A-390 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher cd31
    OT1-iT cell therapy suppresses the solid tumor growth in mice transplanted with E.G7-OVA cells a Schematic diagram of OT1 engineered iT cells for anti-tumor therapy. Mouse MEF cells were isolated from CD45.2 + C57BL/6 mouse and reprogrammed into iPSC with Oct4, Klf4, and Sox2 retro-viruses. Then a rtTA-TRE-Runx1-Hoxa9-HygroR DNA cassette was inserted into the Rosa26 locus . Next, a CAG-OT1-IRES-GFP-PuroR expression element was inserted into the Hipp11 locus of iR9 -iPSC. OT1- iR9 -iPSC results in the production of CD8 + T cells carrying TCRVα2 and TCRVβ5 (MHC class I-restricted, ovalbumin-specific TCR). OT1- iR9 -iPSC-derived iHEC were induced into iHPC (OT1-iHPC) as described in material and method sections. The iHPC were injected into irradiated (4.5 Gy) Rag1 −/− recipient mice (3 million/mouse, 8-10-week-old C57BL/6 background). E.G7-OVA tumor cell line (C57BL/6 background) were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− (n = 8) by subcutaneous injection (0.2 million/mouse) six weeks after OT1-iHPC transplantation. b TCRVα2 and TCRVβ5 expression in OT1- iR9 -iPSC measured by intracellular staining. The iR9 -iPSC was used as negative control. c Sorting gates of the OT1 - iR9 -iPSC-derived iHEC population at day 11. The cells were enriched by streptavidin-beads recognizing <t>biotin-CD31</t> before sorting. Representative plots from three independent experiments are shown. d Immuno-phenotypes of pre-thymic progenitors in induced hematopoietic progenitor cells from OT1 - iR9 -iPSC-derived iHEC after ten-day maturation. Representative plots from three independent experiments are shown. Lin was defined as CD2 − CD3 − CD4 − CD8 − CD11b − Gr1 − Ter119 − CD19 − NK1.1 − TCRγδ − . pre-thymic progenitors were defined as Lin − c-kit + CD127 + /CD135 + . e TCRVα2 and TCRVβ5 expression of iT cells in PB of Rag1 −/− mice 6 weeks after transplantation of OT1 - iR9 -iPSC-derived iHPC. Three representative mice from three independent experiments were analyzed. f Tumor growth in Rag1 −/− and OT1-iT- Rag1 −/− mice. E.G7-OVA cells were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− mice (n = 8) by subcutaneous injection (0.2 million/mouse). The length and width of the tumors were measured every other day by a caliper, and each tumor size was calculated as length × width (mm 2 ). Mice with tumor size larger than 20 mm at the longest axis were euthanized for ethical consideration. *** P
    Cd31, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd31/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd31 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    86
    Thermo Fisher cd31 pecam 1 monoclonal antibody
    OT1-iT cell therapy suppresses the solid tumor growth in mice transplanted with E.G7-OVA cells a Schematic diagram of OT1 engineered iT cells for anti-tumor therapy. Mouse MEF cells were isolated from CD45.2 + C57BL/6 mouse and reprogrammed into iPSC with Oct4, Klf4, and Sox2 retro-viruses. Then a rtTA-TRE-Runx1-Hoxa9-HygroR DNA cassette was inserted into the Rosa26 locus . Next, a CAG-OT1-IRES-GFP-PuroR expression element was inserted into the Hipp11 locus of iR9 -iPSC. OT1- iR9 -iPSC results in the production of CD8 + T cells carrying TCRVα2 and TCRVβ5 (MHC class I-restricted, ovalbumin-specific TCR). OT1- iR9 -iPSC-derived iHEC were induced into iHPC (OT1-iHPC) as described in material and method sections. The iHPC were injected into irradiated (4.5 Gy) Rag1 −/− recipient mice (3 million/mouse, 8-10-week-old C57BL/6 background). E.G7-OVA tumor cell line (C57BL/6 background) were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− (n = 8) by subcutaneous injection (0.2 million/mouse) six weeks after OT1-iHPC transplantation. b TCRVα2 and TCRVβ5 expression in OT1- iR9 -iPSC measured by intracellular staining. The iR9 -iPSC was used as negative control. c Sorting gates of the OT1 - iR9 -iPSC-derived iHEC population at day 11. The cells were enriched by streptavidin-beads recognizing <t>biotin-CD31</t> before sorting. Representative plots from three independent experiments are shown. d Immuno-phenotypes of pre-thymic progenitors in induced hematopoietic progenitor cells from OT1 - iR9 -iPSC-derived iHEC after ten-day maturation. Representative plots from three independent experiments are shown. Lin was defined as CD2 − CD3 − CD4 − CD8 − CD11b − Gr1 − Ter119 − CD19 − NK1.1 − TCRγδ − . pre-thymic progenitors were defined as Lin − c-kit + CD127 + /CD135 + . e TCRVα2 and TCRVβ5 expression of iT cells in PB of Rag1 −/− mice 6 weeks after transplantation of OT1 - iR9 -iPSC-derived iHPC. Three representative mice from three independent experiments were analyzed. f Tumor growth in Rag1 −/− and OT1-iT- Rag1 −/− mice. E.G7-OVA cells were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− mice (n = 8) by subcutaneous injection (0.2 million/mouse). The length and width of the tumors were measured every other day by a caliper, and each tumor size was calculated as length × width (mm 2 ). Mice with tumor size larger than 20 mm at the longest axis were euthanized for ethical consideration. *** P
