A03742 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Boster Bio α sma
    Primer sequences for RT-PCR (mouse)
    α Sma, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α sma/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α sma - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    95
    ATCC brucella species
    Primer sequences for RT-PCR (mouse)
    Brucella Species, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brucella species/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brucella species - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    99
    PerkinElmer totalseq a0374 anti human cd98 mem 108

    Totalseq A0374 Anti Human Cd98 Mem 108, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/totalseq a0374 anti human cd98 mem 108/product/PerkinElmer
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    totalseq a0374 anti human cd98 mem 108 - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    94
    Proteintech anti sglt2
    High-salt diet alters <t>SGLT2</t> and Na+/K + -ATPase expression in renal tubules of DKD mice. (A) Expression of SGLT2 gene in renal tubules ( n = 6 per group). (B) Paraffin-embedded renal sections were stained with SGLT2, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (C) Histopathological assessment of SGLT2, ATP1A1 and ATP1B1 proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; ## p < 0.01 and ### p < 0.001 vs. DM group.
    Anti Sglt2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sglt2/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti sglt2 - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    86
    Boster Bio a smooth muscle actin a sma
    High-salt diet alters <t>SGLT2</t> and Na+/K + -ATPase expression in renal tubules of DKD mice. (A) Expression of SGLT2 gene in renal tubules ( n = 6 per group). (B) Paraffin-embedded renal sections were stained with SGLT2, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (C) Histopathological assessment of SGLT2, ATP1A1 and ATP1B1 proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; ## p < 0.01 and ### p < 0.001 vs. DM group.
    A Smooth Muscle Actin A Sma, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a smooth muscle actin a sma/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a smooth muscle actin a sma - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    Image Search Results


    Primer sequences for RT-PCR (mouse)

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction

    doi: 10.1152/ajprenal.00231.2016

    Figure Lengend Snippet: Primer sequences for RT-PCR (mouse)

    Article Snippet: Antibodies to NLRP3 were from Novus, caspase-1 from Abcam, ASC from Santa Cruz Biotechnology, p-AKT (ser437), AKT, and IL-1β from Cell Signaling Technology, fibronectin from Sigma, COX2, α-SMA, collagen I, and collagen III from Boster (Wuhan, China).

    Techniques: Sequencing

    Analysis of mRNA expression by quantitative real-time PCR for α-SMA (A), fibronectin (B), collagen III (C), and TGF-β1 (D) in obstructed kidneys of mPGES-1 WT and KO mice. α-SMA, α-smooth muscle actin; WT, wild type; KO, knockout. WT-Sham, n = 6; WT-7UUO, n = 7; KO-Sham, n = 6; KO-7UUO, n = 7. *P < 0.05 compared with Sham groups. #P < 0.05 compared with WT-7UUO.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction

    doi: 10.1152/ajprenal.00231.2016

    Figure Lengend Snippet: Analysis of mRNA expression by quantitative real-time PCR for α-SMA (A), fibronectin (B), collagen III (C), and TGF-β1 (D) in obstructed kidneys of mPGES-1 WT and KO mice. α-SMA, α-smooth muscle actin; WT, wild type; KO, knockout. WT-Sham, n = 6; WT-7UUO, n = 7; KO-Sham, n = 6; KO-7UUO, n = 7. *P < 0.05 compared with Sham groups. #P < 0.05 compared with WT-7UUO.

    Article Snippet: Antibodies to NLRP3 were from Novus, caspase-1 from Abcam, ASC from Santa Cruz Biotechnology, p-AKT (ser437), AKT, and IL-1β from Cell Signaling Technology, fibronectin from Sigma, COX2, α-SMA, collagen I, and collagen III from Boster (Wuhan, China).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Knock-Out

