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    Addgene inc ria h2b yfp plasmid
    ( A ) Schematic diagram of the approach: Replication Incompetent Avian <t>(RIA)</t> retrovirus encoding nuclear <t>H2B-YFP</t> was injected into the lumen of the hindbrain from which cardiac neural crest arises. ( B ) One day post-infection (HH14), whole mount image (lateral view) showing virally labeled progeny (green) in the cardiac migration stream en route to pharyngeal arch 3. ( B’ ) Brightfield image to show anatomical information. A, anterior; P, posterior; D, dorsal; V, ventral. ( C ) Two days post-infection (HH18), virally labeled cardiac crest has populated pharyngeal arches 3, 4 and 6, highlighted with dashed line. ( D ) Transverse section showing that labeled cardiac crest expresses neural crest marker HNK-1 (red). D, dorsal; V, ventral; L, left; R, right. ( E ) Low magnification transverse section of an E6 embryo (DAPI, blue). Dashed boxes show relative positions of cardiac crest-derived populations. ( F–H ) High magnification image of selected regions in E: pharyngeal arch arteries ( F ); aorticopulmonary septum ( G ); Neural crest derivatives located in the outflow tract express Troponin T (magenta), a myocardium marker ( H, H’ ). ( I ) Temporal map of the establishment of distinct cardiac neural crest derivatives. Labeled cells initially are in the migration stream, cranial nerve IX (CN-IX) and mesenchyme around pharyngeal arch arteries (PAA). Subsequently, they populate the aorticopulmonary septum (APS), myocardium (MYO) and interventricular septum (IVS). Separate channels are displayed in . Light blue indicates known neural crest derivatives. Dark blue reflects newly discovered neural crest derivatives. Scale bars: B, C, E 400 μm; D, F, G, H 100 μm.
    Ria H2b Yfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ria h2b yfp plasmid/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ria h2b yfp plasmid - by Bioz Stars, 2023-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Schematic diagram of the approach: Replication Incompetent Avian (RIA) retrovirus encoding nuclear H2B-YFP was injected into the lumen of the hindbrain from which cardiac neural crest arises. ( B ) One day post-infection (HH14), whole mount image (lateral view) showing virally labeled progeny (green) in the cardiac migration stream en route to pharyngeal arch 3. ( B’ ) Brightfield image to show anatomical information. A, anterior; P, posterior; D, dorsal; V, ventral. ( C ) Two days post-infection (HH18), virally labeled cardiac crest has populated pharyngeal arches 3, 4 and 6, highlighted with dashed line. ( D ) Transverse section showing that labeled cardiac crest expresses neural crest marker HNK-1 (red). D, dorsal; V, ventral; L, left; R, right. ( E ) Low magnification transverse section of an E6 embryo (DAPI, blue). Dashed boxes show relative positions of cardiac crest-derived populations. ( F–H ) High magnification image of selected regions in E: pharyngeal arch arteries ( F ); aorticopulmonary septum ( G ); Neural crest derivatives located in the outflow tract express Troponin T (magenta), a myocardium marker ( H, H’ ). ( I ) Temporal map of the establishment of distinct cardiac neural crest derivatives. Labeled cells initially are in the migration stream, cranial nerve IX (CN-IX) and mesenchyme around pharyngeal arch arteries (PAA). Subsequently, they populate the aorticopulmonary septum (APS), myocardium (MYO) and interventricular septum (IVS). Separate channels are displayed in . Light blue indicates known neural crest derivatives. Dark blue reflects newly discovered neural crest derivatives. Scale bars: B, C, E 400 μm; D, F, G, H 100 μm.