    Cd31 Pecam 1 Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd31 pecam 1 monoclonal antibody/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd31 pecam 1 monoclonal antibody - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    86
    Takeda dexlansoprazole mr
    OT1-iT cell therapy suppresses the solid tumor growth in mice transplanted with E.G7-OVA cells a Schematic diagram of OT1 engineered iT cells for anti-tumor therapy. Mouse MEF cells were isolated from CD45.2 + C57BL/6 mouse and reprogrammed into iPSC with Oct4, Klf4, and Sox2 retro-viruses. Then a rtTA-TRE-Runx1-Hoxa9-HygroR DNA cassette was inserted into the Rosa26 locus . Next, a CAG-OT1-IRES-GFP-PuroR expression element was inserted into the Hipp11 locus of iR9 -iPSC. OT1- iR9 -iPSC results in the production of CD8 + T cells carrying TCRVα2 and TCRVβ5 (MHC class I-restricted, ovalbumin-specific TCR). OT1- iR9 -iPSC-derived iHEC were induced into iHPC (OT1-iHPC) as described in material and method sections. The iHPC were injected into irradiated (4.5 Gy) Rag1 −/− recipient mice (3 million/mouse, 8-10-week-old C57BL/6 background). E.G7-OVA tumor cell line (C57BL/6 background) were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− (n = 8) by subcutaneous injection (0.2 million/mouse) six weeks after OT1-iHPC transplantation. b TCRVα2 and TCRVβ5 expression in OT1- iR9 -iPSC measured by intracellular staining. The iR9 -iPSC was used as negative control. c Sorting gates of the OT1 - iR9 -iPSC-derived iHEC population at day 11. The cells were enriched by streptavidin-beads recognizing <t>biotin-CD31</t> before sorting. Representative plots from three independent experiments are shown. d Immuno-phenotypes of pre-thymic progenitors in induced hematopoietic progenitor cells from OT1 - iR9 -iPSC-derived iHEC after ten-day maturation. Representative plots from three independent experiments are shown. Lin was defined as CD2 − CD3 − CD4 − CD8 − CD11b − Gr1 − Ter119 − CD19 − NK1.1 − TCRγδ − . pre-thymic progenitors were defined as Lin − c-kit + CD127 + /CD135 + . e TCRVα2 and TCRVβ5 expression of iT cells in PB of Rag1 −/− mice 6 weeks after transplantation of OT1 - iR9 -iPSC-derived iHPC. Three representative mice from three independent experiments were analyzed. f Tumor growth in Rag1 −/− and OT1-iT- Rag1 −/− mice. E.G7-OVA cells were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− mice (n = 8) by subcutaneous injection (0.2 million/mouse). The length and width of the tumors were measured every other day by a caliper, and each tumor size was calculated as length × width (mm 2 ). Mice with tumor size larger than 20 mm at the longest axis were euthanized for ethical consideration. *** P
    Dexlansoprazole Mr, supplied by Takeda, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dexlansoprazole mr/product/Takeda
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dexlansoprazole mr - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    95
    BioLegend cd31
    JUP overexpression drives increased angiogenesis in B16 murine melanomas. (A) VEGF-A concentration per milligram of tumor tissue was quantitated using multiplex assay in day 14 B16-AAD control, JUP +, and PKP3 + i.p. tumors. (B) Percentage of <t>CD31</t> + pixel area was quantitated in B16-AAD control (n = 5 tumors) and JUP + (n = 4 tumors) i.p. tumors harvested at day 14 using ImageJ software. Representative immunofluorescence images stained with antibodies to CD31 (white), CD45 (green), and DAPI and analyzed of frozen sections of day 14 i.p. B16-AAD Control (C) and JUP + (D) tumors. Scale bar, 200 μm. Differences were assessed by 1-way ANOVA and unpaired t tests, with significance noted by asterisks: *
    Cd31, supplied by BioLegend, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd31/product/BioLegend
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd31 - by Bioz Stars, 2022-08
    95/100 stars
      Buy from Supplier

