Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

Techniques:

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

Techniques: Expressing

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

Techniques: Western Blot, Molecular Weight

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

Techniques:

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: Primers used for the real time PCR.

Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

Techniques:

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

Techniques:

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

Techniques: Expressing

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

Techniques: Western Blot, Molecular Weight

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

Techniques:

Journal: PLoS ONE

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

doi: 10.1371/journal.pone.0026334

Figure Lengend Snippet: Primers used for the real time PCR.

Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

Techniques:

Journal: Metabolites

Article Title: Loss of Brain Angiogenesis Inhibitor-3 (BAI3) G-Protein Coupled Receptor in Mice Regulates Adaptive Thermogenesis by Enhancing Energy Expenditure

doi: 10.3390/metabo13060711

Figure Lengend Snippet: QPCR primer list.

Article Snippet: Elov3 anti-goat (sc-54879) and Dio2 anti-rabbit (sc-98716) antibodies were from Santa Cruz Biotechnology.

Techniques: