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α cx45 cl acc 207 (Alomone Labs)

Alomone Labs α cx45 cl acc 207

Sequence alignment of the A333-N361 region using Clustal Omega to all available <t>Cx45</t> RefSeq sequences revealed a high degree of conservation within this region, with 72.4% identical, 20.6% conserved, and 6.9% non-conserved residues. For clarity, the sites of point mutations in the Cx45 6E construct are indicated by the single letter code above the alignments. Degree of site conservation (excluding Cx45 6E mutations) is indicated at the bottom, * = identical, : and . = high conservation and intermediate conservation, respectively.

α Cx45 Cl Acc 207, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/α cx45 cl acc 207/product/Alomone Labs
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99

α cx45 cl acc 207 - by Bioz Stars, 2023-09

90/100 stars

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nb4 acc207 (DSMZ)

DSMZ nb4 acc207

Nb4 Acc207, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nb4 acc207/product/DSMZ
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

nb4 acc207 - by Bioz Stars, 2023-09

93/100 stars

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nb4 (DSMZ)

DSMZ nb4

Nb4, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nb4/product/DSMZ
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

nb4 - by Bioz Stars, 2023-09

86/100 stars

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nb4 acc 207 cells (DSMZ)

DSMZ nb4 acc 207 cells

Nb4 Acc 207 Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nb4 acc 207 cells/product/DSMZ
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

nb4 acc 207 cells - by Bioz Stars, 2023-09

86/100 stars

Buy from Supplier

acc 207 human (ATCC)

ATCC acc 207 human

Acc 207 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acc 207 human/product/ATCC
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

acc 207 human - by Bioz Stars, 2023-09

86/100 stars

Buy from Supplier

Image Search Results

Sequence alignment of the A333-N361 region using Clustal Omega to all available Cx45 RefSeq sequences revealed a high degree of conservation within this region, with 72.4% identical, 20.6% conserved, and 6.9% non-conserved residues. For clarity, the sites of point mutations in the Cx45 6E construct are indicated by the single letter code above the alignments. Degree of site conservation (excluding Cx45 6E mutations) is indicated at the bottom, * = identical, : and . = high conservation and intermediate conservation, respectively.

Journal: Journal of molecular and cellular cardiology

Article Title: Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization

doi: 10.1016/j.yjmcc.2017.07.010

Figure Lengend Snippet: Sequence alignment of the A333-N361 region using Clustal Omega to all available Cx45 RefSeq sequences revealed a high degree of conservation within this region, with 72.4% identical, 20.6% conserved, and 6.9% non-conserved residues. For clarity, the sites of point mutations in the Cx45 6E construct are indicated by the single letter code above the alignments. Degree of site conservation (excluding Cx45 6E mutations) is indicated at the bottom, * = identical, : and . = high conservation and intermediate conservation, respectively.

Article Snippet: Antibodies and detection reagents α-Cx45-CT(MAB3101), α-mouse-HRP (H+L; 12-439), α-pS (05-1000), and α-Nedd4 (07-049) were purchased from EMD Millipore; α-Cx43 (C6219), α-β-catenin (C2206), and α-β-tubulin (T5201) were purchased from Sigma-Aldrich; α-E-cadherin-Alexa488 (#3195), α-HA-647 (#3444), α-Myc-555 (#3756), α-rabbit-Alexa555 (#4413), α-mouse-Alexa647 (#4410), Normal Rabbit IgG (#2729), and α-pY-1000 Multi-Mab (#8954) were purchased from Cell Signaling; α-mouse-Alexa488 (A-11001), Texas Red Dextran (10,000 Da; D1863), Lucifer yellow CH (L453), and Streptavidin-Alexa647 ( {"type":"entrez-protein","attrs":{"text":"S21374","term_id":"99986","term_text":"pir||S21374"}} S21374 ) were purchased from Life Technologies; α-rabbit-HRP (H+L; #20320) was purchased from Alpha Diagnostics; Neurobiotin (SP-1120) was purchased from Vector Labs; α-rabbit-HRP (Light chain; #211-032-171) was purchased from Jackson Immuno Research; α-Cx45-CL (ACC-207) was purchased from Alomone; Veriblot-HRP (ab131366) was purchased from Abcam; and DAPI (#02157574) was purchased from MP Biomedicals.

Techniques: Sequencing, Construct

Three different MDCK cells expressing either Cx45 WT or 6E were assessed for expression at the protein and transcript levels. A) Western blots and quantification of Cx45 WT, Cx45 6E, and Cx43 expression. B) RT-PCR gel images and quantification of Cx45 WT and 6E transcripts. C) Western blots and quantification of Cx45 WT or 6E subjected to a 12 hr cycloheximide chase. Results presented as the mean + s.e.m. (n=3). Statistics are used to analyze the data were two-tailed unpaired Student’s T-test (A, n=3, *P<0.05; and C, n=3, n.s., *P<0.9056) and one-way ANOVA with a Neuman-Keuls post hoc test (B, n=3; *P<0.05, **P<0.001 comparing Cx45 WT and 6E, and ##P<0.001 compared to time = 0).