    Image Search Results


    OT1-iT cell therapy suppresses the solid tumor growth in mice transplanted with E.G7-OVA cells a Schematic diagram of OT1 engineered iT cells for anti-tumor therapy. Mouse MEF cells were isolated from CD45.2 + C57BL/6 mouse and reprogrammed into iPSC with Oct4, Klf4, and Sox2 retro-viruses. Then a rtTA-TRE-Runx1-Hoxa9-HygroR DNA cassette was inserted into the Rosa26 locus . Next, a CAG-OT1-IRES-GFP-PuroR expression element was inserted into the Hipp11 locus of iR9 -iPSC. OT1- iR9 -iPSC results in the production of CD8 + T cells carrying TCRVα2 and TCRVβ5 (MHC class I-restricted, ovalbumin-specific TCR). OT1- iR9 -iPSC-derived iHEC were induced into iHPC (OT1-iHPC) as described in material and method sections. The iHPC were injected into irradiated (4.5 Gy) Rag1 −/− recipient mice (3 million/mouse, 8-10-week-old C57BL/6 background). E.G7-OVA tumor cell line (C57BL/6 background) were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− (n = 8) by subcutaneous injection (0.2 million/mouse) six weeks after OT1-iHPC transplantation. b TCRVα2 and TCRVβ5 expression in OT1- iR9 -iPSC measured by intracellular staining. The iR9 -iPSC was used as negative control. c Sorting gates of the OT1 - iR9 -iPSC-derived iHEC population at day 11. The cells were enriched by streptavidin-beads recognizing biotin-CD31 before sorting. Representative plots from three independent experiments are shown. d Immuno-phenotypes of pre-thymic progenitors in induced hematopoietic progenitor cells from OT1 - iR9 -iPSC-derived iHEC after ten-day maturation. Representative plots from three independent experiments are shown. Lin was defined as CD2 − CD3 − CD4 − CD8 − CD11b − Gr1 − Ter119 − CD19 − NK1.1 − TCRγδ − . pre-thymic progenitors were defined as Lin − c-kit + CD127 + /CD135 + . e TCRVα2 and TCRVβ5 expression of iT cells in PB of Rag1 −/− mice 6 weeks after transplantation of OT1 - iR9 -iPSC-derived iHPC. Three representative mice from three independent experiments were analyzed. f Tumor growth in Rag1 −/− and OT1-iT- Rag1 −/− mice. E.G7-OVA cells were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− mice (n = 8) by subcutaneous injection (0.2 million/mouse). The length and width of the tumors were measured every other day by a caliper, and each tumor size was calculated as length × width (mm 2 ). Mice with tumor size larger than 20 mm at the longest axis were euthanized for ethical consideration. *** P

