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2022-05
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Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling
doi: 10.3389/fcimb.2019.00073
Figure Lengend Snippet: Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) α3 subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.
Article Snippet: To probe GABA-A subunits, cells were incubated with
Techniques: Immunostaining, Staining

Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling
doi: 10.3389/fcimb.2019.00073
Figure Lengend Snippet: Modulated transcriptional expression of GABAergic and VDCC signaling components in Toxoplasma -challenged primary microglia. The relative expression (2 −Δ Ct ) for each target gene in Toxoplasma (PTG)-challenged primary microglia was normalized to that of in unchallenged microglia and the differences are represented as percentage increase (red color intensity scale) or decrease (blue scale) in the heat map. For both conditions, unchallenged and Toxoplasma -challenged, data is represented as mean of 2–4 independent experiments at indicated time points. The modulation of mRNA expression is shown for (A) GABA synthesis and degrading enzymes, (B) GABA transporters, (C) GABA-A R subunits, (D) CCCs, and (E) VDCCs.
Article Snippet: To probe GABA-A subunits, cells were incubated with
Techniques: Expressing