Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring

doi: 10.3389/fcimb.2022.799501

Figure Lengend Snippet: Demographic differences between participants based on high versus expected in vivo TFV release rates.

Article Snippet: Our PD modeling data also support that the TFV VF concentrations achieved provided significant increases in median HIV-1 inhibition, for women with either LbD CSTs (96% and 97%, at months 1 and 3, respectively) or CST IVA/B (99% and 74% at months 1 and 3, respectively) vaginal microbiota over the median baseline (14% and 4% for LbD and CSTIV, respectively), pre-insertion HIV-1 inhibition levels.

Techniques: In Vivo, Standard Deviation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring

doi: 10.3389/fcimb.2022.799501

Figure Lengend Snippet: Vaginal fluid TFV concentrations (ng/mg) and vaginal tissue TFV (ng/mg) and TFV-DP (fmol/mg) concentrations among TFV/LNG IVR users (cyclic and continuous cohorts combined) with either Lactobacillus dominated (LbD) or CST IVA/B microbiota.

Article Snippet: Our PD modeling data also support that the TFV VF concentrations achieved provided significant increases in median HIV-1 inhibition, for women with either LbD CSTs (96% and 97%, at months 1 and 3, respectively) or CST IVA/B (99% and 74% at months 1 and 3, respectively) vaginal microbiota over the median baseline (14% and 4% for LbD and CSTIV, respectively), pre-insertion HIV-1 inhibition levels.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring

doi: 10.3389/fcimb.2022.799501

Figure Lengend Snippet: (A–C) Correlation of tenofovir (TFV) vaginal fluid concentration with the relative abundance of Gardnerella vaginalis at months 1, 2, and 3. Green dots represent participants who had Lactobacillus-dominated (LbD) vaginal microbiota at the time of sampling and their individual TFV vaginal fluid concentration and relative abundance of Gardnerella vaginalis . Blue dots represent participants who had diverse anaerobe-dominated (CST IVA/B) vaginal microbiota at the time of sampling and their individual TFV vaginal fluid concentration and relative abundance of Gardnerella vaginalis .

Article Snippet: Our PD modeling data also support that the TFV VF concentrations achieved provided significant increases in median HIV-1 inhibition, for women with either LbD CSTs (96% and 97%, at months 1 and 3, respectively) or CST IVA/B (99% and 74% at months 1 and 3, respectively) vaginal microbiota over the median baseline (14% and 4% for LbD and CSTIV, respectively), pre-insertion HIV-1 inhibition levels.

Techniques: Concentration Assay, Sampling

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring

doi: 10.3389/fcimb.2022.799501

Figure Lengend Snippet: (A–C) Correlation of tenofovir (TFV) vaginal fluid concentration with the relative abundance of Prevotella species at months 1, 2, and 3. Green dots represent participants who had Lactobacillus-dominated (LbD) vaginal microbiota at the time of sampling and their individual TFV vaginal fluid concentration and relative abundance of Prevotella species. Blue dots represent participants who had diverse anaerobe dominated (CST IVA/B) vaginal microbiota at the time of sampling and their individual TFV vaginal fluid concentration and relative abundance of Prevotella species.

Article Snippet: Our PD modeling data also support that the TFV VF concentrations achieved provided significant increases in median HIV-1 inhibition, for women with either LbD CSTs (96% and 97%, at months 1 and 3, respectively) or CST IVA/B (99% and 74% at months 1 and 3, respectively) vaginal microbiota over the median baseline (14% and 4% for LbD and CSTIV, respectively), pre-insertion HIV-1 inhibition levels.

Techniques: Concentration Assay, Sampling

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring

doi: 10.3389/fcimb.2022.799501

Figure Lengend Snippet: (A–D) Correlation of TFV (A) and TFV-DP (C) tissue concentrations with relative abundance of G. vaginalis species and fit of the linear model with CST IV samples at the end of treatment with TFV tissue concentrations (B) and TFV-DP tissue concentrations (D) . Green dots represent participants who had Lactobacillus-dominated (LbD) vaginal microbiota at the time of sampling and their individual TFV vaginal fluid concentration and relative abundance of Gardnerella vaginalis . Blue dots represent participants who had diverse anaerobe-dominated (CST IVA/B) vaginal microbiota at the time of sampling and their individual TFV vaginal fluid concentration and relative abundance of Gardnerella vaginalis . Black dots in the linear fit model represent participants who had diverse anaerobe-dominated (CST IVA/B) vaginal microbiota at the time of sampling and their individual TFV vaginal fluid concentration and relative abundance of Gardnerella vaginalis .

Article Snippet: Our PD modeling data also support that the TFV VF concentrations achieved provided significant increases in median HIV-1 inhibition, for women with either LbD CSTs (96% and 97%, at months 1 and 3, respectively) or CST IVA/B (99% and 74% at months 1 and 3, respectively) vaginal microbiota over the median baseline (14% and 4% for LbD and CSTIV, respectively), pre-insertion HIV-1 inhibition levels.

Techniques: Sampling, Concentration Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring

doi: 10.3389/fcimb.2022.799501

Figure Lengend Snippet: Percent inhibition of HIV in vitro by cervicovaginal fluid lavage (CVL) supernatant obtained from study participants using the TFV/LNG IVR at baseline (BL) pre-insertion, month 1 (M1), and end of treatment month 3 (M3). Red dots represent participant data from individuals with diverse anaerobic CST IVA/B microbiota at sampling. Blue dots represent participant data from individuals with Lactobacillus-dominated (LbD) microbiota at sampling. p values < 0.01 paired change from BL in percent HIV inhibition at months 1 and months 3 for both LbD and CST IVA/B groups.

Article Snippet: Our PD modeling data also support that the TFV VF concentrations achieved provided significant increases in median HIV-1 inhibition, for women with either LbD CSTs (96% and 97%, at months 1 and 3, respectively) or CST IVA/B (99% and 74% at months 1 and 3, respectively) vaginal microbiota over the median baseline (14% and 4% for LbD and CSTIV, respectively), pre-insertion HIV-1 inhibition levels.

Techniques: Inhibition, In Vitro, Sampling

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring

doi: 10.3389/fcimb.2022.799501

Figure Lengend Snippet: Cumulative (CUMU) p24 antigen production from cervicovaginal (CV) tissue biopsies obtained from all participants at baseline and from participants using the placebo IVR (BL). CUMU p24 antigen production from CV tissue biopsies obtained from participants using the TFV/LNG IVR at end of treatment (EOT). Red dots represent participant data from individuals with diverse anaerobic CST IVA/B microbiota at sampling. Blue dots represent participant data from individuals with Lactobacillus-dominated (LbD) microbiota at sampling. p values > 0.05 for change in p24 antigen production from tissues at EOT compared to BL.

Article Snippet: Our PD modeling data also support that the TFV VF concentrations achieved provided significant increases in median HIV-1 inhibition, for women with either LbD CSTs (96% and 97%, at months 1 and 3, respectively) or CST IVA/B (99% and 74% at months 1 and 3, respectively) vaginal microbiota over the median baseline (14% and 4% for LbD and CSTIV, respectively), pre-insertion HIV-1 inhibition levels.

Techniques: Sampling