Journal: Scientific Reports
Article Title: Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission
Figure Lengend Snippet: Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and GABA A R α1, α2, α3 and α5 subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).
Article Snippet: Membranes were incubated in appropriate primary antibodies (anti-KCC2 1:3000 (Merck), anti-NKCC1 1:1000 (Abcam), anti-β-tubulin 1:10,000 (Neuromics), anti-human APP W02 1:2000 (Merk), total APP anti-C-terminus 1:4000 (Sigma Aldrich), anti-soluble APP clone 22C11 1:500 (Merck), anti-GABA B R 1:500 (Sigma Aldrich), anti-GABA A R α1 1:1000 (Alomone labs), anti-GABA A R α2 1:1000 (Abcam), anti-GABA A R α3 1:1000 (Alomone labs), anti-GABA A R α5 1:1000 (Alomone labs), anti-GluA1 1:500 (Merck), anti-GluA2 1:1000 (Merck), anti-GluN2A 1:250 (Merck), anti-GluN2B 1:500 (BD Biosciences), anti-GAD65/67 1:1000 (Abcam)) at 4 °C, overnight.
Techniques: Western Blot, Two Tailed Test