Journal: Antioxidants

Article Title: An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp ( Ctenopharyngodon idella )

doi: 10.3390/antiox11050806

Figure Lengend Snippet: The effect of MOS on ZO-1 expression by the immunohistochemistry method in three intestinal segments after infection with Aeromonas hydrophila. ( A ) ZO-1 protein expression of intestine in the 0 mg/kg MOS, 400 mg/kg MOS and 1000 mg/kg MOS groups; ( B ) Quantification of the positive area as revealed by Image Pro Plus 6.0. N = 6 for each MOS level. Differences among the variables were assessed using Student’s t -tests. Statistical significance: * p < 0.05; ** p < 0.01, *** p < 0.001; **** p < 0.0001; ns: not significant.

Article Snippet: The information on antibodies used for IHC is as follows: ZO-1 (A0659, 1:100, ABclonal) and Occludin (A2601, 1:100, ABclonal).

Techniques: Expressing, Immunohistochemistry, Infection