A-520 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Array BioPharma arry 520
    <t>ARRY-520</t> induces p53-independent cell cycle block and cell death. OCI-AML3vec and OCI-AML3p53shRNA cells were treated with ARRY-520. p53 and caspase-3 activation were determined by western blot at various time points (A), cell death by annexin V staining at 48 hours (B), and cell cycle distribution by PI staining at 24 hours (C).
    Arry 520, supplied by Array BioPharma, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arry 520/product/Array BioPharma
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    arry 520 - by Bioz Stars, 2022-10
    88/100 stars
      Buy from Supplier

    99
    Millipore donkey serum
    <t>ARRY-520</t> induces p53-independent cell cycle block and cell death. OCI-AML3vec and OCI-AML3p53shRNA cells were treated with ARRY-520. p53 and caspase-3 activation were determined by western blot at various time points (A), cell death by annexin V staining at 48 hours (B), and cell cycle distribution by PI staining at 24 hours (C).
    Donkey Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey serum/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey serum - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    99
    BioTek Instruments microplate reader
    Characterizations of electrospun fibers with different CNT amounts. A) Gross images and B) representative SEM micrographs of PLA/CNT composite fibers. C) TGA thermograms under a nitrogen atmosphere and D) absorbance at 650 nm measured by <t>microplate</t> reader of electrospun PLA/CNT fibers with different CNT concentrations. Results are presented as means ± standard deviations ( n = 3; * p
    Microplate Reader, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplate reader/product/BioTek Instruments
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microplate reader - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    96
    Thermo Fisher chip assay kit
    Characterizations of electrospun fibers with different CNT amounts. A) Gross images and B) representative SEM micrographs of PLA/CNT composite fibers. C) TGA thermograms under a nitrogen atmosphere and D) absorbance at 650 nm measured by <t>microplate</t> reader of electrospun PLA/CNT fibers with different CNT concentrations. Results are presented as means ± standard deviations ( n = 3; * p
    Chip Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip assay kit/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chip assay kit - by Bioz Stars, 2022-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    ARRY-520 induces p53-independent cell cycle block and cell death. OCI-AML3vec and OCI-AML3p53shRNA cells were treated with ARRY-520. p53 and caspase-3 activation were determined by western blot at various time points (A), cell death by annexin V staining at 48 hours (B), and cell cycle distribution by PI staining at 24 hours (C).

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520 induces p53-independent cell cycle block and cell death. OCI-AML3vec and OCI-AML3p53shRNA cells were treated with ARRY-520. p53 and caspase-3 activation were determined by western blot at various time points (A), cell death by annexin V staining at 48 hours (B), and cell cycle distribution by PI staining at 24 hours (C).

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques: Blocking Assay, Activation Assay, Western Blot, Staining

    ARRY-520 significantly inhibits tumor growth in HL60 (A) and MV4-11 (B) xenografts of SCID mice. ↑, treatment days (days 1, 5, and 9). ↓, retreatment days (days 28, 53, and 67).

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520 significantly inhibits tumor growth in HL60 (A) and MV4-11 (B) xenografts of SCID mice. ↑, treatment days (days 1, 5, and 9). ↓, retreatment days (days 28, 53, and 67).

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques: Mouse Assay

    ARRY-520-induced cell death is mediated via the mitochondrial apoptotic pathway. HL-60 and HL-60Bcl-2 cells were treated with ARRY-520. Cell cycle distribution was determined at 24 hours by PI staining, changes in MMP at 24 hours by CMXRos and MitoTracker Green staining, and apoptosis by annexin V/7-AAD staining (A). HL-60 and HL-60Bcl-2 cells were treated with ARRY-520, ABT-737, or both and cell death was determined at 24 hours by annexin V/7-AAD staining (B). HL-60 cells were treated with 10 nM ARRY-520 for various time points, and Bim and caspase-3 levels were determined by western blot analysis (C). ABT, ABT-737; AR, ARRY-520.

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520-induced cell death is mediated via the mitochondrial apoptotic pathway. HL-60 and HL-60Bcl-2 cells were treated with ARRY-520. Cell cycle distribution was determined at 24 hours by PI staining, changes in MMP at 24 hours by CMXRos and MitoTracker Green staining, and apoptosis by annexin V/7-AAD staining (A). HL-60 and HL-60Bcl-2 cells were treated with ARRY-520, ABT-737, or both and cell death was determined at 24 hours by annexin V/7-AAD staining (B). HL-60 cells were treated with 10 nM ARRY-520 for various time points, and Bim and caspase-3 levels were determined by western blot analysis (C). ABT, ABT-737; AR, ARRY-520.

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques: Staining, Western Blot

    ARRY-520 greatly inhibits the colony formation capacity of BM from patients with AML. BM samples from 5 AML patients (A) and blood cells from 3 normal samples obtained by apheresis (B) were treated with ARRY-520, and CFU was determined. CFU, colony forming units.

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520 greatly inhibits the colony formation capacity of BM from patients with AML. BM samples from 5 AML patients (A) and blood cells from 3 normal samples obtained by apheresis (B) were treated with ARRY-520, and CFU was determined. CFU, colony forming units.