    Journal: bioRxiv

    Article Title: Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors

    doi: 10.1101/660977

    Figure Lengend Snippet: OT1-iT cell therapy suppresses the solid tumor growth in mice transplanted with E.G7-OVA cells a Schematic diagram of OT1 engineered iT cells for anti-tumor therapy. Mouse MEF cells were isolated from CD45.2 + C57BL/6 mouse and reprogrammed into iPSC with Oct4, Klf4, and Sox2 retro-viruses. Then a rtTA-TRE-Runx1-Hoxa9-HygroR DNA cassette was inserted into the Rosa26 locus . Next, a CAG-OT1-IRES-GFP-PuroR expression element was inserted into the Hipp11 locus of iR9 -iPSC. OT1- iR9 -iPSC results in the production of CD8 + T cells carrying TCRVα2 and TCRVβ5 (MHC class I-restricted, ovalbumin-specific TCR). OT1- iR9 -iPSC-derived iHEC were induced into iHPC (OT1-iHPC) as described in material and method sections. The iHPC were injected into irradiated (4.5 Gy) Rag1 −/− recipient mice (3 million/mouse, 8-10-week-old C57BL/6 background). E.G7-OVA tumor cell line (C57BL/6 background) were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− (n = 8) by subcutaneous injection (0.2 million/mouse) six weeks after OT1-iHPC transplantation. b TCRVα2 and TCRVβ5 expression in OT1- iR9 -iPSC measured by intracellular staining. The iR9 -iPSC was used as negative control. c Sorting gates of the OT1 - iR9 -iPSC-derived iHEC population at day 11. The cells were enriched by streptavidin-beads recognizing biotin-CD31 before sorting. Representative plots from three independent experiments are shown. d Immuno-phenotypes of pre-thymic progenitors in induced hematopoietic progenitor cells from OT1 - iR9 -iPSC-derived iHEC after ten-day maturation. Representative plots from three independent experiments are shown. Lin was defined as CD2 − CD3 − CD4 − CD8 − CD11b − Gr1 − Ter119 − CD19 − NK1.1 − TCRγδ − . pre-thymic progenitors were defined as Lin − c-kit + CD127 + /CD135 + . e TCRVα2 and TCRVβ5 expression of iT cells in PB of Rag1 −/− mice 6 weeks after transplantation of OT1 - iR9 -iPSC-derived iHPC. Three representative mice from three independent experiments were analyzed. f Tumor growth in Rag1 −/− and OT1-iT- Rag1 −/− mice. E.G7-OVA cells were transplanted into the groin of the Rag1 −/− (n = 8) or OT1-iT- Rag1 −/− mice (n = 8) by subcutaneous injection (0.2 million/mouse). The length and width of the tumors were measured every other day by a caliper, and each tumor size was calculated as length × width (mm 2 ). Mice with tumor size larger than 20 mm at the longest axis were euthanized for ethical consideration. *** P

