Journal: Scientific Reports
Article Title: SHOC2 scaffold protein modulates daunorubicin-induced cell death through p53 modulation in lymphoid leukemia cells
Figure Lengend Snippet: ( a , b ) Cell viability of Nalm-6 and Reh cells stable transduced with shRNA for SHOC2 or SCR control were evaluated in a 4-day MTT assay after treatment with increasing concentrations of Daunorubicin (10ηM, 25ηM, 50ηM, 100ηM and 150ηM). Results are expressed as mean absorbance [A475 nm–A650 nm] with standard deviation (S.D.) from three independent experiments. Representative viability curves after 72 h of Daunorubicin exposure show how SHOC2 absence has greatly increased daunorubicin resistance (* p < 0.05). ( c , d ) SHOC2 inhibition cleared impaired apoptosis-induced cell death in both Reh and Nalm-6 cells (*** p < 0.001) after Daunorubicin treatment for 72 h (respective IC 50 values). The bar-graphs represent mean with S.D. from three independent experiments. Statistically significant analyses are indicated by asterisks: * p < 0.05, ** p < 0.01.
Article Snippet: The commercial human Leukemic cell lines Nalm-6 (DSMZ ACC128), Reh (ATCC CRL-8286) and Jurkat (ATCC TIB-152) were cultured in RPMI 1640 medium (Corning, Manassas, VA, USA) supplemented with 10% fetal bovine serum (Gibco BRL, Carlsbad, CA, USA), penicillin (100 U/mL) and streptomycin (100 μg/mL) in a humidified atmosphere with 5%CO 2 at 37 °C, as recommended by ATCC.
Techniques: Transduction, shRNA, MTT Assay, Standard Deviation, Inhibition