Journal: Experimental and Therapeutic Medicine

Article Title: PP2Ac knockdown attenuates lipotoxicity‑induced pancreatic β‑cell dysfunction and apoptosis

doi: 10.3892/etm.2023.12247

Figure Lengend Snippet: PP2Ac knockdown attenuates PA-induced apoptosis in MIN6 cells. (A) PP2Ac knockdown increases the resistance of MIN6 cells to PA; cell viability decreased following the addition of various concentrations of PA, but the decline in viability was less in the sh2 group than in the shc group. (B) Exposure of the cells to 0.5 mM PA for 12, 24, 36 and 48 h, reduced cell viability, but the cell viability of the sh2 group was always higher than that of the shc group. (C) Apoptosis of the MIN6 cells was detected by flow cytometry. (D) Expression levels of the apoptosis-associated proteins caspase 3, Bcl-2 and Bax in the transfected MIN6 cells were analyzed by western blotting, using β-actin as a loading control. All experiments were performed at least three times, and values are reported as the mean ± standard deviation. * P<0.05, ** P<0.01. PP2Ac, catalytic subunit of protein phosphatase 2A; PA, palmitate; MIN6, mouse insulinoma 6; shc, short hairpin RNA control; sh2, PP2AC short hairpin RNA 2; ns, not significant.

Article Snippet: An Annexin V-APC/PI Apoptosis Detection Kit (cat. no. A30813; MultiSciences Biotech Co., Ltd.) was used to detect the cell apoptosis rate.

Techniques: Knockdown, Flow Cytometry, Expressing, Transfection, Western Blot, Control, Standard Deviation, shRNA