    Article Snippet: The following antibodies were used: c-kit (2B8, eBioscience), CD31 (390, eBioscience), CD41 (eBioMWReg30, eBioscience), CD45 (30-F11, eBioscience), CD45.1 (A20, eBioscience), CD45.2 (104, eBioscience), CD2 (RM2-5, eBioscience), CD3 (145-2C11, eBioscience), CD4 (GK1.5, eBioscience), CD8a (53-6.7, eBioscience), CD19(eBio1D3, eBioscience), B220 (RA3-6B2, eBioscience), CD11b (M1/70, eBioscience), NK1.1 (PK136, eBioscience), Ter119 (TER-119, eBioscience), Gr1 (RB6-8C5, eBioscience), CD201 (eBio1560, eBioscience), CD135 (A2F10, eBioscience), CD127 (A7R34 eBioscience) FcεRIα (MAR-1, biolegend), CD69 (H1.2F3, biolegend), CD62L (MEL-14, biolegend) IFNγ (XMG1.2, biolegend), IL17 (TC11-18H10.1, biolegend), CD44 (IM7, eBioscience), CD25 (PC61.5, eBioscience), TCRβ (H57-597, eBioscience), TCRγδ (GL3, eBioscience), TCRvα2 (B20.1, biolegend), TCRvβ5.1/5.2 (MR9-4, biolegend) Streptavidin PE-Cy7 (eBioscience), Streptavidin eFlour 450 (eBioscience), Streptavidin PE-Cy5 (biolegend).

    Techniques: Mouse Assay, Isolation, Expressing, Derivative Assay, Injection, Irradiation, Transplantation Assay, Staining, Negative Control

    Single-cell transcriptomic characterization of iHEC and iHPC a Principal component analysis (PCA) of iHEC and developmental E11 AGM-derived EC, T1 pre-HSC, T2 pre-HSC, E12 HSC, E14 HSC, and adult HSC. The TPM values of iHEC (n = 70), natural E11 AGM-derived EC (n = 17), T1 pre-HSC (n = 28), T2 pre-HSC (n = 32), E12 HSC (n = 21), E14 HSC (n = 32) and adult HSC (n = 47) single-cell RNA-Seq data were calculated with Stringtie package. b The expression of the top 100 genes contributing most to PC2 (50 genes for each direction). The expression value (TPM) of each gene was converted by log2 and illustrated by pheatmap (R package). One column represents one cell repeat. c Violin plots show the expression profile of selected artery (A) and vein (V) related genes (A: Nrp1 , Efnb2 , and Hey1 ; V: Nrp2 , Nr2f2 , and Ephb4 ) in single iHEC. The expression value (TPM) of each gene was converted by log2 and illustrated by ggplot2 (R package). One point represents one cell. d Violin plots show the expression profile of selected surface markers ( Cdh5 , Esam , Tek , Procr , Cd47 , and Cd63 ) in single iHEC. The expression value (TPM) of each gene was converted by log2 and illustrated by ggplot2 (R package). One point represents one cell. e Violin plots show the expression profile of selected transcription factors ( Fli1 , Erg1 , Lmo2 , Lyl1 , Tal1 , Sox7 , Runx1 , Mycn , Gata2 , Bcl11a , Hoxa9 , and Hoxb5 ) related to hematopoietic development in single iHEC. The expression value (TPM) of each gene was converted by log2 and illustrated by ggplot2 (R package). One point represents one cell. f Two-dimensional tSNE analysis of iHEC and iHPC single-cell RNA-Seq. For single-cell RNA-Seq, the iHEC were collected on day 11, and the iHPC were collected at Day14, 17 and 21. Each dot represents one cell. The TPM values of iHEC (n = 65), iHPC at Day14 (n = 21), Day17 (n = 18) and Day21 (n = 56) from single-cell RNA-Seq data were calculated with Stringtie package. Cell types were defined as: iHEC CD31 + CD41 low CD45 − c-kit + CD201 high ; Day14 and Day17 iHPC, CD45 + Lin (Ter119/Gr1/F4-80/CD2/CD3/CD4/CD8/CD19/FcεRIα) − ; Day21 iHPC Ter119 − CD45 + c-kit + CD127 + . g tSNE analysis of the expression pattern of selected endothelia-related transcription factors ( Sox7 , Sox18 , and Ets1 ) in iHEC and iHPC. h tSNE analysis of the expression pattern of selected hematopoietic-related transcription factors ( Lyl1 , Etv6 , Prdm5 , Myb , Sfpi1 , and Meis1 ) in iHEC and iHPC. i tSNE analysis of the expression pattern of selected T cell development-related transcription factors ( Lmo2 , Bcl11a , Ikzf1 , Myc , Gata3 , and Tcf7 ) in iHEC and iHPC at Day14, Day17, and Day21. j tSNE analysis of the expression pattern of selected lymphopoiesis-related surface protein-coding genes ( Kit , Flt3 , Cd7 , Ccr9 , Ccr7 , and Cxcr4 ) in iHEC and iHPC at Day14, Day17, and Day21.

