Journal: American Journal of Physiology - Renal Physiology

Article Title: Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction

doi: 10.1152/ajprenal.00231.2016

Figure Lengend Snippet: Primer sequences for RT-PCR (mouse)

Article Snippet: Antibodies to NLRP3 were from Novus, caspase-1 from Abcam, ASC from Santa Cruz Biotechnology, p-AKT (ser437), AKT, and IL-1β from Cell Signaling Technology, fibronectin from Sigma, COX2, α-SMA, collagen I, and collagen III from Boster (Wuhan, China).

Techniques: Sequencing

Journal: American Journal of Physiology - Renal Physiology

Article Title: Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction

doi: 10.1152/ajprenal.00231.2016

Figure Lengend Snippet: Analysis of mRNA expression by quantitative real-time PCR for α-SMA (A), fibronectin (B), collagen III (C), and TGF-β1 (D) in obstructed kidneys of mPGES-1 WT and KO mice. α-SMA, α-smooth muscle actin; WT, wild type; KO, knockout. WT-Sham, n = 6; WT-7UUO, n = 7; KO-Sham, n = 6; KO-7UUO, n = 7. *P < 0.05 compared with Sham groups. #P < 0.05 compared with WT-7UUO.

Article Snippet: Antibodies to NLRP3 were from Novus, caspase-1 from Abcam, ASC from Santa Cruz Biotechnology, p-AKT (ser437), AKT, and IL-1β from Cell Signaling Technology, fibronectin from Sigma, COX2, α-SMA, collagen I, and collagen III from Boster (Wuhan, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Knock-Out

Journal: American Journal of Physiology - Renal Physiology

Article Title: Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction

doi: 10.1152/ajprenal.00231.2016

Figure Lengend Snippet: Western blot analysis of α-SMA, fibronectin, and collagen III protein expression in obstructed kidneys of mPGES-1 WT and KO mice. A: semiquantitative immunoblots reacted with anti-α-SMA, fibronectin, and collagen III antibodies. GAPDH was used as internal loading control. Corresponding densitometric analyses of protein expression levels of fibronectin (B) and collagen III (C) corrected by GAPDH (B). WT-Sham, n = 6; WT-7UUO, n = 7; KO-Sham, n = 6; KO-7UUO, n = 7. α-SMA, α-smooth muscle actin. *P < 0.05 compared with Sham groups. §P < 0.05 compared with WT-Sham. #P < 0.05 compared with WT-7UUO.

Article Snippet: Antibodies to NLRP3 were from Novus, caspase-1 from Abcam, ASC from Santa Cruz Biotechnology, p-AKT (ser437), AKT, and IL-1β from Cell Signaling Technology, fibronectin from Sigma, COX2, α-SMA, collagen I, and collagen III from Boster (Wuhan, China).

Techniques: Western Blot, Expressing

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet:

Article Snippet: TotalSeq-A0374 anti-human CD98 (MEM-108) , BioLegend , 315605; RRID: AB_2750369.

Techniques: Purification, Recombinant, Staining, Expressing, Software, Microscopy

Journal: Frontiers in Pharmacology

Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

doi: 10.3389/fphar.2021.741087

Figure Lengend Snippet: High-salt diet alters SGLT2 and Na+/K + -ATPase expression in renal tubules of DKD mice. (A) Expression of SGLT2 gene in renal tubules ( n = 6 per group). (B) Paraffin-embedded renal sections were stained with SGLT2, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (C) Histopathological assessment of SGLT2, ATP1A1 and ATP1B1 proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; ## p < 0.01 and ### p < 0.001 vs. DM group.

Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

Techniques: Expressing, Staining

Journal: Frontiers in Pharmacology

Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

doi: 10.3389/fphar.2021.741087

Figure Lengend Snippet: High salt alters SGLT2 and Na+/K + -ATPase expression in HG-treated HK-2. (A) Intracellular glucose concentration. (B,C) Expression of SGLT2 in HK-2 in HK-2 cultured with HG and 15 mM NaCl. (D) Expression of NKAIN4, ATP1A1, ATP1B1 and ATP1B3 genes in HK-2 after exposure to HG and different concentrations of NaCl. (E,F) Expression of ATP1A1 protein in HK-2 with HG and 15 mM NaCl cultured. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; # p < 0.05 and ## p < 0.01 vs. HG group. (G) Expression of NKAIN4 and fatty acid metabolism related genes were detected in HK-2 treated with Digoxin. * p < 0.05 and *** p < 0.001 vs. DM + DMSO group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. HG + NaCl + DMSO group. All data are mean ± SEM, n = 3 per group.

Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

Techniques: Expressing, Concentration Assay, Cell Culture

Journal: Frontiers in Pharmacology

Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

doi: 10.3389/fphar.2021.741087

Figure Lengend Snippet: | High-salt diet attenuates gene and protein expression in diabetic mice treated with dapagliflozin. (A) The detection of ACR ( n = 6 per group). (B) Expression of α Sma, Col-1 and Slc5a2 genes in renal tubules after dapagliflozin treatment ( n = 6 per group). (C) Paraffin-embedded renal sections were stained with FN and SGLT2 antibodies (magnification, 400×, bar = 20 μm). (D) Histopathological assessment of FN and SGLT2 proteins ( n = 4 per group). Expression of genes involved in fatty acid metabolism (E) and mitochondrial biosynthesis (H) in renal tubules after dapagliflozin treatment ( n = 6 per group). (F,I) Paraffin-embedded renal sections were stained with CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (G,J) Histopathological assessment of CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. DM + Saline group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. DM + Dapa group.

Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

Techniques: Expressing, Staining

Journal: Frontiers in Pharmacology

Article Title: High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease

doi: 10.3389/fphar.2021.741087

Figure Lengend Snippet: High salt attenuates gene and protein expression in HG-cultured HK-2 treated with dapagliflozin. Expression of fibrosis-related genes (A) and Na+/K + -ATPase-related genes (B) in HG-treated HK-2 after exposure to different concentrations of dapagliflozin. n = 4 per group. (C,D) Expression of SGLT2, FN and ATP1A1 proteins in HG-treated HK-2 after exposure to 5uM and 10uM dapagliflozin. n = 3 per group. (E) Expression of fatty acid metabolism-related genes. n = 3 per group. (F,G) Expression of FN, ATP1A1 and CPT1A proteins in HK-2 co-cultured with HG, 15 mM NaCl and 5 uM dapagliflozin. n = 3 per group. All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. HG + DMSO group.

Article Snippet: For immunohistochemical analysis, the sections were incubated with primary antibodies at 4°C overnight, including anti-FN (1:300; F3648; Sigma, United States), anti-CPT1A (1:800; 15184-1-AP; proteintech, United States), anti-ACOX1 (1:900; 10957-1-AP; proteintech), anti-FABP4 (1:50; 12802-1-AP; proteintech), anti-FASN (1:350; 10624-2-AP; proteintech), anti-SGLT2 (1:2,000; A03748-1; BOSTER, China), anti-ATP1A1 (1:200; 14418-1-AP; proteintech) and anti-ATP1B1 (1:50; 15192-1-AP; proteintech).

Techniques: Expressing, Cell Culture