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  • 96
    Thermo Fisher dynamo cdna synthesis kit
    Dynamo Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynamo cdna synthesis kit/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dynamo cdna synthesis kit - by Bioz Stars, 2022-08
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    86
    Miltenyi Biotec lineage cell depletion kit mouse
    Lineage Cell Depletion Kit Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lineage cell depletion kit mouse/product/Miltenyi Biotec
    Average 86 stars, based on 1 article reviews
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    lineage cell depletion kit mouse - by Bioz Stars, 2022-08
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    99
    Miltenyi Biotec mouse lineage cell depletion kit
    Mouse Lineage Cell Depletion Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lineage cell depletion kit/product/Miltenyi Biotec
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    mouse lineage cell depletion kit - by Bioz Stars, 2022-08
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    89
    Rockland Immunochemicals mad2b
    Activation of AMPK prevents <t>MAD2B</t> expression under high glucose. Cortical neurons were treated with 50 mM glucose for 24 hrs and then incubated with metformin (or AICAR) for different time‐points. ( A – C ) Western blot analysis showing the effects of metformin and AICAR on the expression of MAD2B under 50 mM glucose. ( D ) Summarized data. n = 5, * P
    Mad2b, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mad2b/product/Rockland Immunochemicals
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    mad2b - by Bioz Stars, 2022-08
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    Image Search Results


    Activation of AMPK prevents MAD2B expression under high glucose. Cortical neurons were treated with 50 mM glucose for 24 hrs and then incubated with metformin (or AICAR) for different time‐points. ( A – C ) Western blot analysis showing the effects of metformin and AICAR on the expression of MAD2B under 50 mM glucose. ( D ) Summarized data. n = 5, * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

    doi: 10.1111/jcmm.13046

    Figure Lengend Snippet: Activation of AMPK prevents MAD2B expression under high glucose. Cortical neurons were treated with 50 mM glucose for 24 hrs and then incubated with metformin (or AICAR) for different time‐points. ( A – C ) Western blot analysis showing the effects of metformin and AICAR on the expression of MAD2B under 50 mM glucose. ( D ) Summarized data. n = 5, * P

    Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

    Techniques: Activation Assay, Expressing, Incubation, Western Blot

    Expression of MAD2B related with the phosphorylation of AMPK. ( A ) Neurons cultured for at least 6 days were stained with NeuN and Hoechst showing the purity of primary neuronal cells. ( B ) Western blot analysis of the phosphorylation levels of AMPK and MAD2B protein expression from untreated, 50 mM glucose‐treated, AICAR‐treated and metformin‐treated cortical neurons. ( C and D ) Summarized data showing the band intensities for p‐AMPK and MAD2B, respectively. ( E ) Western blot analysis showing the effects of AICAR and metformin on the basal level of MAD2B in neuronal cells. Ctrl: control; Vehl: vehicle; AIC: AICAR; Met: metformin. n = 5, * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

    doi: 10.1111/jcmm.13046

    Figure Lengend Snippet: Expression of MAD2B related with the phosphorylation of AMPK. ( A ) Neurons cultured for at least 6 days were stained with NeuN and Hoechst showing the purity of primary neuronal cells. ( B ) Western blot analysis of the phosphorylation levels of AMPK and MAD2B protein expression from untreated, 50 mM glucose‐treated, AICAR‐treated and metformin‐treated cortical neurons. ( C and D ) Summarized data showing the band intensities for p‐AMPK and MAD2B, respectively. ( E ) Western blot analysis showing the effects of AICAR and metformin on the basal level of MAD2B in neuronal cells. Ctrl: control; Vehl: vehicle; AIC: AICAR; Met: metformin. n = 5, * P

    Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

    Techniques: Expressing, Cell Culture, Staining, Western Blot

    Metformin promoted the degradation of MAD2B through ubiquitylation. Cortical neurons were pretreated with CHX or Met for 30 min., then treated with high glucose for 4 hrs. ( A ) Western blot analysis showed the effects of high glucose on the ubiquitylation levels of MAD2B under high glucose. ( B ) Western blot analysis showed the effects of metformin on ubiquitylation levels of MAD2B. C: control; G: glucose; Met: metformin.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

    doi: 10.1111/jcmm.13046

    Figure Lengend Snippet: Metformin promoted the degradation of MAD2B through ubiquitylation. Cortical neurons were pretreated with CHX or Met for 30 min., then treated with high glucose for 4 hrs. ( A ) Western blot analysis showed the effects of high glucose on the ubiquitylation levels of MAD2B under high glucose. ( B ) Western blot analysis showed the effects of metformin on ubiquitylation levels of MAD2B. C: control; G: glucose; Met: metformin.

    Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

    Techniques: Western Blot

    AMPK promoted the degradation of MAD2B. Cortical neurons were treated with metformin (or AICAR) for 30 min. and then treated with glucose for different time‐points. ( A , C , E ) Western blot analysis of the expression of MAD2B. ( B , D and F ) Summarized data showing the band intensities for MAD2B. Ctrl control; Vehl vehicle; CHX: cycloheximide; Met: metformin. n = 5, * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

    doi: 10.1111/jcmm.13046

    Figure Lengend Snippet: AMPK promoted the degradation of MAD2B. Cortical neurons were treated with metformin (or AICAR) for 30 min. and then treated with glucose for different time‐points. ( A , C , E ) Western blot analysis of the expression of MAD2B. ( B , D and F ) Summarized data showing the band intensities for MAD2B. Ctrl control; Vehl vehicle; CHX: cycloheximide; Met: metformin. n = 5, * P

    Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

    Techniques: Western Blot, Expressing

    Degradation of MAD2B was inhibited by high glucose in cortical neurons. ( A and B ) Represent gels and summarized data showing the effects of transcription inhibitor actinomycin D on the expression of MAD2B. Neurons were pretreated with actinomycin D (1.5 μM) for 30 min., and then incubated with 50 mM glucose for 12 hrs. * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

    doi: 10.1111/jcmm.13046

    Figure Lengend Snippet: Degradation of MAD2B was inhibited by high glucose in cortical neurons. ( A and B ) Represent gels and summarized data showing the effects of transcription inhibitor actinomycin D on the expression of MAD2B. Neurons were pretreated with actinomycin D (1.5 μM) for 30 min., and then incubated with 50 mM glucose for 12 hrs. * P

    Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

    Techniques: Expressing, Incubation

    Degradation of MAD2B is dependent on ubiquitin–proteasome pathway. Cortical neurons were pretreated with 3‐MA (5 mmol/l), NH4Cl (5 mmol/l) or MG‐132 (20 μM) under normal or high glucose‐treated condition. ( A and C ) Western blot analysis of the effects of 3‐MA, NH4Cl and MG‐132 on the expression of MAD2B under normal culture medium and 50 mM glucose, respectively. ( B and D ) Summarized data showing the band intensities for MAD2B. Ctrl control; Vehl vehicle; CHX: cycloheximide. n = 5, * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

    doi: 10.1111/jcmm.13046

    Figure Lengend Snippet: Degradation of MAD2B is dependent on ubiquitin–proteasome pathway. Cortical neurons were pretreated with 3‐MA (5 mmol/l), NH4Cl (5 mmol/l) or MG‐132 (20 μM) under normal or high glucose‐treated condition. ( A and C ) Western blot analysis of the effects of 3‐MA, NH4Cl and MG‐132 on the expression of MAD2B under normal culture medium and 50 mM glucose, respectively. ( B and D ) Summarized data showing the band intensities for MAD2B. Ctrl control; Vehl vehicle; CHX: cycloheximide. n = 5, * P

    Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

    Techniques: Western Blot, Expressing