    Journal: bioRxiv

    Article Title: Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors

    doi: 10.1101/660977

    Figure Lengend Snippet: Single-cell transcriptomic characterization of iHEC and iHPC a Principal component analysis (PCA) of iHEC and developmental E11 AGM-derived EC, T1 pre-HSC, T2 pre-HSC, E12 HSC, E14 HSC, and adult HSC. The TPM values of iHEC (n = 70), natural E11 AGM-derived EC (n = 17), T1 pre-HSC (n = 28), T2 pre-HSC (n = 32), E12 HSC (n = 21), E14 HSC (n = 32) and adult HSC (n = 47) single-cell RNA-Seq data were calculated with Stringtie package. b The expression of the top 100 genes contributing most to PC2 (50 genes for each direction). The expression value (TPM) of each gene was converted by log2 and illustrated by pheatmap (R package). One column represents one cell repeat. c Violin plots show the expression profile of selected artery (A) and vein (V) related genes (A: Nrp1 , Efnb2 , and Hey1 ; V: Nrp2 , Nr2f2 , and Ephb4 ) in single iHEC. The expression value (TPM) of each gene was converted by log2 and illustrated by ggplot2 (R package). One point represents one cell. d Violin plots show the expression profile of selected surface markers ( Cdh5 , Esam , Tek , Procr , Cd47 , and Cd63 ) in single iHEC. The expression value (TPM) of each gene was converted by log2 and illustrated by ggplot2 (R package). One point represents one cell. e Violin plots show the expression profile of selected transcription factors ( Fli1 , Erg1 , Lmo2 , Lyl1 , Tal1 , Sox7 , Runx1 , Mycn , Gata2 , Bcl11a , Hoxa9 , and Hoxb5 ) related to hematopoietic development in single iHEC. The expression value (TPM) of each gene was converted by log2 and illustrated by ggplot2 (R package). One point represents one cell. f Two-dimensional tSNE analysis of iHEC and iHPC single-cell RNA-Seq. For single-cell RNA-Seq, the iHEC were collected on day 11, and the iHPC were collected at Day14, 17 and 21. Each dot represents one cell. The TPM values of iHEC (n = 65), iHPC at Day14 (n = 21), Day17 (n = 18) and Day21 (n = 56) from single-cell RNA-Seq data were calculated with Stringtie package. Cell types were defined as: iHEC CD31 + CD41 low CD45 − c-kit + CD201 high ; Day14 and Day17 iHPC, CD45 + Lin (Ter119/Gr1/F4-80/CD2/CD3/CD4/CD8/CD19/FcεRIα) − ; Day21 iHPC Ter119 − CD45 + c-kit + CD127 + . g tSNE analysis of the expression pattern of selected endothelia-related transcription factors ( Sox7 , Sox18 , and Ets1 ) in iHEC and iHPC. h tSNE analysis of the expression pattern of selected hematopoietic-related transcription factors ( Lyl1 , Etv6 , Prdm5 , Myb , Sfpi1 , and Meis1 ) in iHEC and iHPC. i tSNE analysis of the expression pattern of selected T cell development-related transcription factors ( Lmo2 , Bcl11a , Ikzf1 , Myc , Gata3 , and Tcf7 ) in iHEC and iHPC at Day14, Day17, and Day21. j tSNE analysis of the expression pattern of selected lymphopoiesis-related surface protein-coding genes ( Kit , Flt3 , Cd7 , Ccr9 , Ccr7 , and Cxcr4 ) in iHEC and iHPC at Day14, Day17, and Day21.

