule FISH, followed by immunofluorescence with antibodies directed to the presynaptic marker bassoon (green), the dendritic marker MAP2 (blue), and merge. Lower: higher-magnification images of boxed region in the upper panel. (C) Upper: representative images of individual GluA1 (red) and GluA2 (green) mRNA molecules in the same neuron. Lower: higher-magnification images. (D) Summary data of images like those in (A and B) showing the number of AMPAR GluA1 (black) and GluA2 (red) mRNAs that juxtapose to synaptic sites marked by basoon (first two bars) or that colocalize with each other (third bar). GluA1 and GluA2, 30dendrites, 30 neurons, n = 4. (E) Summary data for images like those in (C) showing the number of GluA1 and GluA2 molecules per dendrite. (F) GluA1 and GluA2 mRNA molecules as a function of the distance from the soma (GluA1, 101 dendrites, 35 neurons, n = 4; GluA2, 190 dendrites, 82 neurons, n = 4). The number of individual GluA1 and GluA2 mRNA molecules in all dendrites of all hippocampal neurons that met the criteria for identification as mRNAs were analyzed by an individual blinded to the treatment. Scale bar, 10 μm. For (D–F): Data are mean ± SEM. **p < 0.01. NS, not significant. Here and in , , (with the exception of ), , and , n is defined as number of independent experiments each involving a different batch of neurons. In , n is defined as number of animals.
Article Snippet: Rabbit polyclonal anti-AMPA Receptor 1 (GluR1)/GluA1 , Alomone Labs , Cat# AGC-004; RRID:AB_2039878.
Techniques: Immunofluorescence, Marker