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques:

    ARRY-520 induces dose- and time-dependent cell death in acute leukemic cells. Cells were treated with ARRY-520 and cell death was determined at 24 and 48 hours by annexin V/7-AAD staining (A). HL-60 cells were transfected with either KSP ASO or NSO for 24 hours and then treated with ARRY-520 for 48 hours. Cell death was determined by annexin V staining (B).

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520 induces dose- and time-dependent cell death in acute leukemic cells. Cells were treated with ARRY-520 and cell death was determined at 24 and 48 hours by annexin V/7-AAD staining (A). HL-60 cells were transfected with either KSP ASO or NSO for 24 hours and then treated with ARRY-520 for 48 hours. Cell death was determined by annexin V staining (B).

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques: Staining, Transfection, Allele-specific Oligonucleotide

    ARRY-520 induces G2M cell cycle block prior to cell death. ARRY-520 (1 nM) induced significant G2M cell cycle block in OCI-AML3 cells at 24 hours (A). Cell cycle distribution at different time points in OCI-AML3 cells treated with ARRY-520 (B). Annexin V positivity at different time points in OCI-AML3 cells treated with ARRY-520 (C).

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520 induces G2M cell cycle block prior to cell death. ARRY-520 (1 nM) induced significant G2M cell cycle block in OCI-AML3 cells at 24 hours (A). Cell cycle distribution at different time points in OCI-AML3 cells treated with ARRY-520 (B). Annexin V positivity at different time points in OCI-AML3 cells treated with ARRY-520 (C).

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques: Blocking Assay

    ARRY-520 induces primarily G2M cell death. OCI-AML3 cells were treated with 1.1 nM ARRY-520. Cell cycle distribution/cell death was assessed by TUNEL assay at 24 hours.

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520 induces primarily G2M cell death. OCI-AML3 cells were treated with 1.1 nM ARRY-520. Cell cycle distribution/cell death was assessed by TUNEL assay at 24 hours.

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques: TUNEL Assay

    ARRY-520-induced cell death is independent of XIAP levels and activation of the extrinsic apoptotic pathway. XIAP-overexpressing U937 cells (U937XIAP) (A) and caspase-8-muated Jurkat cells (JurkatI9.2) (B) and their respective control cells (U937neo and Jurkat) were treated with ARRY-520, and cell death was determined by annexin V staining at 24 hours.

    Journal: Leukemia

    Article Title: Inhibition of KSP by ARRY-520 Induces Cell Cycle Block and Cell Death via the Mitochondrial Pathway in AML Cells

    doi: 10.1038/leu.2009.101

    Figure Lengend Snippet: ARRY-520-induced cell death is independent of XIAP levels and activation of the extrinsic apoptotic pathway. XIAP-overexpressing U937 cells (U937XIAP) (A) and caspase-8-muated Jurkat cells (JurkatI9.2) (B) and their respective control cells (U937neo and Jurkat) were treated with ARRY-520, and cell death was determined by annexin V staining at 24 hours.

    Article Snippet: To test whether XIAP, a potent caspase inhibitor that suppresses post-mitochondrial apoptosis, affects cell sensitivity and whether the activation of the extrinsic pathway is required for ARRY-520 action, we treated XIAP-overexpressing U937 cells (U937XIAP) and caspase-8-mutated Jurkat cells (JurkatI9.2) and their respective control cells (U937neo and Jurkat) with ARRY-520 and found that ARRY-520 had similar efficacy in U937neo and U937XIAP ( ) and in JurkatI9.2 and Jurkat cells , regardless of the XIAP levels and caspase-8 status.

    Techniques: Activation Assay, Staining

    Characterizations of electrospun fibers with different CNT amounts. A) Gross images and B) representative SEM micrographs of PLA/CNT composite fibers. C) TGA thermograms under a nitrogen atmosphere and D) absorbance at 650 nm measured by microplate reader of electrospun PLA/CNT fibers with different CNT concentrations. Results are presented as means ± standard deviations ( n = 3; * p

    Journal: Small (Weinheim an der Bergstrasse, Germany)

    Article Title: Porous Electrospun Fibers with Self-Sealing Functionality: An Enabling Strategy for Trapping Biomacromolecules

    doi: 10.1002/smll.201701949

    Figure Lengend Snippet: Characterizations of electrospun fibers with different CNT amounts. A) Gross images and B) representative SEM micrographs of PLA/CNT composite fibers. C) TGA thermograms under a nitrogen atmosphere and D) absorbance at 650 nm measured by microplate reader of electrospun PLA/CNT fibers with different CNT concentrations. Results are presented as means ± standard deviations ( n = 3; * p

    Article Snippet: The concentrations of FITC-Dex, TMR-BSA, and FLU-DNA were determined by measuring the fluorescence intensity with excitation/emission wavelength at 495/520, 541/572, and 495/520 nm, respectively, using a microplate reader.

    Techniques: Proximity Ligation Assay