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    Bio-Techne corporation goat igg isotype control
    Characterisation of TAM receptor expression in a panel of human LMS cell lines. ( A ) Lysates from IB112, IB118, IB133, IB134, IB136, SK-LMS-1 and HepG2 (positive <t>control)</t> were analysed by western blot. Beta-actin was used as loading control. Longer exposition is shown for HepG2 with AXL antibody. ( B ) Leiomyosarcoma cells were starved then stimulated with GAS6. After normalisation for protein concentration, phosphotyrosine proteins were immunoprecipitated with a PY20 antibody. The SK-LMS-1 lysate was immunoprecipitated also with an antibody <t>isotype</t> control. Western blot analysis was performed with an <t>anti</t> TYRO3 antibody. ( C ) Graph showing AXL (red), MER (blue) and TYRO3 (green) expression levels in LMS and HepG2 cells by FACS analysis. Results are shown as fold increase compared to isotype control. ( D ) Evaluation of GAS6 levels in LMS cells lysates by ELISA.
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    Bio-Techne corporation α cbx8
    Characterisation of TAM receptor expression in a panel of human LMS cell lines. ( A ) Lysates from IB112, IB118, IB133, IB134, IB136, SK-LMS-1 and HepG2 (positive <t>control)</t> were analysed by western blot. Beta-actin was used as loading control. Longer exposition is shown for HepG2 with AXL antibody. ( B ) Leiomyosarcoma cells were starved then stimulated with GAS6. After normalisation for protein concentration, phosphotyrosine proteins were immunoprecipitated with a PY20 antibody. The SK-LMS-1 lysate was immunoprecipitated also with an antibody <t>isotype</t> control. Western blot analysis was performed with an <t>anti</t> TYRO3 antibody. ( C ) Graph showing AXL (red), MER (blue) and TYRO3 (green) expression levels in LMS and HepG2 cells by FACS analysis. Results are shown as fold increase compared to isotype control. ( D ) Evaluation of GAS6 levels in LMS cells lysates by ELISA.
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    Image Search Results


    Characterisation of TAM receptor expression in a panel of human LMS cell lines. ( A ) Lysates from IB112, IB118, IB133, IB134, IB136, SK-LMS-1 and HepG2 (positive control) were analysed by western blot. Beta-actin was used as loading control. Longer exposition is shown for HepG2 with AXL antibody. ( B ) Leiomyosarcoma cells were starved then stimulated with GAS6. After normalisation for protein concentration, phosphotyrosine proteins were immunoprecipitated with a PY20 antibody. The SK-LMS-1 lysate was immunoprecipitated also with an antibody isotype control. Western blot analysis was performed with an anti TYRO3 antibody. ( C ) Graph showing AXL (red), MER (blue) and TYRO3 (green) expression levels in LMS and HepG2 cells by FACS analysis. Results are shown as fold increase compared to isotype control. ( D ) Evaluation of GAS6 levels in LMS cells lysates by ELISA.

    Journal: British Journal of Cancer

    Article Title: Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma

    doi: 10.1038/bjc.2017.354

    Figure Lengend Snippet: Characterisation of TAM receptor expression in a panel of human LMS cell lines. ( A ) Lysates from IB112, IB118, IB133, IB134, IB136, SK-LMS-1 and HepG2 (positive control) were analysed by western blot. Beta-actin was used as loading control. Longer exposition is shown for HepG2 with AXL antibody. ( B ) Leiomyosarcoma cells were starved then stimulated with GAS6. After normalisation for protein concentration, phosphotyrosine proteins were immunoprecipitated with a PY20 antibody. The SK-LMS-1 lysate was immunoprecipitated also with an antibody isotype control. Western blot analysis was performed with an anti TYRO3 antibody. ( C ) Graph showing AXL (red), MER (blue) and TYRO3 (green) expression levels in LMS and HepG2 cells by FACS analysis. Results are shown as fold increase compared to isotype control. ( D ) Evaluation of GAS6 levels in LMS cells lysates by ELISA.

    Article Snippet: All cells were then stained with the following goat polyclonal primary antibodies: anti-TYRO3 (AF859); anti-AXL (AF154); anti-Mer (AF891) all from R&D Systems; or goat IgG isotype control (Novus Biologicals, Cambridge, UK), and a secondary donkey anti-goat IgG Alexa Fluor 488 antibody (Invitrogen).

    Techniques: Expressing, Positive Control, Western Blot, Protein Concentration, Immunoprecipitation, Enzyme-linked Immunosorbent Assay

    Crizotinib and foretinib deactivate TYRO3 and AXL phosphorylation and lead to decrease in cell viability. ( A ) Proteins with phosphorylated tyrosines were immunoprecipitated with a PY20 antibody from LMS cell lysates treated with crizotinib and foretinib. IB118 and SK-LMS-1 were precipitated also with an isotype control antibody, as shown in the right part of the panel. Western blot analysis was performed with an anti-TYRO3 and -AXL antibodies. Graphs showing TYRO3 ( B ) or AXL ( C ) protein quantification of western blot using ChemiDoc Imaging Systems (Bio-Rad). Leiomyosarcoma cells were treated with crizotinib ( D ) and foretinib ( E ) at indicated concentrations for 72 h. Viable cells were measured using CellTiterGlo (Promega) and plotted relative to untreated control. Graphs represent means of three independent experiments performed in triplicates. Bars represent s.d.’s.

    Journal: British Journal of Cancer

    Article Title: Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma

    doi: 10.1038/bjc.2017.354

    Figure Lengend Snippet: Crizotinib and foretinib deactivate TYRO3 and AXL phosphorylation and lead to decrease in cell viability. ( A ) Proteins with phosphorylated tyrosines were immunoprecipitated with a PY20 antibody from LMS cell lysates treated with crizotinib and foretinib. IB118 and SK-LMS-1 were precipitated also with an isotype control antibody, as shown in the right part of the panel. Western blot analysis was performed with an anti-TYRO3 and -AXL antibodies. Graphs showing TYRO3 ( B ) or AXL ( C ) protein quantification of western blot using ChemiDoc Imaging Systems (Bio-Rad). Leiomyosarcoma cells were treated with crizotinib ( D ) and foretinib ( E ) at indicated concentrations for 72 h. Viable cells were measured using CellTiterGlo (Promega) and plotted relative to untreated control. Graphs represent means of three independent experiments performed in triplicates. Bars represent s.d.’s.

    Article Snippet: All cells were then stained with the following goat polyclonal primary antibodies: anti-TYRO3 (AF859); anti-AXL (AF154); anti-Mer (AF891) all from R&D Systems; or goat IgG isotype control (Novus Biologicals, Cambridge, UK), and a secondary donkey anti-goat IgG Alexa Fluor 488 antibody (Invitrogen).

    Techniques: Immunoprecipitation, Western Blot, Imaging