Journal: Journal of molecular and cellular cardiology

Article Title: Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization

doi: 10.1016/j.yjmcc.2017.07.010

Figure Lengend Snippet: Three different MDCK cells expressing either Cx45 WT or 6E were assessed for expression at the protein and transcript levels. A) Western blots and quantification of Cx45 WT, Cx45 6E, and Cx43 expression. B) RT-PCR gel images and quantification of Cx45 WT and 6E transcripts. C) Western blots and quantification of Cx45 WT or 6E subjected to a 12 hr cycloheximide chase. Results presented as the mean + s.e.m. (n=3). Statistics are used to analyze the data were two-tailed unpaired Student’s T-test (A, n=3, *P<0.05; and C, n=3, n.s., *P<0.9056) and one-way ANOVA with a Neuman-Keuls post hoc test (B, n=3; *P<0.05, **P<0.001 comparing Cx45 WT and 6E, and ##P<0.001 compared to time = 0).

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test

Representative immunofluorescence images (red) of methanol fixed cells stably expressing Cx45 WT (left) or 6E (right). Co-immunostaining (green) for either E-Cadherin (MDCK) or β-catenin (Hek-293T) was used to mark the plasma membranes of the fixed cells (blue = DAPI). A) Clonally selected MDCK cells stably expressing mouse Cx45 WT or 6E. B) Bulk selected MDCK cells stably expressing human Cx45 WT or 6E. C) Bulk selected Hek-293T cells stably expressing mouse Cx45 WT and 6E. D) Isolated time-lapse frames of clonally selected MDCK cells expressing C-terminal eGFP fusions of mouse Cx45 WT or 6E (green). White arrows in the insets indicate gap junction plaques. Scale bar A–C = 20 μm and D = 10 μm.

Journal: Journal of molecular and cellular cardiology

Article Title: Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization

doi: 10.1016/j.yjmcc.2017.07.010

Figure Lengend Snippet: Representative immunofluorescence images (red) of methanol fixed cells stably expressing Cx45 WT (left) or 6E (right). Co-immunostaining (green) for either E-Cadherin (MDCK) or β-catenin (Hek-293T) was used to mark the plasma membranes of the fixed cells (blue = DAPI). A) Clonally selected MDCK cells stably expressing mouse Cx45 WT or 6E. B) Bulk selected MDCK cells stably expressing human Cx45 WT or 6E. C) Bulk selected Hek-293T cells stably expressing mouse Cx45 WT and 6E. D) Isolated time-lapse frames of clonally selected MDCK cells expressing C-terminal eGFP fusions of mouse Cx45 WT or 6E (green). White arrows in the insets indicate gap junction plaques. Scale bar A–C = 20 μm and D = 10 μm.

Techniques: Immunofluorescence, Stable Transfection, Expressing, Immunostaining, Isolation

$Triton X-100 solubility was used to compare MDCK cells expressing Cx45 WT or 6E. A) Western blot image of Triton X-100 extracted protein; total lysate (T), soluble (S), and insoluble (I) fractions. Below is the quantification of protein in the T, S, and I fractions. Statistics represent one-way ANOVA with a Neuman-Keuls post-hoc correction (n=3, **P<0.001,***P<0.0001). B) Representative fluorescent images (left) and quantification (right) of in situ Triton X-100 and mock (1x PBS) extracted Cx45 WT or 6E in MDCK cells (red, Cx45; blue, DAPI). C) MDCK cells expressing Cx45 WT (top) or 6E (bottom) were scrape loaded with tracer dyes to assess the degree of intercellular communication. Red, Texas red dextran (MW 10,000 Da); yellow, Lucifer yellow CH (MW 443 Da; −2 charge), green, neurobiotin (MW 287 Da; +1 charge); and blue, DAPI. Images are representative of greater than six independent experiments. Scale bar in B = 20 μm and C = 100 μm.$

Journal: Journal of molecular and cellular cardiology

Article Title: Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization

doi: 10.1016/j.yjmcc.2017.07.010

Figure Lengend Snippet: Triton X-100 solubility was used to compare MDCK cells expressing Cx45 WT or 6E. A) Western blot image of Triton X-100 extracted protein; total lysate (T), soluble (S), and insoluble (I) fractions. Below is the quantification of protein in the T, S, and I fractions. Statistics represent one-way ANOVA with a Neuman-Keuls post-hoc correction (n=3, **P<0.001,***P<0.0001). B) Representative fluorescent images (left) and quantification (right) of in situ Triton X-100 and mock (1x PBS) extracted Cx45 WT or 6E in MDCK cells (red, Cx45; blue, DAPI). C) MDCK cells expressing Cx45 WT (top) or 6E (bottom) were scrape loaded with tracer dyes to assess the degree of intercellular communication. Red, Texas red dextran (MW 10,000 Da); yellow, Lucifer yellow CH (MW 443 Da; −2 charge), green, neurobiotin (MW 287 Da; +1 charge); and blue, DAPI. Images are representative of greater than six independent experiments. Scale bar in B = 20 μm and C = 100 μm.

Techniques: Solubility, Expressing, Western Blot, In Situ

MDCK cells stably expressing Cx45 WT or 6E were subjected to conditions that activate hemichannels activity (Ca2+ deprivation) and the level of neurobiotin uptake was measured. Representative fluorescent micrographs of cells loaded with neurobiotin and probed with streptavidin-647 conjugate (red). Quantification of DAPI (blue) normalized mean fluorescent intensity (MFI) from 3 replicates, 15 random 100X FOVs were acquired per replicate. Statistics are one-way ANOVA with a Neuman-Keuls multiple comparison test (***P<0.0001 relative to +Ca2+ within same group, ^ and ^^^P <0.05 and <0.0001 respectively, relative to −Ca2+ within same group; ###P<0.0001 between −Ca2+ treatment groups; •••P<0.0001 compared to WT + pervanadate). Scale bar = 50 μm.

Journal: Journal of molecular and cellular cardiology

Article Title: Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization

doi: 10.1016/j.yjmcc.2017.07.010

Figure Lengend Snippet: MDCK cells stably expressing Cx45 WT or 6E were subjected to conditions that activate hemichannels activity (Ca2+ deprivation) and the level of neurobiotin uptake was measured. Representative fluorescent micrographs of cells loaded with neurobiotin and probed with streptavidin-647 conjugate (red). Quantification of DAPI (blue) normalized mean fluorescent intensity (MFI) from 3 replicates, 15 random 100X FOVs were acquired per replicate. Statistics are one-way ANOVA with a Neuman-Keuls multiple comparison test (***P<0.0001 relative to +Ca2+ within same group, ^ and ^^^P <0.05 and <0.0001 respectively, relative to −Ca2+ within same group; ###P<0.0001 between −Ca2+ treatment groups; •••P<0.0001 compared to WT + pervanadate). Scale bar = 50 μm.

Techniques: Stable Transfection, Expressing, Activity Assay

MDCK cells stably expressing Cx45 WT or 6E were cultured, lysed, and immunoprecipitated to assess total phosphorylation levels and interactions with β-tubulin, Drebrin, and Nedd4. A) Representative Western blots of immunoprecipitated Cx45 WT or 6E blotting with a general anti-pY antibody. (n=3, ** P<0.01) Representative Western blot of B) β-tubulin (n=7, ***P<0.001), C) Drebrin (n=3, **P<0.01), and D) Nedd4 (n=3, **P<0.01) co-immunoprecipitated with either Cx45 WT or 6E. Data presented as mean fold change + s.e.m. relative to the first WT replicate. Statistics are two-tailed unpaired Student’s T-test.

Journal: Journal of molecular and cellular cardiology

Article Title: Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization

doi: 10.1016/j.yjmcc.2017.07.010

Figure Lengend Snippet: MDCK cells stably expressing Cx45 WT or 6E were cultured, lysed, and immunoprecipitated to assess total phosphorylation levels and interactions with β-tubulin, Drebrin, and Nedd4. A) Representative Western blots of immunoprecipitated Cx45 WT or 6E blotting with a general anti-pY antibody. (n=3, ** P<0.01) Representative Western blot of B) β-tubulin (n=7, ***P<0.001), C) Drebrin (n=3, **P<0.01), and D) Nedd4 (n=3, **P<0.01) co-immunoprecipitated with either Cx45 WT or 6E. Data presented as mean fold change + s.e.m. relative to the first WT replicate. Statistics are two-tailed unpaired Student’s T-test.

Techniques: Stable Transfection, Expressing, Cell Culture, Immunoprecipitation, Western Blot, Two Tailed Test

Oocytes injected with equal amounts of cRNA coding for Cx45 WT or the 6E mutant were voltage clamped and hemichannel currents elicited by large depolarizing voltage steps from −50 to +80 mV in 10 mV incrememts from a holding potential of −80 mV. A) Representation of voltage protocol. Under the same voltage protocols, the WT channels mediated substantially less current than the 6E channels. Representative current plots from (B) WT and (C) 6E expressing oocytes.

Journal: Journal of molecular and cellular cardiology

Article Title: Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization

doi: 10.1016/j.yjmcc.2017.07.010

Figure Lengend Snippet: Oocytes injected with equal amounts of cRNA coding for Cx45 WT or the 6E mutant were voltage clamped and hemichannel currents elicited by large depolarizing voltage steps from −50 to +80 mV in 10 mV incrememts from a holding potential of −80 mV. A) Representation of voltage protocol. Under the same voltage protocols, the WT channels mediated substantially less current than the 6E channels. Representative current plots from (B) WT and (C) 6E expressing oocytes.

Techniques: Injection, Mutagenesis, Expressing

ACC-207 Search Results

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