    Western blot analysis of α-SMA, fibronectin, and collagen III protein expression in obstructed kidneys of mPGES-1 WT and KO mice. A: semiquantitative immunoblots reacted with anti-α-SMA, fibronectin, and collagen III antibodies. GAPDH was used as internal loading control. Corresponding densitometric analyses of protein expression levels of fibronectin (B) and collagen III (C) corrected by GAPDH (B). WT-Sham, n = 6; WT-7UUO, n = 7; KO-Sham, n = 6; KO-7UUO, n = 7. α-SMA, α-smooth muscle actin. *P < 0.05 compared with Sham groups. §P < 0.05 compared with WT-Sham. #P < 0.05 compared with WT-7UUO.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction

    doi: 10.1152/ajprenal.00231.2016

    Figure Lengend Snippet: Western blot analysis of α-SMA, fibronectin, and collagen III protein expression in obstructed kidneys of mPGES-1 WT and KO mice. A: semiquantitative immunoblots reacted with anti-α-SMA, fibronectin, and collagen III antibodies. GAPDH was used as internal loading control. Corresponding densitometric analyses of protein expression levels of fibronectin (B) and collagen III (C) corrected by GAPDH (B). WT-Sham, n = 6; WT-7UUO, n = 7; KO-Sham, n = 6; KO-7UUO, n = 7. α-SMA, α-smooth muscle actin. *P < 0.05 compared with Sham groups. §P < 0.05 compared with WT-Sham. #P < 0.05 compared with WT-7UUO.

    Article Snippet: Antibodies to NLRP3 were from Novus, caspase-1 from Abcam, ASC from Santa Cruz Biotechnology, p-AKT (ser437), AKT, and IL-1β from Cell Signaling Technology, fibronectin from Sigma, COX2, α-SMA, collagen I, and collagen III from Boster (Wuhan, China).

    Techniques: Western Blot, Expressing

    Journal: Cell

    Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

    doi: 10.1016/j.cell.2021.12.018

    Figure Lengend Snippet:

    Article Snippet: TotalSeq-A0374 anti-human CD98 (MEM-108) , BioLegend , 315605; RRID: AB_2750369.

    Techniques: Purification, Recombinant, Staining, Expressing, Software, Microscopy

    High-salt diet alters SGLT2 and Na+/K + -ATPase expression in renal tubules of DKD mice. (A) Expression of SGLT2 gene in renal tubules ( n = 6 per group). (B) Paraffin-embedded renal sections were stained with SGLT2, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (C) Histopathological assessment of SGLT2, ATP1A1 and ATP1B1 proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; ## p < 0.01 and ### p < 0.001 vs. DM group.

    Journal: Frontiers in Pharmacology

    Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

    doi: 10.3389/fphar.2021.741087

    Figure Lengend Snippet: High-salt diet alters SGLT2 and Na+/K + -ATPase expression in renal tubules of DKD mice. (A) Expression of SGLT2 gene in renal tubules ( n = 6 per group). (B) Paraffin-embedded renal sections were stained with SGLT2, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (C) Histopathological assessment of SGLT2, ATP1A1 and ATP1B1 proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; ## p < 0.01 and ### p < 0.001 vs. DM group.

    Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

    Techniques: Expressing, Staining

    High salt alters SGLT2 and Na+/K + -ATPase expression in HG-treated HK-2. (A) Intracellular glucose concentration. (B,C) Expression of SGLT2 in HK-2 in HK-2 cultured with HG and 15 mM NaCl. (D) Expression of NKAIN4, ATP1A1, ATP1B1 and ATP1B3 genes in HK-2 after exposure to HG and different concentrations of NaCl. (E,F) Expression of ATP1A1 protein in HK-2 with HG and 15 mM NaCl cultured. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; # p < 0.05 and ## p < 0.01 vs. HG group. (G) Expression of NKAIN4 and fatty acid metabolism related genes were detected in HK-2 treated with Digoxin. * p < 0.05 and *** p < 0.001 vs. DM + DMSO group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. HG + NaCl + DMSO group. All data are mean ± SEM, n = 3 per group.

