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  • 95
    Enzo Biochem p62
    CSL Protein Down-Modulation Requires Autophagy (A) Pharmacologic inhibition of autophagy blocks CSL down-modulation. Immunoblot analysis was performed for two HDF strains treated as indicated in Figure 1A for 24 hr and further incubated with 5 mM 3-methyladenine (3MA) for 8 hr to inhibit autophagy, in parallel with vehicle alone (DMEM). The blot was sequentially probed with antibodies against CSL, <t>p62,</t> and β-ACTIN. (B and C) Targeting of autophagy-essential gene ATG5 blocks CSL down-modulation. Immunoblot analysis was performed for two HDFs plus/minus shRNA-mediated ATG5 gene silencing (B) or for mouse embryo fibroblasts (MEFs) plus/minus Atg5 gene disruption (+/+ and /) ( Kuma et al., 2004 ) (C) that were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against CSL, ATG5, and ACTIN as equal loading.
    P62, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p62/product/Enzo Biochem
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p62 - by Bioz Stars, 2022-09
    95/100 stars
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    99
    Illumina Inc illumina caninehd beadchip
    CSL Protein Down-Modulation Requires Autophagy (A) Pharmacologic inhibition of autophagy blocks CSL down-modulation. Immunoblot analysis was performed for two HDF strains treated as indicated in Figure 1A for 24 hr and further incubated with 5 mM 3-methyladenine (3MA) for 8 hr to inhibit autophagy, in parallel with vehicle alone (DMEM). The blot was sequentially probed with antibodies against CSL, <t>p62,</t> and β-ACTIN. (B and C) Targeting of autophagy-essential gene ATG5 blocks CSL down-modulation. Immunoblot analysis was performed for two HDFs plus/minus shRNA-mediated ATG5 gene silencing (B) or for mouse embryo fibroblasts (MEFs) plus/minus Atg5 gene disruption (+/+ and /) ( Kuma et al., 2004 ) (C) that were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against CSL, ATG5, and ACTIN as equal loading.
    Illumina Caninehd Beadchip, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina caninehd beadchip/product/Illumina Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    illumina caninehd beadchip - by Bioz Stars, 2022-09
    99/100 stars
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    88
    Bio-Rad 4 4 0 software
    CSL Protein Down-Modulation Requires Autophagy (A) Pharmacologic inhibition of autophagy blocks CSL down-modulation. Immunoblot analysis was performed for two HDF strains treated as indicated in Figure 1A for 24 hr and further incubated with 5 mM 3-methyladenine (3MA) for 8 hr to inhibit autophagy, in parallel with vehicle alone (DMEM). The blot was sequentially probed with antibodies against CSL, <t>p62,</t> and β-ACTIN. (B and C) Targeting of autophagy-essential gene ATG5 blocks CSL down-modulation. Immunoblot analysis was performed for two HDFs plus/minus shRNA-mediated ATG5 gene silencing (B) or for mouse embryo fibroblasts (MEFs) plus/minus Atg5 gene disruption (+/+ and /) ( Kuma et al., 2004 ) (C) that were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against CSL, ATG5, and ACTIN as equal loading.
    4 4 0 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4 4 0 software/product/Bio-Rad
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    4 4 0 software - by Bioz Stars, 2022-09
    88/100 stars
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    88
    Exeter Analytical ce 440 elemental analyzer
    CSL Protein Down-Modulation Requires Autophagy (A) Pharmacologic inhibition of autophagy blocks CSL down-modulation. Immunoblot analysis was performed for two HDF strains treated as indicated in Figure 1A for 24 hr and further incubated with 5 mM 3-methyladenine (3MA) for 8 hr to inhibit autophagy, in parallel with vehicle alone (DMEM). The blot was sequentially probed with antibodies against CSL, <t>p62,</t> and β-ACTIN. (B and C) Targeting of autophagy-essential gene ATG5 blocks CSL down-modulation. Immunoblot analysis was performed for two HDFs plus/minus shRNA-mediated ATG5 gene silencing (B) or for mouse embryo fibroblasts (MEFs) plus/minus Atg5 gene disruption (+/+ and /) ( Kuma et al., 2004 ) (C) that were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against CSL, ATG5, and ACTIN as equal loading.
    Ce 440 Elemental Analyzer, supplied by Exeter Analytical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ce 440 elemental analyzer/product/Exeter Analytical
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ce 440 elemental analyzer - by Bioz Stars, 2022-09
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    Image Search Results


    CSL Protein Down-Modulation Requires Autophagy (A) Pharmacologic inhibition of autophagy blocks CSL down-modulation. Immunoblot analysis was performed for two HDF strains treated as indicated in Figure 1A for 24 hr and further incubated with 5 mM 3-methyladenine (3MA) for 8 hr to inhibit autophagy, in parallel with vehicle alone (DMEM). The blot was sequentially probed with antibodies against CSL, p62, and β-ACTIN. (B and C) Targeting of autophagy-essential gene ATG5 blocks CSL down-modulation. Immunoblot analysis was performed for two HDFs plus/minus shRNA-mediated ATG5 gene silencing (B) or for mouse embryo fibroblasts (MEFs) plus/minus Atg5 gene disruption (+/+ and /) ( Kuma et al., 2004 ) (C) that were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against CSL, ATG5, and ACTIN as equal loading.

