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    Alomone Labs anti nr2b
    NMDA channel subunit <t>NR2B</t> expression in surface, parietal, and chief cells is transcriptionally regulated in HP-infected tissues. Paraffin-embedded tissues from ( A ) sham- (Contr-20 wkPI) or ( B ) 6 and ( C ) 20 wkPI HP-infected mice were stained for the NR2B
    Anti Nr2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti nr2b - by Bioz Stars, 2022-07
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    NMDA channel subunit NR2B expression in surface, parietal, and chief cells is transcriptionally regulated in HP-infected tissues. Paraffin-embedded tissues from ( A ) sham- (Contr-20 wkPI) or ( B ) 6 and ( C ) 20 wkPI HP-infected mice were stained for the NR2B

    Journal: Gastroenterology

    Article Title: N-methyl D-Aspartate Channels Link Ammonia and Epithelial Cell Death Mechanisms in Helicobacter pylori Infection

    doi: 10.1053/j.gastro.2011.08.048

    Figure Lengend Snippet: NMDA channel subunit NR2B expression in surface, parietal, and chief cells is transcriptionally regulated in HP-infected tissues. Paraffin-embedded tissues from ( A ) sham- (Contr-20 wkPI) or ( B ) 6 and ( C ) 20 wkPI HP-infected mice were stained for the NR2B

    Article Snippet: Filters were incubated with anti-NR2B (Alomone Labs, Jerusalem, Israel), anti-BAX (BD Pharmingen, San Jose, CA), or anti-BAK (Santa Cruz, Santa Cruz, CA).

    Techniques: Expressing, Infection, Mouse Assay, Staining

    TG2 localises extracellularly in primary astrocytes and at synaptic sites in neurons. A) Immunofluorescence staining of primary astrocytes. Cells were fixed in 4% paraformaldehyde - 4 % sucrose (w/v), permeabilized (left panels) or non-permeabilized (right panel) and stained with anti-TG2 IA12 (green), DAPI (blue) and astrocytic marker anti-GFAP (red). Coverslips were visualised by laser scanning Leica SP5 confocal microscope using 63X oil immersion objective. Successive serial optical sections (1 µm) were recorded over 8 µm planes. Scale bar 20 µm. TG2 intensity was calculated by Leica software, divided by number of nuclei, and normalised to permeabilized values. Data is expressed as mean ± SD (N=3, Mann-Whitney test: p=NS). B) Neurons at 12 DIV were fixed and permeabilized (left panel) or non-permeabilized (right panel) and stained with anti-TG2 IA12 (green), anti-β-TUB or anti-NR2B (red) antibodies and DAPI (blue). Scale bar 10 µm. TG2 intensity was calculated as described in A (N=3, Mann-Whitney test: *p

    Journal: bioRxiv

    Article Title: Astrocytic extracellular vesicles modulate neuronal calcium homeostasis via transglutaminase-2

    doi: 10.1101/2021.09.30.462507

    Figure Lengend Snippet: TG2 localises extracellularly in primary astrocytes and at synaptic sites in neurons. A) Immunofluorescence staining of primary astrocytes. Cells were fixed in 4% paraformaldehyde - 4 % sucrose (w/v), permeabilized (left panels) or non-permeabilized (right panel) and stained with anti-TG2 IA12 (green), DAPI (blue) and astrocytic marker anti-GFAP (red). Coverslips were visualised by laser scanning Leica SP5 confocal microscope using 63X oil immersion objective. Successive serial optical sections (1 µm) were recorded over 8 µm planes. Scale bar 20 µm. TG2 intensity was calculated by Leica software, divided by number of nuclei, and normalised to permeabilized values. Data is expressed as mean ± SD (N=3, Mann-Whitney test: p=NS). B) Neurons at 12 DIV were fixed and permeabilized (left panel) or non-permeabilized (right panel) and stained with anti-TG2 IA12 (green), anti-β-TUB or anti-NR2B (red) antibodies and DAPI (blue). Scale bar 10 µm. TG2 intensity was calculated as described in A (N=3, Mann-Whitney test: *p

    Article Snippet: Immunocytochemical stainingCells were fixed in 4% paraformaldehyde - 4 % sucrose (w/v) and immunofluorescence staining was performed using the following antibodies: mouse monoclonal anti-TG2 (IA12 – Tim Johnson, University of Sheffield ( )), guinea pig anti-VGLUT1 (Synaptic System, Goettingen, Germany), rabbit anti-GFAP (Dako, Agilent, Santa Clara, CA, USA), rabbit anti-Shank2 (Synaptic System), rabbit anti-Fibronectin (Sigma-Aldrich), rabbit anti-β-tubulin (Sigma-Aldrich) and rabbit anti-NR2B (Alomone, Jerusalem, Israel).

    Techniques: Immunofluorescence, Staining, Marker, Microscopy, Software, MANN-WHITNEY