Journal: Cells

Article Title: Type II Collagen-Specific B Cells Induce Immune Tolerance in Th1-Skewed, Th2-Skewed, and Arthritis-Prone Strains of Mice

doi: 10.3390/cells10040870

Figure Lengend Snippet: In vitro generated CII-specific ACAID B cells inhibited CII-induced DTH responses in DBA/1 mice. The in vitro generation of CII-specific ACAID B cells and the DTH assay were described in the Materials and Methods section. CII-specific ACAID B cells were intravenously injected to induce ACAID followed by subcutaneous immunization with CII/CFA on day 7. On day 14, mice were challenged with CII (500 μg in 20 μL) intradermally in the left ear pinna, and 20 μL of acetic acid alone was injected into the right ear pinna as an internal control. ACAID induction was confirmed by suppression of ear-swelling responses 24 h (left panel) and 48 h (right panel). The positive control mice were subcutaneously immunized with CII/CFA on day 7 and with CII on day 14, whereas the negative control mice only received intradermal injection of CII on day 14. * p

Article Snippet: Generation of ACAID APCs Generation of ACAID APCs in vitro from C57BL/6, BALB/c, or DBA/1 mice was done as described earlier [ ].

Techniques: In Vitro, Generated, Mouse Assay, Injection, Positive Control, Negative Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Cutting Edge: Roles of Toll-Like Receptor 4 and IL-23 in IL-17 Expression in Response to Klebsiella pneumoniae Infection

doi:

Figure Lengend Snippet: CD4 + and CD8 + T cell IL-17 response to conditioned medium from K. pneumoniae -pulsed DC requires biologically active IL-23. A , Conditioned medium from p35 −/− (knockout (K/O)) DC (devoid of IL-12) elicits an augmented IL-17 response from CD4 + and CD8 + T cells, whereas medium from p40 −/− DC (devoid of IL-12 and IL-23) shows marked attenuation in comparison to wild type (WT). Neutralization of conditioned medium with anti-p40 also showed significantly less IL-17 induction in both CD4 + and CD8 + T cells. B , IL-17 mRNA copy number from T cells of the same experiment ( n = 6 per group; columns with different uppercase (CD4 + ) or lowercase (CD8 + ) letters denote statistical significance ( p

Article Snippet: For experiments designed to test the role of IL-23, Ab directed against mouse p40, a subunit common to IL-12 and IL-23, or isotype control, was incubated for 1 h with conditioned medium before T cell addition (R & D Systems).

Techniques: Knock-Out, Neutralization