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  • 94
    Thermo Fisher cd31
    Limiting Dilution Analysis to Estimate the Residual Endothelial Potency of CD41SP Cells CD41SP cells were cultured on OP9 cells for 6 days at varying cell concentrations. Endothelial cells were detected by staining with <t>anti-CD31</t> and anti-EYFP antibodies. AA4SP cells were analyzed for comparison. The fraction of negative wells was plotted at each cell concentration. Representative results from two separate experiments are shown.
    Cd31, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher cd31 pecam 1 monoclonal antibody
    Limiting Dilution Analysis to Estimate the Residual Endothelial Potency of CD41SP Cells CD41SP cells were cultured on OP9 cells for 6 days at varying cell concentrations. Endothelial cells were detected by staining with <t>anti-CD31</t> and anti-EYFP antibodies. AA4SP cells were analyzed for comparison. The fraction of negative wells was plotted at each cell concentration. Representative results from two separate experiments are shown.
    Cd31 Pecam 1 Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher cd31 pecam 1 monoclonal antibody 390
    Limiting Dilution Analysis to Estimate the Residual Endothelial Potency of CD41SP Cells CD41SP cells were cultured on OP9 cells for 6 days at varying cell concentrations. Endothelial cells were detected by staining with <t>anti-CD31</t> and anti-EYFP antibodies. AA4SP cells were analyzed for comparison. The fraction of negative wells was plotted at each cell concentration. Representative results from two separate experiments are shown.
    Cd31 Pecam 1 Monoclonal Antibody 390, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Takeda dexlansoprazole mr
    Limiting Dilution Analysis to Estimate the Residual Endothelial Potency of CD41SP Cells CD41SP cells were cultured on OP9 cells for 6 days at varying cell concentrations. Endothelial cells were detected by staining with <t>anti-CD31</t> and anti-EYFP antibodies. AA4SP cells were analyzed for comparison. The fraction of negative wells was plotted at each cell concentration. Representative results from two separate experiments are shown.
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    Image Search Results


    Limiting Dilution Analysis to Estimate the Residual Endothelial Potency of CD41SP Cells CD41SP cells were cultured on OP9 cells for 6 days at varying cell concentrations. Endothelial cells were detected by staining with anti-CD31 and anti-EYFP antibodies. AA4SP cells were analyzed for comparison. The fraction of negative wells was plotted at each cell concentration. Representative results from two separate experiments are shown.

    Journal: Stem Cell Reports

    Article Title: Common Developmental Pathway for Primitive Erythrocytes and Multipotent Hematopoietic Progenitors in Early Mouse Development

    doi: 10.1016/j.stemcr.2013.10.008

    Figure Lengend Snippet: Limiting Dilution Analysis to Estimate the Residual Endothelial Potency of CD41SP Cells CD41SP cells were cultured on OP9 cells for 6 days at varying cell concentrations. Endothelial cells were detected by staining with anti-CD31 and anti-EYFP antibodies. AA4SP cells were analyzed for comparison. The fraction of negative wells was plotted at each cell concentration. Representative results from two separate experiments are shown.

    Article Snippet: Antibodies The following antibodies were used in this study: AA4.1 (eBioscience), CD4 (GK1.5, BioLegend), CD8 (53-6.7, BioLegend), CD11b (M1/70, BioLegend), CD19 (6D5, BioLegend), CD31 (390, eBioscience), CD41 (MWReg30, BD Pharmingen), CD45 (30-F11, eBioscience), c-KIT (2B8, BD Pharmingen), FLK-1 (Avas12α1, eBioscience), γδTCR (UC7-13D5, BioLegend), Gr-1 (RB6-8C5, BioLegend), immunoglobulin (Ig) M (II/41, eBioscience), TER119 (eBioscience), and YFP (rabbit anti-GFP, Invitrogen).

    Techniques: Cell Culture, Staining, Concentration Assay

    Cell Subsets within CD45 − Differentiation Stage (A) Cell subsets present on day 5 of differentiation from Mesp1-cre/Rosa26-eYfp ES cells. The c-KIT expression and May-Grunwald/Giemsa-stained cytospin images of each cell subset are shown. Gray lines in the c-KIT expression panels represent expression in DN cells, which are shown for comparison. Scale bars: 50 μm. (B and C) Differentiation potentials of each cell subset. EYFP-labeled DP and CD41SP cells formed hematopoietic cell colonies on OP9 cells (B), whereas AA4SP cells (anti-EYFP [Alexa Fluor 488; green]) predominantly produced endothelial sheets (anti-CD31 [Cy3; red]) on OP9 cells (C); signals for the two markers were colocalized in identical cells. Scale bars, 200 μm (B) and 400 μm (C). Representative results from three separate experiments are shown. See also Figure S1 .

    Journal: Stem Cell Reports

    Article Title: Common Developmental Pathway for Primitive Erythrocytes and Multipotent Hematopoietic Progenitors in Early Mouse Development

    doi: 10.1016/j.stemcr.2013.10.008

    Figure Lengend Snippet: Cell Subsets within CD45 − Differentiation Stage (A) Cell subsets present on day 5 of differentiation from Mesp1-cre/Rosa26-eYfp ES cells. The c-KIT expression and May-Grunwald/Giemsa-stained cytospin images of each cell subset are shown. Gray lines in the c-KIT expression panels represent expression in DN cells, which are shown for comparison. Scale bars: 50 μm. (B and C) Differentiation potentials of each cell subset. EYFP-labeled DP and CD41SP cells formed hematopoietic cell colonies on OP9 cells (B), whereas AA4SP cells (anti-EYFP [Alexa Fluor 488; green]) predominantly produced endothelial sheets (anti-CD31 [Cy3; red]) on OP9 cells (C); signals for the two markers were colocalized in identical cells. Scale bars, 200 μm (B) and 400 μm (C). Representative results from three separate experiments are shown. See also Figure S1 .

    Article Snippet: Antibodies The following antibodies were used in this study: AA4.1 (eBioscience), CD4 (GK1.5, BioLegend), CD8 (53-6.7, BioLegend), CD11b (M1/70, BioLegend), CD19 (6D5, BioLegend), CD31 (390, eBioscience), CD41 (MWReg30, BD Pharmingen), CD45 (30-F11, eBioscience), c-KIT (2B8, BD Pharmingen), FLK-1 (Avas12α1, eBioscience), γδTCR (UC7-13D5, BioLegend), Gr-1 (RB6-8C5, BioLegend), immunoglobulin (Ig) M (II/41, eBioscience), TER119 (eBioscience), and YFP (rabbit anti-GFP, Invitrogen).

    Techniques: Expressing, Staining, Labeling, Produced