    Article Snippet: The following antibodies were used: c-kit (2B8, eBioscience), CD31 (390, eBioscience), CD41 (eBioMWReg30, eBioscience), CD45 (30-F11, eBioscience), CD45.1 (A20, eBioscience), CD45.2 (104, eBioscience), CD2 (RM2-5, eBioscience), CD3 (145-2C11, eBioscience), CD4 (GK1.5, eBioscience), CD8a (53-6.7, eBioscience), CD19(eBio1D3, eBioscience), B220 (RA3-6B2, eBioscience), CD11b (M1/70, eBioscience), NK1.1 (PK136, eBioscience), Ter119 (TER-119, eBioscience), Gr1 (RB6-8C5, eBioscience), CD201 (eBio1560, eBioscience), CD135 (A2F10, eBioscience), CD127 (A7R34 eBioscience) FcεRIα (MAR-1, biolegend), CD69 (H1.2F3, biolegend), CD62L (MEL-14, biolegend) IFNγ (XMG1.2, biolegend), IL17 (TC11-18H10.1, biolegend), CD44 (IM7, eBioscience), CD25 (PC61.5, eBioscience), TCRβ (H57-597, eBioscience), TCRγδ (GL3, eBioscience), TCRvα2 (B20.1, biolegend), TCRvβ5.1/5.2 (MR9-4, biolegend) Streptavidin PE-Cy7 (eBioscience), Streptavidin eFlour 450 (eBioscience), Streptavidin PE-Cy5 (biolegend).

    Techniques: Derivative Assay, RNA Sequencing Assay, Expressing

    JUP overexpression drives increased angiogenesis in B16 murine melanomas. (A) VEGF-A concentration per milligram of tumor tissue was quantitated using multiplex assay in day 14 B16-AAD control, JUP +, and PKP3 + i.p. tumors. (B) Percentage of CD31 + pixel area was quantitated in B16-AAD control (n = 5 tumors) and JUP + (n = 4 tumors) i.p. tumors harvested at day 14 using ImageJ software. Representative immunofluorescence images stained with antibodies to CD31 (white), CD45 (green), and DAPI and analyzed of frozen sections of day 14 i.p. B16-AAD Control (C) and JUP + (D) tumors. Scale bar, 200 μm. Differences were assessed by 1-way ANOVA and unpaired t tests, with significance noted by asterisks: *

    Journal: Annals of surgery

    Article Title: The Barrier Molecules Junction Plakoglobin, Filaggrin, and Dystonin Play Roles in Melanoma Growth and Angiogenesis

    doi: 10.1097/SLA.0000000000003522

    Figure Lengend Snippet: JUP overexpression drives increased angiogenesis in B16 murine melanomas. (A) VEGF-A concentration per milligram of tumor tissue was quantitated using multiplex assay in day 14 B16-AAD control, JUP +, and PKP3 + i.p. tumors. (B) Percentage of CD31 + pixel area was quantitated in B16-AAD control (n = 5 tumors) and JUP + (n = 4 tumors) i.p. tumors harvested at day 14 using ImageJ software. Representative immunofluorescence images stained with antibodies to CD31 (white), CD45 (green), and DAPI and analyzed of frozen sections of day 14 i.p. B16-AAD Control (C) and JUP + (D) tumors. Scale bar, 200 μm. Differences were assessed by 1-way ANOVA and unpaired t tests, with significance noted by asterisks: *

    Article Snippet: Fluorophore- or biotin-conjugated antibodies specific for murine cell surface antigens and intracellular proteins are as follows: CD45 (30-F11), CD3 (145–2C11), CD4 (GK1.5), CD8 (53–6.7), CD44 (IM7), IFNγ (XMG1.2), FoxP3 (FJK-16 s), and CD31 (390) were from Biolegend.

    Techniques: Over Expression, Concentration Assay, Multiplex Assay, Software, Immunofluorescence, Staining