Article Title: SIRT2 Suppresses Adipocyte Differentiation by Deacetylating FOXO1 and Enhancing FOXO1's Repressive Interaction with PPAR?
Figure Lengend Snippet: SIRT2 interacts with FOXO1 and deacetylates FOXO1. (A) HEK293 cells were transiently transfected with FOXO1, SIRT2-Flag, or both by the calcium phosphate method. Forty hours after transfection, cells were lysed, and SIRT2-Flag proteins were immunoprecipitated
Article Snippet: The following antibodies were used: anti-SIRT2 (Santa Cruz Biotechnology, Santa Cruz, CA; sc-20966, 1:1000), anti-actin (Santa Cruz, sc-1616, 1:1000), anti-FOXO1 (Santa Cruz, sc-11350, 1:1000), anti-PPARγ (Santa Cruz, sc-7196, sc-7273, 1:500), anti-GAPDH (Ambion, Austin, TX; 4300, 1:500,000), anti-Lamin A/C (Cell Signaling, Beverly, MA; 4056, 1:1000), anti-acetylated lysine (Cell Signaling, 9441, 1:1000), anti-α-tubulin (Sigma, St. Louis, MO; T5168, 1:100,000), HRP-conjugated anti-mouse (Bio-Rad, Richmond, CA; 170-6516, 1:3000), anti-rabbit (Bio-Rad, 170-6515, 1:3000), and anti-rabbit IgG-native (Sigma, R3155, 1:2000) antibodies.
Techniques: Transfection, Immunoprecipitation