    Journal: Cell reports

    Article Title: Autophagy Controls CSL/RBPJκ Stability through a p62/SQSTM1-Dependent Mechanism

    doi: 10.1016/j.celrep.2018.08.043

    Figure Lengend Snippet: CSL Protein Down-Modulation Requires Autophagy (A) Pharmacologic inhibition of autophagy blocks CSL down-modulation. Immunoblot analysis was performed for two HDF strains treated as indicated in Figure 1A for 24 hr and further incubated with 5 mM 3-methyladenine (3MA) for 8 hr to inhibit autophagy, in parallel with vehicle alone (DMEM). The blot was sequentially probed with antibodies against CSL, p62, and β-ACTIN. (B and C) Targeting of autophagy-essential gene ATG5 blocks CSL down-modulation. Immunoblot analysis was performed for two HDFs plus/minus shRNA-mediated ATG5 gene silencing (B) or for mouse embryo fibroblasts (MEFs) plus/minus Atg5 gene disruption (+/+ and /) ( Kuma et al., 2004 ) (C) that were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against CSL, ATG5, and ACTIN as equal loading.

    Article Snippet: For the recombinant protein interaction, 500 ng of recombinant CSL (Origene) and p62 (Enzo) were diluted in 500 ml PBS and incubated 2h at 4C on a rotor.

    Techniques: Inhibition, Incubation, shRNA

    CSL Down-Modulation Is Linked to Higher Autophagy in CAFs (A) p62 and CSL levels are concomitantly downmodulated in CAFs. Left: immunoblot with anti-p62, anti-CSL, and anti-ACTIN antibodies in nonmatched HDF and CAF strains. Right: immunoblot quantifications, n (HDF) = 2, n (CAF) = 4; *p

    Journal: Cell reports

    Article Title: Autophagy Controls CSL/RBPJκ Stability through a p62/SQSTM1-Dependent Mechanism

    doi: 10.1016/j.celrep.2018.08.043

    Figure Lengend Snippet: CSL Down-Modulation Is Linked to Higher Autophagy in CAFs (A) p62 and CSL levels are concomitantly downmodulated in CAFs. Left: immunoblot with anti-p62, anti-CSL, and anti-ACTIN antibodies in nonmatched HDF and CAF strains. Right: immunoblot quantifications, n (HDF) = 2, n (CAF) = 4; *p

    Article Snippet: For the recombinant protein interaction, 500 ng of recombinant CSL (Origene) and p62 (Enzo) were diluted in 500 ml PBS and incubated 2h at 4C on a rotor.

    Techniques:

    Endogenous p62 and CSL Proteins Directly Interact in HDFs (A and B) Proximity ligation assays (PLAs) for in situ detection of CSL and p62 association in HDFs. Assays were carried out in the presence or absence of primary antibodies (A) or with HDFs plus/minus siRNA-mediated CSL gene silencing (B) as controls of specificity. Confocal microscopy was used to examine red fluorescence PLA puncta with concomitant DAPI staining of nuclei (blue). The white frames indicate the enlarged areas to better show PLA red puncta. Shown are representative images and quantification of the average number of puncta per cell, counting at least 30 cells in four fields per conditions. p ≤ 0.05, two-tailed unpaired t test. Scale bars, 10 and 1 μm for enlargements. Additional PLA images of p62 and CSL interaction are provided in Figures S1A and S1B . (C) Endogenous p62 and CSL proteins interact. Immunoprecipitates from HDFs (strain SAN4) with anti-CSL antibodies and non-immune immunoglobulin Gs (IgGs) were analyzed, together with total inputs by sequential immunoblotting with antip62 and CSL antibodies. IgG light chains (LCs) are indicated. A similar immunoprecipitation in another HDF strain (SAN3) is shown in Figure S1C . (D) p62 and CSL proteins directly interact. Recombinant CSL and p62 proteins were admixed and incubated for 2 hr before immunoprecipitation with either anti-CSL or anti-p62 antibodies or nonimmune IgGs, followed by immunoblot analysis with anti-p62 antibodies (green) and anti-CSL antibodies (red). IgG heavy chains (HCs) are indicated. (E) p62 interaction with CSL requires p62 C-terminal region. Immunoblot analysis showed HEK293 transfected for 48 hr with expression vectors for CSL-FLAG, GFP-p62, and a combination of CSLFLAG and GFP-p62 wild-type (WT) or GFP-p62 constructs with deletions of the N-terminal PB1 region, LIR, and C-terminal UBA region (as indicated in the scheme) ( Bjørkøy et al., 2005 ). Total lysates were immunoprecipitated with anti-FLAG antibodies, and blots were probed sequentially with anti-GFP and anti-FLAG antibodies. The input totals were analyzed by parallel immunoblotting as control for the level of expression.

