Journal: Journal of Cellular and Molecular Medicine

Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

doi: 10.1111/jcmm.13046

Figure Lengend Snippet: Activation of AMPK prevents MAD2B expression under high glucose. Cortical neurons were treated with 50 mM glucose for 24 hrs and then incubated with metformin (or AICAR) for different time‐points. ( A – C ) Western blot analysis showing the effects of metformin and AICAR on the expression of MAD2B under 50 mM glucose. ( D ) Summarized data. n = 5, * P

Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

Techniques: Activation Assay, Expressing, Incubation, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

doi: 10.1111/jcmm.13046

Figure Lengend Snippet: Expression of MAD2B related with the phosphorylation of AMPK. ( A ) Neurons cultured for at least 6 days were stained with NeuN and Hoechst showing the purity of primary neuronal cells. ( B ) Western blot analysis of the phosphorylation levels of AMPK and MAD2B protein expression from untreated, 50 mM glucose‐treated, AICAR‐treated and metformin‐treated cortical neurons. ( C and D ) Summarized data showing the band intensities for p‐AMPK and MAD2B, respectively. ( E ) Western blot analysis showing the effects of AICAR and metformin on the basal level of MAD2B in neuronal cells. Ctrl: control; Vehl: vehicle; AIC: AICAR; Met: metformin. n = 5, * P

Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

Techniques: Expressing, Cell Culture, Staining, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

doi: 10.1111/jcmm.13046

Figure Lengend Snippet: Metformin promoted the degradation of MAD2B through ubiquitylation. Cortical neurons were pretreated with CHX or Met for 30 min., then treated with high glucose for 4 hrs. ( A ) Western blot analysis showed the effects of high glucose on the ubiquitylation levels of MAD2B under high glucose. ( B ) Western blot analysis showed the effects of metformin on ubiquitylation levels of MAD2B. C: control; G: glucose; Met: metformin.

Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

Techniques: Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

doi: 10.1111/jcmm.13046

Figure Lengend Snippet: AMPK promoted the degradation of MAD2B. Cortical neurons were treated with metformin (or AICAR) for 30 min. and then treated with glucose for different time‐points. ( A , C , E ) Western blot analysis of the expression of MAD2B. ( B , D and F ) Summarized data showing the band intensities for MAD2B. Ctrl control; Vehl vehicle; CHX: cycloheximide; Met: metformin. n = 5, * P

Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

Techniques: Western Blot, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

doi: 10.1111/jcmm.13046

Figure Lengend Snippet: Degradation of MAD2B was inhibited by high glucose in cortical neurons. ( A and B ) Represent gels and summarized data showing the effects of transcription inhibitor actinomycin D on the expression of MAD2B. Neurons were pretreated with actinomycin D (1.5 μM) for 30 min., and then incubated with 50 mM glucose for 12 hrs. * P

Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

Techniques: Expressing, Incubation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells

doi: 10.1111/jcmm.13046

Figure Lengend Snippet: Degradation of MAD2B is dependent on ubiquitin–proteasome pathway. Cortical neurons were pretreated with 3‐MA (5 mmol/l), NH4Cl (5 mmol/l) or MG‐132 (20 μM) under normal or high glucose‐treated condition. ( A and C ) Western blot analysis of the effects of 3‐MA, NH4Cl and MG‐132 on the expression of MAD2B under normal culture medium and 50 mM glucose, respectively. ( B and D ) Summarized data showing the band intensities for MAD2B. Ctrl control; Vehl vehicle; CHX: cycloheximide. n = 5, * P

Article Snippet: Then, we elucidated the degradation mechanism of MAD2B under high glucose.

Techniques: Western Blot, Expressing