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    Santa Cruz Biotechnology anti foxo1
    SIRT2 increases <t>FOXO1</t> repressive binding to PPARγ. (A) HEK293T cells were transfected with Flag-FOXO1 and pSV-PPARγ, pCMV-SIRT2, or pCMV-SIRT2N168A. Cells were treated with 1 μM tricostatin A for 4 h before harvest. FOXO1 was immunoprecipitated
    Anti Foxo1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti foxo1 - by Bioz Stars, 2021-10
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    SIRT2 increases FOXO1 repressive binding to PPARγ. (A) HEK293T cells were transfected with Flag-FOXO1 and pSV-PPARγ, pCMV-SIRT2, or pCMV-SIRT2N168A. Cells were treated with 1 μM tricostatin A for 4 h before harvest. FOXO1 was immunoprecipitated

    Journal:

    Article Title: SIRT2 Suppresses Adipocyte Differentiation by Deacetylating FOXO1 and Enhancing FOXO1's Repressive Interaction with PPAR?

    doi: 10.1091/mbc.E08-06-0647

    Figure Lengend Snippet: SIRT2 increases FOXO1 repressive binding to PPARγ. (A) HEK293T cells were transfected with Flag-FOXO1 and pSV-PPARγ, pCMV-SIRT2, or pCMV-SIRT2N168A. Cells were treated with 1 μM tricostatin A for 4 h before harvest. FOXO1 was immunoprecipitated

    Article Snippet: The following antibodies were used: anti-SIRT2 (Santa Cruz Biotechnology, Santa Cruz, CA; sc-20966, 1:1000), anti-actin (Santa Cruz, sc-1616, 1:1000), anti-FOXO1 (Santa Cruz, sc-11350, 1:1000), anti-PPARγ (Santa Cruz, sc-7196, sc-7273, 1:500), anti-GAPDH (Ambion, Austin, TX; 4300, 1:500,000), anti-Lamin A/C (Cell Signaling, Beverly, MA; 4056, 1:1000), anti-acetylated lysine (Cell Signaling, 9441, 1:1000), anti-α-tubulin (Sigma, St. Louis, MO; T5168, 1:100,000), HRP-conjugated anti-mouse (Bio-Rad, Richmond, CA; 170-6516, 1:3000), anti-rabbit (Bio-Rad, 170-6515, 1:3000), and anti-rabbit IgG-native (Sigma, R3155, 1:2000) antibodies.

    Techniques: Binding Assay, Transfection, Immunoprecipitation

    SIRT2 interacts with FOXO1 and deacetylates FOXO1. (A) HEK293 cells were transiently transfected with FOXO1, SIRT2-Flag, or both by the calcium phosphate method. Forty hours after transfection, cells were lysed, and SIRT2-Flag proteins were immunoprecipitated

    Journal:

    Article Title: SIRT2 Suppresses Adipocyte Differentiation by Deacetylating FOXO1 and Enhancing FOXO1's Repressive Interaction with PPAR?

    doi: 10.1091/mbc.E08-06-0647

    Figure Lengend Snippet: SIRT2 interacts with FOXO1 and deacetylates FOXO1. (A) HEK293 cells were transiently transfected with FOXO1, SIRT2-Flag, or both by the calcium phosphate method. Forty hours after transfection, cells were lysed, and SIRT2-Flag proteins were immunoprecipitated

    Article Snippet: The following antibodies were used: anti-SIRT2 (Santa Cruz Biotechnology, Santa Cruz, CA; sc-20966, 1:1000), anti-actin (Santa Cruz, sc-1616, 1:1000), anti-FOXO1 (Santa Cruz, sc-11350, 1:1000), anti-PPARγ (Santa Cruz, sc-7196, sc-7273, 1:500), anti-GAPDH (Ambion, Austin, TX; 4300, 1:500,000), anti-Lamin A/C (Cell Signaling, Beverly, MA; 4056, 1:1000), anti-acetylated lysine (Cell Signaling, 9441, 1:1000), anti-α-tubulin (Sigma, St. Louis, MO; T5168, 1:100,000), HRP-conjugated anti-mouse (Bio-Rad, Richmond, CA; 170-6516, 1:3000), anti-rabbit (Bio-Rad, 170-6515, 1:3000), and anti-rabbit IgG-native (Sigma, R3155, 1:2000) antibodies.

    Techniques: Transfection, Immunoprecipitation