    Journal: eLife

    Article Title: Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish

    doi: 10.7554/eLife.47929

    Figure Lengend Snippet: ( A ) Schematic diagram of the approach: Replication Incompetent Avian (RIA) retrovirus encoding nuclear H2B-YFP was injected into the lumen of the hindbrain from which cardiac neural crest arises. ( B ) One day post-infection (HH14), whole mount image (lateral view) showing virally labeled progeny (green) in the cardiac migration stream en route to pharyngeal arch 3. ( B’ ) Brightfield image to show anatomical information. A, anterior; P, posterior; D, dorsal; V, ventral. ( C ) Two days post-infection (HH18), virally labeled cardiac crest has populated pharyngeal arches 3, 4 and 6, highlighted with dashed line. ( D ) Transverse section showing that labeled cardiac crest expresses neural crest marker HNK-1 (red). D, dorsal; V, ventral; L, left; R, right. ( E ) Low magnification transverse section of an E6 embryo (DAPI, blue). Dashed boxes show relative positions of cardiac crest-derived populations. ( F–H ) High magnification image of selected regions in E: pharyngeal arch arteries ( F ); aorticopulmonary septum ( G ); Neural crest derivatives located in the outflow tract express Troponin T (magenta), a myocardium marker ( H, H’ ). ( I ) Temporal map of the establishment of distinct cardiac neural crest derivatives. Labeled cells initially are in the migration stream, cranial nerve IX (CN-IX) and mesenchyme around pharyngeal arch arteries (PAA). Subsequently, they populate the aorticopulmonary septum (APS), myocardium (MYO) and interventricular septum (IVS). Separate channels are displayed in . Light blue indicates known neural crest derivatives. Dark blue reflects newly discovered neural crest derivatives. Scale bars: B, C, E 400 μm; D, F, G, H 100 μm.

    Article Snippet: Using a standard transfection protocol, chick DF1 cells (ATCC, Manassas, VA; #CRL-12203, Lot number 62712171, RRID: CVCL_0570 , Certificate of Analysis with negative mycoplasma testing at the ATCC website) were transfected with RIA-H2B-YFP plasmid (RRID: Addgene_96893 ) and ENV-A plasmid in 15 cm culture dishes.

    Techniques: Injection, Infection, Labeling, Migration, Marker, Derivative Assay

    ( A ) Separate channels of . All H2B-YFP labeled cells that migrate out of the neural tube are HNK1-positive cardiac neural crest cells (H2B-YFP, green; HNK1, red; DAPI, blue). ( B ) Separate channels of . H2B-YFP labeled cardiac neural crest is present in myocardium of the outflow tract (H2B-YFP, green; Troponin T, magenta; DAPI, blue).

    Journal: eLife

    Article Title: Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish

    doi: 10.7554/eLife.47929

    Figure Lengend Snippet: ( A ) Separate channels of . All H2B-YFP labeled cells that migrate out of the neural tube are HNK1-positive cardiac neural crest cells (H2B-YFP, green; HNK1, red; DAPI, blue). ( B ) Separate channels of . H2B-YFP labeled cardiac neural crest is present in myocardium of the outflow tract (H2B-YFP, green; Troponin T, magenta; DAPI, blue).

    Article Snippet: Using a standard transfection protocol, chick DF1 cells (ATCC, Manassas, VA; #CRL-12203, Lot number 62712171, RRID: CVCL_0570 , Certificate of Analysis with negative mycoplasma testing at the ATCC website) were transfected with RIA-H2B-YFP plasmid (RRID: Addgene_96893 ) and ENV-A plasmid in 15 cm culture dishes.

    Techniques: Labeling

    ( A, B ) Retrovirally labeled cardiac crest cells (H2B-YFP, green) that migrate into the outflow tract ( A , OFT) and pharyngeal arch arteries ( B ) express smooth muscle actin (SMA, magenta) marker. ( C, D ) Labeled cardiac crest cells that enter the ventricle express myocardial marker Troponin T (magenta) ( C ), and myocardial terminal differentiation marker Myosin Heavy Chain (MHC, magenta) ( D ) enclosed in dashed line. ( E, F ) Neural crest-derived cardiomyocytes are not actively dividing or undergoing apoptosis, as demonstrated by phosphohistone H3 staining (PH3, magenta) ( E ) and Caspase 3 staining (magenta) ( F ). Transverse view of E6 embryos. Separate channels are displayed in supplement 1. Scale bars: 100 μm.

