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    Alomone Labs rabbit anti gaba a r α3 polyclonal antibody
    Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) <t>α3</t> subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.
    Rabbit Anti Gaba A R α3 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gaba a r α3 polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gaba a r α3 polyclonal antibody - by Bioz Stars, 2022-05
    93/100 stars
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    Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) α3 subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling

    doi: 10.3389/fcimb.2019.00073

    Figure Lengend Snippet: Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) α3 subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.

    Article Snippet: To probe GABA-A subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody (Alomone labs), rabbit anti-GABA-A R α5 polyclonal antibody (Synaptic system), and mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab) ON at 4°C.

    Techniques: Immunostaining, Staining

    Modulated transcriptional expression of GABAergic and VDCC signaling components in  Toxoplasma -challenged primary microglia. The relative expression (2 −Δ Ct ) for each target gene in  Toxoplasma  (PTG)-challenged primary microglia was normalized to that of in unchallenged microglia and the differences are represented as percentage increase (red color intensity scale) or decrease (blue scale) in the heat map. For both conditions, unchallenged and  Toxoplasma -challenged, data is represented as mean of 2–4 independent experiments at indicated time points. The modulation of mRNA expression is shown for  (A)  GABA synthesis and degrading enzymes,  (B)  GABA transporters,  (C)  GABA-A R subunits,  (D)  CCCs, and  (E)  VDCCs.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling

    doi: 10.3389/fcimb.2019.00073

    Figure Lengend Snippet: Modulated transcriptional expression of GABAergic and VDCC signaling components in Toxoplasma -challenged primary microglia. The relative expression (2 −Δ Ct ) for each target gene in Toxoplasma (PTG)-challenged primary microglia was normalized to that of in unchallenged microglia and the differences are represented as percentage increase (red color intensity scale) or decrease (blue scale) in the heat map. For both conditions, unchallenged and Toxoplasma -challenged, data is represented as mean of 2–4 independent experiments at indicated time points. The modulation of mRNA expression is shown for (A) GABA synthesis and degrading enzymes, (B) GABA transporters, (C) GABA-A R subunits, (D) CCCs, and (E) VDCCs.

    Article Snippet: To probe GABA-A subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody (Alomone labs), rabbit anti-GABA-A R α5 polyclonal antibody (Synaptic system), and mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab) ON at 4°C.

    Techniques: Expressing