Article Title: Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals
Figure Lengend Snippet: Rare immature Sertoli cells in XY and XX Rspo1 KO Sox8 KO Sox9 cKO triple mutant gonads. Both XY or XX triple mutants exhibited rare cells expressing DMRT1, a Sertoli cell marker, and GATA4, a somatic cell marker (asterisks in C’ and C’’, and in I’ and I’’), but lacked DMRT1-postive cells Sertoli cells expressing the mature Sertoli cell marker GATA1 (F’, L’, O’). Cells expressing DMRT1 (F, L, O) were sometimes found in close proximity with cells expressing the granulosa cell marker FOXL2 (F’’, F’’’, L’’, L’’’, O’’, O’’’), and some cells co-expressed DMRT1 and FOXL2 (L”, L”’, arrowheads). Though GATA1-postive cells are absent in XX Rspo1 KO Sox9 cKO mice at P10 (E), they are present in P40 gonads (K, N). Scale bars 50μm.
Article Snippet: The following dilutions of primary antibodies were used: AMH/MIS (c-20, sc-6886, Santa Cruz), 1:200; DMRT1 (HPA027850, Sigma), 1:100; FOXL2 (NB100-1277, Novus), 1:200; GATA1 (N6, sc-265, Santa Cruz), 1:200; GATA4 (C20, sc-1237, Santa Cruz), 1:200; 3βHSD (P18, sc-30820, Santa Cruz), 1:200; P27 (Kip1, sc-528, Santa Cruz), 1:200; SF1 (kindly provided by Ken Morohashi), 1:1000; SOX9 (HPA001758, Sigma), 1:200; and TRA98 (ab82527, Abcam), 1:200.
Techniques: Mutagenesis, Expressing, Marker, Mouse Assay