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  • 86
    Novartis raf265
    Raf265, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raf265/product/Novartis
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    raf265 - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology gata 1
    Rare immature Sertoli cells in XY and XX Rspo1 KO Sox8 KO Sox9 cKO triple mutant gonads. Both XY or XX triple mutants exhibited rare cells expressing DMRT1, a Sertoli cell marker, and GATA4, a somatic cell marker (asterisks in C’ and C’’, and in I’ and I’’), but lacked DMRT1-postive cells Sertoli cells expressing the mature Sertoli cell marker <t>GATA1</t> (F’, L’, O’). Cells expressing DMRT1 (F, L, O) were sometimes found in close proximity with cells expressing the granulosa cell marker FOXL2 (F’’, F’’’, L’’, L’’’, O’’, O’’’), and some cells co-expressed DMRT1 and FOXL2 (L”, L”’, arrowheads). Though GATA1-postive cells are absent in XX Rspo1 KO Sox9 cKO mice at P10 (E), they are present in P40 gonads (K, N). Scale bars 50μm.
    Gata 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gata 1/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gata 1 - by Bioz Stars, 2022-07
    96/100 stars
      Buy from Supplier

    Image Search Results


    Rare immature Sertoli cells in XY and XX Rspo1 KO Sox8 KO Sox9 cKO triple mutant gonads. Both XY or XX triple mutants exhibited rare cells expressing DMRT1, a Sertoli cell marker, and GATA4, a somatic cell marker (asterisks in C’ and C’’, and in I’ and I’’), but lacked DMRT1-postive cells Sertoli cells expressing the mature Sertoli cell marker GATA1 (F’, L’, O’). Cells expressing DMRT1 (F, L, O) were sometimes found in close proximity with cells expressing the granulosa cell marker FOXL2 (F’’, F’’’, L’’, L’’’, O’’, O’’’), and some cells co-expressed DMRT1 and FOXL2 (L”, L”’, arrowheads). Though GATA1-postive cells are absent in XX Rspo1 KO Sox9 cKO mice at P10 (E), they are present in P40 gonads (K, N). Scale bars 50μm.

    Journal: bioRxiv

    Article Title: Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals

    doi: 10.1101/2019.12.13.875443

    Figure Lengend Snippet: Rare immature Sertoli cells in XY and XX Rspo1 KO Sox8 KO Sox9 cKO triple mutant gonads. Both XY or XX triple mutants exhibited rare cells expressing DMRT1, a Sertoli cell marker, and GATA4, a somatic cell marker (asterisks in C’ and C’’, and in I’ and I’’), but lacked DMRT1-postive cells Sertoli cells expressing the mature Sertoli cell marker GATA1 (F’, L’, O’). Cells expressing DMRT1 (F, L, O) were sometimes found in close proximity with cells expressing the granulosa cell marker FOXL2 (F’’, F’’’, L’’, L’’’, O’’, O’’’), and some cells co-expressed DMRT1 and FOXL2 (L”, L”’, arrowheads). Though GATA1-postive cells are absent in XX Rspo1 KO Sox9 cKO mice at P10 (E), they are present in P40 gonads (K, N). Scale bars 50μm.

    Article Snippet: The following dilutions of primary antibodies were used: AMH/MIS (c-20, sc-6886, Santa Cruz), 1:200; DMRT1 (HPA027850, Sigma), 1:100; FOXL2 (NB100-1277, Novus), 1:200; GATA1 (N6, sc-265, Santa Cruz), 1:200; GATA4 (C20, sc-1237, Santa Cruz), 1:200; 3βHSD (P18, sc-30820, Santa Cruz), 1:200; P27 (Kip1, sc-528, Santa Cruz), 1:200; SF1 (kindly provided by Ken Morohashi), 1:1000; SOX9 (HPA001758, Sigma), 1:200; and TRA98 (ab82527, Abcam), 1:200.

    Techniques: Mutagenesis, Expressing, Marker, Mouse Assay

    Validation of DNA binding protein occupancy. ChIP analysis of K562 chromatin for occupancy by the erythroid-specific transcription factors GATA1 and NF-E2; RNA Pol II; the chromatin architectural protein CTCF; and the barrier-associated proteins USF1,

    Journal: Blood

    Article Title: A tissue-specific chromatin loop activates the erythroid ankyrin-1 promoter

    doi: 10.1182/blood-2012-08-450262

    Figure Lengend Snippet: Validation of DNA binding protein occupancy. ChIP analysis of K562 chromatin for occupancy by the erythroid-specific transcription factors GATA1 and NF-E2; RNA Pol II; the chromatin architectural protein CTCF; and the barrier-associated proteins USF1,

    Article Snippet: ChIP was performed using the Magna ChIP A assay kit (17-610; Millipore), with anti-GATA1 (sc-265), –NF-E2 (sc-22 827), -USF2 (sc-862), -USF1 (sc-8983), -PRMT1, and -RNA polymerase II (sc-130851; all from Santa Cruz Biotechnology), -CTCF (07-729; Millipore), or -CARM1 (PRMT4, ab84370; Abcam) antibodies.

    Techniques: Binding Assay, Chromatin Immunoprecipitation