    Journal: Frontiers in Pharmacology

    Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

    doi: 10.3389/fphar.2021.741087

    Figure Lengend Snippet: High salt alters SGLT2 and Na+/K + -ATPase expression in HG-treated HK-2. (A) Intracellular glucose concentration. (B,C) Expression of SGLT2 in HK-2 in HK-2 cultured with HG and 15 mM NaCl. (D) Expression of NKAIN4, ATP1A1, ATP1B1 and ATP1B3 genes in HK-2 after exposure to HG and different concentrations of NaCl. (E,F) Expression of ATP1A1 protein in HK-2 with HG and 15 mM NaCl cultured. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; # p < 0.05 and ## p < 0.01 vs. HG group. (G) Expression of NKAIN4 and fatty acid metabolism related genes were detected in HK-2 treated with Digoxin. * p < 0.05 and *** p < 0.001 vs. DM + DMSO group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. HG + NaCl + DMSO group. All data are mean ± SEM, n = 3 per group.

    Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

    Techniques: Expressing, Concentration Assay, Cell Culture

    | High-salt diet attenuates gene and protein expression in diabetic mice treated with dapagliflozin. (A) The detection of ACR ( n = 6 per group). (B) Expression of α Sma, Col-1 and Slc5a2 genes in renal tubules after dapagliflozin treatment ( n = 6 per group). (C) Paraffin-embedded renal sections were stained with FN and SGLT2 antibodies (magnification, 400×, bar = 20 μm). (D) Histopathological assessment of FN and SGLT2 proteins ( n = 4 per group). Expression of genes involved in fatty acid metabolism (E) and mitochondrial biosynthesis (H) in renal tubules after dapagliflozin treatment ( n = 6 per group). (F,I) Paraffin-embedded renal sections were stained with CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (G,J) Histopathological assessment of CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. DM + Saline group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. DM + Dapa group.

    Journal: Frontiers in Pharmacology

    Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

    doi: 10.3389/fphar.2021.741087

    Figure Lengend Snippet: | High-salt diet attenuates gene and protein expression in diabetic mice treated with dapagliflozin. (A) The detection of ACR ( n = 6 per group). (B) Expression of α Sma, Col-1 and Slc5a2 genes in renal tubules after dapagliflozin treatment ( n = 6 per group). (C) Paraffin-embedded renal sections were stained with FN and SGLT2 antibodies (magnification, 400×, bar = 20 μm). (D) Histopathological assessment of FN and SGLT2 proteins ( n = 4 per group). Expression of genes involved in fatty acid metabolism (E) and mitochondrial biosynthesis (H) in renal tubules after dapagliflozin treatment ( n = 6 per group). (F,I) Paraffin-embedded renal sections were stained with CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (G,J) Histopathological assessment of CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. DM + Saline group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. DM + Dapa group.

    Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

    Techniques: Expressing, Staining

    High salt attenuates gene and protein expression in HG-cultured HK-2 treated with dapagliflozin. Expression of fibrosis-related genes (A) and Na+/K + -ATPase-related genes (B) in HG-treated HK-2 after exposure to different concentrations of dapagliflozin. n = 4 per group. (C,D) Expression of SGLT2, FN and ATP1A1 proteins in HG-treated HK-2 after exposure to 5uM and 10uM dapagliflozin. n = 3 per group. (E) Expression of fatty acid metabolism-related genes. n = 3 per group. (F,G) Expression of FN, ATP1A1 and CPT1A proteins in HK-2 co-cultured with HG, 15 mM NaCl and 5 uM dapagliflozin. n = 3 per group. All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. HG + DMSO group.

    Journal: Frontiers in Pharmacology

    Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

    doi: 10.3389/fphar.2021.741087

    Figure Lengend Snippet: High salt attenuates gene and protein expression in HG-cultured HK-2 treated with dapagliflozin. Expression of fibrosis-related genes (A) and Na+/K + -ATPase-related genes (B) in HG-treated HK-2 after exposure to different concentrations of dapagliflozin. n = 4 per group. (C,D) Expression of SGLT2, FN and ATP1A1 proteins in HG-treated HK-2 after exposure to 5uM and 10uM dapagliflozin. n = 3 per group. (E) Expression of fatty acid metabolism-related genes. n = 3 per group. (F,G) Expression of FN, ATP1A1 and CPT1A proteins in HK-2 co-cultured with HG, 15 mM NaCl and 5 uM dapagliflozin. n = 3 per group. All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. HG + DMSO group.

    Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

    Techniques: Expressing, Cell Culture