Journal: Molecular Cancer
Article Title: Enhanced therapeutic effect using sequential administration of antigenically distinct oncolytic viruses expressing oncostatin M in a Syrian hamster orthotopic pancreatic cancer model
Figure Lengend Snippet: Human OSM is biologically active on PANC-1 and HaP-T1 cell lines. a Western blot analysis of STAT3 activation in HaP-T1 cells treated with 20 ng/ml of recombinant hOSM at the indicated times. b mRNA levels of IL-15Rα and ICAM1, determined by qRT-PCR in HaP-T1 and PANC-1 cell lines treated with 20 ng/ml of recombinant hOSM at the indicated times. c Concentration of hOSM in the conditioned medium of HaP-T1 cells infected for 24 h with the indicated MOIs of Ad-OSM and NDV-OSM, determined by ELISA. d Western blot analysis of STAT1 activation in HuH-7 cells treated with conditioned medium from HaP-T1 cells infected or not with Ad-OSM, NDV-OSM or their respective GFP-expressing controls. e mRNA levels of IL-15Rα and ICAM1, determined by qRT-PCR, in HaP-T1 and PANC-1 cell lines treated for 24 h with the same conditioned media (representative results of at least 2 experiments performed in triplicate). ** p
Article Snippet: Cells Syrian hamster cells HaP-T1 (DSMZ ACC 222) and H2T (courtesy of Dr. CM Townsend, University of Texas Medical Branch, TX, USA), and human cell lines HuH-7 (JCRB Genebank, Japan), Hep2 (ATCC CCL-23), A549 (ATCC CCL-185), BxPC-3 (ATCC CRL-1687) and PANC-1 (ATCC CRL-1469), were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10 % fetal calf serum, 100 μg/ml streptomycin and 100 units/ml penicillin.
Techniques: Western Blot, Activation Assay, Recombinant, Quantitative RT-PCR, Concentration Assay, Infection, Enzyme-linked Immunosorbent Assay, Expressing