Article Title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals
Figure Lengend Snippet: Quantification of the sub-synaptic localization of presynaptic Cav2.3, GBRs and KCTDs along both MHb-IPN pathways Transmission electron microscopy images of 70 nm−thick sections following pre-embedding immunolabeled IPN slices for Cav2.3 ( A ), GABA B1 ( B ), KCTD8 ( C ), KCTD12 ( D ) and KCTD12b ( E ) from synapses in the rostral (left images) and lateral (right image) IPN subnuclei. Scale bars: 200 nm. Graph on the right displays quantification of relative and absolute silver-enhanced gold particle densities in the active zone and at distances of 50 − 200 nm from the edge of the active zone (50 nm bins). F Absolute labeling densities are summarized for synapses in the rostral (left panel) and lateral IPN (right panel). Note absence of KCTD12 and KCTD12b particles in presynaptic terminals inside the lateral IPN subnuclei. KCTD12 was not included in panel F because of predominantly postsynaptic localization inside the rostral IPN. Data was pooled from two animals, showing no significant difference in gold particle distribution patterns with Kolmogorov-Smirnov test (see Supplementary Figure S1).
Article Snippet: Thereafter, lysates were incubated by rotating for 16 h at 4 °C in the presence of 2.5 μl of 0.3 μg/μl anti-Cav2.3 (CACNA1E) antibody (ACC-006, Alomone Labs, Jerusalem, Israel).
Techniques: Transmission Assay, Electron Microscopy, Immunolabeling, Labeling