    Journal: eLife

    Article Title: Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish

    doi: 10.7554/eLife.47929

    Figure Lengend Snippet: ( A, B ) Retrovirally labeled cardiac crest cells (H2B-YFP, green) that migrate into the outflow tract ( A , OFT) and pharyngeal arch arteries ( B ) express smooth muscle actin (SMA, magenta) marker. ( C, D ) Labeled cardiac crest cells that enter the ventricle express myocardial marker Troponin T (magenta) ( C ), and myocardial terminal differentiation marker Myosin Heavy Chain (MHC, magenta) ( D ) enclosed in dashed line. ( E, F ) Neural crest-derived cardiomyocytes are not actively dividing or undergoing apoptosis, as demonstrated by phosphohistone H3 staining (PH3, magenta) ( E ) and Caspase 3 staining (magenta) ( F ). Transverse view of E6 embryos. Separate channels are displayed in supplement 1. Scale bars: 100 μm.

    Article Snippet: Using a standard transfection protocol, chick DF1 cells (ATCC, Manassas, VA; #CRL-12203, Lot number 62712171, RRID: CVCL_0570 , Certificate of Analysis with negative mycoplasma testing at the ATCC website) were transfected with RIA-H2B-YFP plasmid (RRID: Addgene_96893 ) and ENV-A plasmid in 15 cm culture dishes.

    Techniques: Labeling, Marker, Derivative Assay, Staining

    ( A ) Separate channels of . H2B-YFP virus labeled cardiac neural crest is present in myocardium of the ventricle and expresses cardiomyocyte marker Troponin T (H2B-YFP, green; Troponin T, magenta; DAPI, blue; enclosed in dashed line). ( B ) Separate channels of . H2B-YFP virus labeled cardiac neural crest is present in myocardium of the ventricle and expresses cardiomyocyte terminal differentiation marker myosin heavy chain (MHC) (H2B-YFP, green; MHC, magenta; DAPI, blue; enclosed in dashed line).

    Journal: eLife

    Article Title: Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish

    doi: 10.7554/eLife.47929

    Figure Lengend Snippet: ( A ) Separate channels of . H2B-YFP virus labeled cardiac neural crest is present in myocardium of the ventricle and expresses cardiomyocyte marker Troponin T (H2B-YFP, green; Troponin T, magenta; DAPI, blue; enclosed in dashed line). ( B ) Separate channels of . H2B-YFP virus labeled cardiac neural crest is present in myocardium of the ventricle and expresses cardiomyocyte terminal differentiation marker myosin heavy chain (MHC) (H2B-YFP, green; MHC, magenta; DAPI, blue; enclosed in dashed line).

    Article Snippet: Using a standard transfection protocol, chick DF1 cells (ATCC, Manassas, VA; #CRL-12203, Lot number 62712171, RRID: CVCL_0570 , Certificate of Analysis with negative mycoplasma testing at the ATCC website) were transfected with RIA-H2B-YFP plasmid (RRID: Addgene_96893 ) and ENV-A plasmid in 15 cm culture dishes.

    Techniques: Labeling, Marker

    Journal: eLife

    Article Title: Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish

    doi: 10.7554/eLife.47929

    Figure Lengend Snippet:

    Article Snippet: Using a standard transfection protocol, chick DF1 cells (ATCC, Manassas, VA; #CRL-12203, Lot number 62712171, RRID: CVCL_0570 , Certificate of Analysis with negative mycoplasma testing at the ATCC website) were transfected with RIA-H2B-YFP plasmid (RRID: Addgene_96893 ) and ENV-A plasmid in 15 cm culture dishes.

    Techniques: Transformation Assay, Recombinant, Software