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    Alomone Labs acc 029
    Paclitaxel increased immunoreactivity for <t>TRPV1</t> and TRPM8 in sensory neurons. Immunocytochemical staining of cultured DRG neurons treated with vehicle or paclitaxel using <t>anti‐TRPV1,</t> anti‐TRPA1 or anti‐TRPM8 antibodies along with the neuronal marker NeuN and DAPI staining. Green: TRPV1 (a, b), TRPA1 (d, e) or TRPM8 (g and h). Red: NeuN. DAPI: cyan. Scale bar denotes 20 μm. (c, f and i) Mean fluorescence intensity of TRPV1 (c), TRPA1 (f) or TRPM8 (i) immunoreactivity for vehicle and paclitaxel treated neurons. Data were collected 48 h post‐treatment. Data are presented as individual values with means ± SD; N = 7. * P
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    Paclitaxel increased immunoreactivity for TRPV1 and TRPM8 in sensory neurons. Immunocytochemical staining of cultured DRG neurons treated with vehicle or paclitaxel using anti‐TRPV1, anti‐TRPA1 or anti‐TRPM8 antibodies along with the neuronal marker NeuN and DAPI staining. Green: TRPV1 (a, b), TRPA1 (d, e) or TRPM8 (g and h). Red: NeuN. DAPI: cyan. Scale bar denotes 20 μm. (c, f and i) Mean fluorescence intensity of TRPV1 (c), TRPA1 (f) or TRPM8 (i) immunoreactivity for vehicle and paclitaxel treated neurons. Data were collected 48 h post‐treatment. Data are presented as individual values with means ± SD; N = 7. * P

    Journal: British Journal of Pharmacology

    Article Title: Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats). Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats

    doi: 10.1111/bph.15809

    Figure Lengend Snippet: Paclitaxel increased immunoreactivity for TRPV1 and TRPM8 in sensory neurons. Immunocytochemical staining of cultured DRG neurons treated with vehicle or paclitaxel using anti‐TRPV1, anti‐TRPA1 or anti‐TRPM8 antibodies along with the neuronal marker NeuN and DAPI staining. Green: TRPV1 (a, b), TRPA1 (d, e) or TRPM8 (g and h). Red: NeuN. DAPI: cyan. Scale bar denotes 20 μm. (c, f and i) Mean fluorescence intensity of TRPV1 (c), TRPA1 (f) or TRPM8 (i) immunoreactivity for vehicle and paclitaxel treated neurons. Data were collected 48 h post‐treatment. Data are presented as individual values with means ± SD; N = 7. * P

    Article Snippet: Next, membranes were incubated at 4°C overnight with the following primary antibodies diluted in 1% BSA in TBST: rabbit anti‐Actin, 1:1000 (Sigma‐Aldrich, Cat# A2066, RRID:AB_476693); rabbit anti‐TRPV1, 1:1000 (Alomone Labs, Cat# ACC‐029); rabbit anti‐TRPM8, 1:500 (Alomone Labs, Cat# ACC‐049); rabbit anti‐NaV 1.7, 1:2500 (Alomone Labs, Cat# ASC‐008); and rabbit anti‐NaV1.8, 1:1000 (Alomone Labs, Cat# ASC‐016), respectively.

    Techniques: Staining, Cell Culture, Marker, Fluorescence

    Paclitaxel increases TRPV1 and TRPM8 channel activity in IB4(−) neurons. (a, c and e) Representative inward current responses to a 1 μM capsaicin (a), 100 μM AITC (c) and 100 μM menthol (e) of IB4(−) and IB4(+) neurons exposed to vehicle or paclitaxel. Calibration bars are for all recordings. TRPM8 channel activity was further confirmed by using the antagonist 10 μM AMTB along with the menthol pulse. (b, d, and f) Peak current density observed after application of capsaicin (b), AITC (d) or menthol (f). AITC current was evoked in the same cells as capsaicin, after application of 4 capsaicin pulses. To quantify TRPM8 responses the remaining inward current during co‐application of menthol and AMTB was subtracted from the peak response to menthol. Data are expressed as medians (with interquartile range) or as mean ± SD. * P

    Journal: British Journal of Pharmacology

    Article Title: Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats). Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats

    doi: 10.1111/bph.15809

    Figure Lengend Snippet: Paclitaxel increases TRPV1 and TRPM8 channel activity in IB4(−) neurons. (a, c and e) Representative inward current responses to a 1 μM capsaicin (a), 100 μM AITC (c) and 100 μM menthol (e) of IB4(−) and IB4(+) neurons exposed to vehicle or paclitaxel. Calibration bars are for all recordings. TRPM8 channel activity was further confirmed by using the antagonist 10 μM AMTB along with the menthol pulse. (b, d, and f) Peak current density observed after application of capsaicin (b), AITC (d) or menthol (f). AITC current was evoked in the same cells as capsaicin, after application of 4 capsaicin pulses. To quantify TRPM8 responses the remaining inward current during co‐application of menthol and AMTB was subtracted from the peak response to menthol. Data are expressed as medians (with interquartile range) or as mean ± SD. * P

    Article Snippet: Next, membranes were incubated at 4°C overnight with the following primary antibodies diluted in 1% BSA in TBST: rabbit anti‐Actin, 1:1000 (Sigma‐Aldrich, Cat# A2066, RRID:AB_476693); rabbit anti‐TRPV1, 1:1000 (Alomone Labs, Cat# ACC‐029); rabbit anti‐TRPM8, 1:500 (Alomone Labs, Cat# ACC‐049); rabbit anti‐NaV 1.7, 1:2500 (Alomone Labs, Cat# ASC‐008); and rabbit anti‐NaV1.8, 1:1000 (Alomone Labs, Cat# ASC‐016), respectively.

    Techniques: Activity Assay