Journal: iScience

Article Title: The indirect corticopontine pathway relays perioral sensory signals to the cerebellum via the mesodiencephalic junction

doi: 10.1016/j.isci.2023.107301

Figure Lengend Snippet:

Article Snippet: pAAV-CamKII-ArchT-GFP (PV2527) , Han et al. , Addgene 99039-AAVrg.

Techniques: Recombinant, Staining, Software, Fluorescence, Microscopy

Journal: Annals of Neurology

Article Title: Distinct Roles of Rodent Thalamus and Corpus Callosum in Seizure Generalization

doi: 10.1002/ana.26338

Figure Lengend Snippet: The activated ipsilateral thalamus sends minimal projections to the contralateral thalamus. (A) Only the right thalamus strongly expressed tdTomato. Dotted lines indicate nuclear boundaries. On the right are enlarged images of cellular tdTomato expression in the MD and VL immunolabeled for NeuN (green). (B) The right reticular thalamic nucleus (RTN) expressed more tdTomato than the left. RTN is immunolabeled for parvalbumin (PV, green). On the left are enlarged images of cellular tdTomato expression in the right and left RTN. (C) Minimal tdTomato expression is in the thalamus of steel wire implanted mice without seizures. (D) AAV9‐eGFP injection is in the right premotor cortex for E. (E) The right motor cortex projects to the right motor thalamic nuclei VM and VL. (F) AAV9 eGFP or RetroBeads injection is in the right VL for G and H. (G) The right motor cortex sends projections to the right thalamus from deep cortical layers 5 and 6 after retrograde RetroBeads injection in the right VL as indicated in F. (H) The right thalamic nuclei have minimal monosynaptic projections to the left thalamic nuclei. CM = centromedian; MD = mediodorsal; VL = ventrolateral; VM = ventromedial.

Article Snippet: We injected C57Bl/6 mice with AAV9 CamKII‐ArchT‐GFP (#99039; Addgene, 120 nl) in the right VL and allowed 2 weeks for expression.

Techniques: Expressing, Immunolabeling, Injection

Journal: Annals of Neurology

Article Title: Distinct Roles of Rodent Thalamus and Corpus Callosum in Seizure Generalization

doi: 10.1002/ana.26338

Figure Lengend Snippet: Closed‐loop optogenetic inhibition of the ipsilateral VL does not change the onset latency in the contralateral VL or contralateral cortex. (A) ArchT‐GFP expressed in the right VL. (B) AAV9 CamKII‐ArchT‐GFP was injected in the right VL. (C) Seizures that were uninterrupted (no pulse) and interrupted (with opto pulse [black bars], green background in the right VL) in the same mouse with the corresponding power spectrums. (D) Seizure onset was delayed only in the right VL after pulse activation, whereas onset latency in the left cortex or left VL did not change. LFP = local field potential; VL = ventrolateral.

Article Snippet: We injected C57Bl/6 mice with AAV9 CamKII‐ArchT‐GFP (#99039; Addgene, 120 nl) in the right VL and allowed 2 weeks for expression.

Techniques: Inhibition, Injection, Activation Assay

Journal: Annals of Neurology

Article Title: Distinct Roles of Rodent Thalamus and Corpus Callosum in Seizure Generalization

doi: 10.1002/ana.26338

Figure Lengend Snippet: The seizure focus sends extensive direct projections across the corpus callosum contralaterally. (A) The tdTomato and AAV9 GFP colocalized in the axons of the corpus callosum that connects the seizure focus with the contralateral premotor cortex. The white rectangle area is magnified on the right. (B) Top: A confocal image of Cre‐driven AAV9 GFP expression in the corpus callosum of TRAP2 mice. Middle: Super‐resolution SoRa image of the callosal axons expressing Cre‐driven AAV9 GFP with a magnified view.

Article Snippet: We injected C57Bl/6 mice with AAV9 CamKII‐ArchT‐GFP (#99039; Addgene, 120 nl) in the right VL and allowed 2 weeks for expression.

Techniques: Expressing