    Journal: Cell reports

    Article Title: Autophagy Controls CSL/RBPJκ Stability through a p62/SQSTM1-Dependent Mechanism

    doi: 10.1016/j.celrep.2018.08.043

    Figure Lengend Snippet: Endogenous p62 and CSL Proteins Directly Interact in HDFs (A and B) Proximity ligation assays (PLAs) for in situ detection of CSL and p62 association in HDFs. Assays were carried out in the presence or absence of primary antibodies (A) or with HDFs plus/minus siRNA-mediated CSL gene silencing (B) as controls of specificity. Confocal microscopy was used to examine red fluorescence PLA puncta with concomitant DAPI staining of nuclei (blue). The white frames indicate the enlarged areas to better show PLA red puncta. Shown are representative images and quantification of the average number of puncta per cell, counting at least 30 cells in four fields per conditions. p ≤ 0.05, two-tailed unpaired t test. Scale bars, 10 and 1 μm for enlargements. Additional PLA images of p62 and CSL interaction are provided in Figures S1A and S1B . (C) Endogenous p62 and CSL proteins interact. Immunoprecipitates from HDFs (strain SAN4) with anti-CSL antibodies and non-immune immunoglobulin Gs (IgGs) were analyzed, together with total inputs by sequential immunoblotting with antip62 and CSL antibodies. IgG light chains (LCs) are indicated. A similar immunoprecipitation in another HDF strain (SAN3) is shown in Figure S1C . (D) p62 and CSL proteins directly interact. Recombinant CSL and p62 proteins were admixed and incubated for 2 hr before immunoprecipitation with either anti-CSL or anti-p62 antibodies or nonimmune IgGs, followed by immunoblot analysis with anti-p62 antibodies (green) and anti-CSL antibodies (red). IgG heavy chains (HCs) are indicated. (E) p62 interaction with CSL requires p62 C-terminal region. Immunoblot analysis showed HEK293 transfected for 48 hr with expression vectors for CSL-FLAG, GFP-p62, and a combination of CSLFLAG and GFP-p62 wild-type (WT) or GFP-p62 constructs with deletions of the N-terminal PB1 region, LIR, and C-terminal UBA region (as indicated in the scheme) ( Bjørkøy et al., 2005 ). Total lysates were immunoprecipitated with anti-FLAG antibodies, and blots were probed sequentially with anti-GFP and anti-FLAG antibodies. The input totals were analyzed by parallel immunoblotting as control for the level of expression.

    Article Snippet: For the recombinant protein interaction, 500 ng of recombinant CSL (Origene) and p62 (Enzo) were diluted in 500 ml PBS and incubated 2h at 4C on a rotor.

    Techniques: Ligation, In Situ, Confocal Microscopy, Fluorescence, Proximity Ligation Assay, Staining, Cell Counting, Two Tailed Test, Immunoprecipitation, Recombinant, Incubation, Transfection, Expressing, Construct

    Autophagy Down-Modulates CSL Protein through a p62-Dependent Mechanism (A and B) Immunoblot analysis of two HDF strains (A) or MEFs (B) plus/minus p62 silencing by siRNA-mediated (A) or shRNA-mediated (B) RNA interference, either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against p62, CSL, and ACTIN antibodies. (C) Immunoblot analysis with antibodies against p62, CSL, and ACTIN antibodies of MEFs plus/minus p62 gene disruption (−/− and +/+) ( Komatsu et al., 2006 ). Cells were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Immunofluorescence analysis with CSL antibodies and DAPI of p62 +/+ and p62 −/− MEFs treated as in Figure 1A .

    Journal: Cell reports

    Article Title: Autophagy Controls CSL/RBPJκ Stability through a p62/SQSTM1-Dependent Mechanism

    doi: 10.1016/j.celrep.2018.08.043

    Figure Lengend Snippet: Autophagy Down-Modulates CSL Protein through a p62-Dependent Mechanism (A and B) Immunoblot analysis of two HDF strains (A) or MEFs (B) plus/minus p62 silencing by siRNA-mediated (A) or shRNA-mediated (B) RNA interference, either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Blots were sequentially probed with antibodies against p62, CSL, and ACTIN antibodies. (C) Immunoblot analysis with antibodies against p62, CSL, and ACTIN antibodies of MEFs plus/minus p62 gene disruption (−/− and +/+) ( Komatsu et al., 2006 ). Cells were either left untreated (untr.) or treated with various pro-autophagic stimuli as in Figure 1A . Immunofluorescence analysis with CSL antibodies and DAPI of p62 +/+ and p62 −/− MEFs treated as in Figure 1A .

    Article Snippet: For the recombinant protein interaction, 500 ng of recombinant CSL (Origene) and p62 (Enzo) were diluted in 500 ml PBS and incubated 2h at 4C on a rotor.

    Techniques: shRNA, Immunofluorescence