Article Title: CACNB4 overexpression decreases dendritic spine density in sex-specific manner
Figure Lengend Snippet: β subunit protein levels. A) (Left) Mean optical density (a.u.) of β4 was significantly lower in P84, relative to P28 male and female mice (F=9.635, DF=3, p=0.013), with no significant sex or age by sex interactions. Error bars = SEM. (Right) Representative blot showing β4 bands at ~51-55kD. B) (Left) Mean optical density (a.u.) of β1 was significantly lower in P84, relative to P28 male and female mice (F=21.499, DF=3, p=0.001). There was also a main effect of sex (F=6.944, DF=3, p=0.027) and a significant age by sex interaction (F=6.835, DF=3, p=0 .028). Main effect of sex was significant at P28 (F=12.138, DF=1, p=0.040) but not P84 (F=0.016, DF=1, p=0.906). Error bars = SEM. (Right) Representative blot showing β1 bands at ~50-80kD. C) (Left) Neither age nor sex significantly impacted mean optical density (a.u.) of β3 and there was not a significant sex by age interaction. Error bars = SEM. (Right) Representative blot showing β3 bands at ~55kD (molecular weight of the predominant β3 isoform).
Article Snippet: The membrane was incubated overnight in Pierce SuperBlock Blocking Buffer and validated primary antibodies: either mouse anti-CaV β1 (Neuromab #73-052 1:100), rabbit anti-CaV β3 (Alomone Labs #AAC-008, 1:800) or mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000).
Techniques: Mouse Assay, Molecular Weight