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  • gli3  (Abcam)
    95
    Abcam gli3
    Expression of Hh signalling pathway effectors in embryos. At E12.5, compared to vehicle-exposed embryos, immunofluorescent staining for Ptch1 ( a , b ) and Gli1 ( e , f ) in coronal sections of GD10.5 embryos showed a slight reduction in the frontal bone primordium (dotted white lines), and an even greater decrease was observed in <t>Gli3</t> expression ( i , j ) in the frontal bone primordium. At E13.5, Ptch1 ( c , d ), Gli1 ( g , h ) and Gli3 ( k , l ) expression levels were markedly diminished in the frontal bone primordium of GD10.5 embryos compared with vehicle-exposed embryos. Consistent with this finding, the sections in the white boxed areas show lower magnification views of the frontal bone primordium. Arrowheads point to Gli3-negative cells. m There was a significant reduction in p = Ptch1, Gli1 and Gli3 intensity in the frontal bone primordium in GD10.5 embryos in comparison to vehicle-exposed embryos ( n = 6 embryos per group from three litters). The values are the mean ± SD. Data were analysed using unpaired Student’s t tests. ** P
    Gli3, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gli3/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gli3 - by Bioz Stars, 2022-01
    95/100 stars
      Buy from Supplier

    95
    Becton Dickinson ras
    Expression of Hh signalling pathway effectors in embryos. At E12.5, compared to vehicle-exposed embryos, immunofluorescent staining for Ptch1 ( a , b ) and Gli1 ( e , f ) in coronal sections of GD10.5 embryos showed a slight reduction in the frontal bone primordium (dotted white lines), and an even greater decrease was observed in <t>Gli3</t> expression ( i , j ) in the frontal bone primordium. At E13.5, Ptch1 ( c , d ), Gli1 ( g , h ) and Gli3 ( k , l ) expression levels were markedly diminished in the frontal bone primordium of GD10.5 embryos compared with vehicle-exposed embryos. Consistent with this finding, the sections in the white boxed areas show lower magnification views of the frontal bone primordium. Arrowheads point to Gli3-negative cells. m There was a significant reduction in p = Ptch1, Gli1 and Gli3 intensity in the frontal bone primordium in GD10.5 embryos in comparison to vehicle-exposed embryos ( n = 6 embryos per group from three litters). The values are the mean ± SD. Data were analysed using unpaired Student’s t tests. ** P
    Ras, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ras/product/Becton Dickinson
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ras - by Bioz Stars, 2022-01
    95/100 stars
      Buy from Supplier

    93
    Thermo Fisher claudin 2
    Expression of Hh signalling pathway effectors in embryos. At E12.5, compared to vehicle-exposed embryos, immunofluorescent staining for Ptch1 ( a , b ) and Gli1 ( e , f ) in coronal sections of GD10.5 embryos showed a slight reduction in the frontal bone primordium (dotted white lines), and an even greater decrease was observed in <t>Gli3</t> expression ( i , j ) in the frontal bone primordium. At E13.5, Ptch1 ( c , d ), Gli1 ( g , h ) and Gli3 ( k , l ) expression levels were markedly diminished in the frontal bone primordium of GD10.5 embryos compared with vehicle-exposed embryos. Consistent with this finding, the sections in the white boxed areas show lower magnification views of the frontal bone primordium. Arrowheads point to Gli3-negative cells. m There was a significant reduction in p = Ptch1, Gli1 and Gli3 intensity in the frontal bone primordium in GD10.5 embryos in comparison to vehicle-exposed embryos ( n = 6 embryos per group from three litters). The values are the mean ± SD. Data were analysed using unpaired Student’s t tests. ** P
    Claudin 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/claudin 2/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    claudin 2 - by Bioz Stars, 2022-01
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expression of Hh signalling pathway effectors in embryos. At E12.5, compared to vehicle-exposed embryos, immunofluorescent staining for Ptch1 ( a , b ) and Gli1 ( e , f ) in coronal sections of GD10.5 embryos showed a slight reduction in the frontal bone primordium (dotted white lines), and an even greater decrease was observed in Gli3 expression ( i , j ) in the frontal bone primordium. At E13.5, Ptch1 ( c , d ), Gli1 ( g , h ) and Gli3 ( k , l ) expression levels were markedly diminished in the frontal bone primordium of GD10.5 embryos compared with vehicle-exposed embryos. Consistent with this finding, the sections in the white boxed areas show lower magnification views of the frontal bone primordium. Arrowheads point to Gli3-negative cells. m There was a significant reduction in p = Ptch1, Gli1 and Gli3 intensity in the frontal bone primordium in GD10.5 embryos in comparison to vehicle-exposed embryos ( n = 6 embryos per group from three litters). The values are the mean ± SD. Data were analysed using unpaired Student’s t tests. ** P

    Journal: International Journal of Oral Science

    Article Title: Defining a critical period in calvarial development for Hedgehog pathway antagonist-induced frontal bone dysplasia in mice

    doi: 10.1038/s41368-018-0040-z

    Figure Lengend Snippet: Expression of Hh signalling pathway effectors in embryos. At E12.5, compared to vehicle-exposed embryos, immunofluorescent staining for Ptch1 ( a , b ) and Gli1 ( e , f ) in coronal sections of GD10.5 embryos showed a slight reduction in the frontal bone primordium (dotted white lines), and an even greater decrease was observed in Gli3 expression ( i , j ) in the frontal bone primordium. At E13.5, Ptch1 ( c , d ), Gli1 ( g , h ) and Gli3 ( k , l ) expression levels were markedly diminished in the frontal bone primordium of GD10.5 embryos compared with vehicle-exposed embryos. Consistent with this finding, the sections in the white boxed areas show lower magnification views of the frontal bone primordium. Arrowheads point to Gli3-negative cells. m There was a significant reduction in p = Ptch1, Gli1 and Gli3 intensity in the frontal bone primordium in GD10.5 embryos in comparison to vehicle-exposed embryos ( n = 6 embryos per group from three litters). The values are the mean ± SD. Data were analysed using unpaired Student’s t tests. ** P

    Article Snippet: For immunofluorescence, antibodies were obtained and diluted as follows: anti-Ptch1 (1:200, Abcam), anti-Gli1 (1:400, Abcam) and Gli3 (1:300, Abcam).

    Techniques: Expressing, Staining