96-well plates Search Results


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  • 85
    Thermo Fisher 96 well centrisep plates
    96 Well Centrisep Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 5 article reviews
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    96 well centrisep plates - by Bioz Stars, 2020-02
    85/100 stars
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    89
    Bio-Rad ddpcr 96 well plates
    Ddpcr 96 Well Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ddpcr 96 well plates/product/Bio-Rad
    Average 89 stars, based on 14 article reviews
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    ddpcr 96 well plates - by Bioz Stars, 2020-02
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    95
    Corning Life Sciences 96 well plates
    RS reduces OGD/R injury in H9C2 cell. H9C2 cells were seeded in <t>96-well</t> plate at 4 × 10 3 cells/hole. After 24 hours, the cells were treated with different concentrations of RS for 24 hours. RS + OGD/R group was performed as follows: H9C2 cells were incubated by 1 μM RS for 3 hours. Then, the cells were performed by OGD/R and normal circumstance for 21 hours after the cells were washed by PBS. (A, C) MTT assay detected cell viability. (B, E) miR-17-3p level was evaluated by qRT-PCR assays. (D) LDH leakage was assessed by colorimetric assays. (F-H) The levels of LC3II/LC3I and cleaved caspase-3 were measured by western blot. Values were presented by mean ± SD, and the relationship between two groups was analyzed by ANOVA with Tukey's test (* vs. Control group, ^ vs. OGD/R group; **^^ p
    96 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 95/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well plates - by Bioz Stars, 2020-02
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    94
    Greiner Bio 96 well plates
    (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in <t>96-well</t> deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.
    96 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Iwaki America 96 well plates
    (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in <t>96-well</t> deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.
    96 Well Plates, supplied by Iwaki America, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Nest Biotechnology 96 well plates
    (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in <t>96-well</t> deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.
    96 Well Plates, supplied by Nest Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well plates - by Bioz Stars, 2020-02
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    93
    PerkinElmer 96 well plates
    (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in <t>96-well</t> deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.
    96 Well Plates, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well plates - by Bioz Stars, 2020-02
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    93
    Sarstedt 96 well plates
    (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in <t>96-well</t> deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.
    96 Well Plates, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well plates - by Bioz Stars, 2020-02
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    93
    TPP-Techno Plastic 96 well plates
    (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in <t>96-well</t> deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.
    96 Well Plates, supplied by TPP-Techno Plastic, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well plates - by Bioz Stars, 2020-02
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    95
    Becton Dickinson 96 well plates
    EF24 shows more potent cytotoxic effect than curcumin on cancer cells. Cells were grown in <t>96-well</t> plates and were treated with EF24 or curcumin as indicated for 48 h. Cell viability was assessed by the sulforhodamine B method and expressed as percentage
    96 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad 96 well plates
    Confirmation of mutation entry clones by barcoded next-gen sequencing Products of the PCR mutagenesis reaction are cloned into the pDONR223 vector via Gateway BP reaction and transformed into competent DH5α bacterial cells. Transformants are spotted on LB + Spectinomycin plates to isolate single colonies. One plate is shown here as a representation. This scheme illustrates the steps involved in mutation sample preparation and next-generation sequencing confirmation. PCR amplification should be done for each mutant at the single colony level prior to sample preparation (illustrated by 96 well plates M1-M4). If a given gene has multiple mutations to clone ( e.g. , four as shown), these mutation PCR products should be separated into different pools (Steps 35-36). A unique barcode adaptor is added to each pool by ligation (Step 37). All pooled mutation PCR products are then sequence-verified by next-generation sequencing technologies (454-FLX, Illumina Solexa, etc) (Steps 38-41).
    96 Well Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific 96 well plates
    Confirmation of mutation entry clones by barcoded next-gen sequencing Products of the PCR mutagenesis reaction are cloned into the pDONR223 vector via Gateway BP reaction and transformed into competent DH5α bacterial cells. Transformants are spotted on LB + Spectinomycin plates to isolate single colonies. One plate is shown here as a representation. This scheme illustrates the steps involved in mutation sample preparation and next-generation sequencing confirmation. PCR amplification should be done for each mutant at the single colony level prior to sample preparation (illustrated by 96 well plates M1-M4). If a given gene has multiple mutations to clone ( e.g. , four as shown), these mutation PCR products should be separated into different pools (Steps 35-36). A unique barcode adaptor is added to each pool by ligation (Step 37). All pooled mutation PCR products are then sequence-verified by next-generation sequencing technologies (454-FLX, Illumina Solexa, etc) (Steps 38-41).
    96 Well Plates, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    LabTech Inc 96 ­well plates
    Confirmation of mutation entry clones by barcoded next-gen sequencing Products of the PCR mutagenesis reaction are cloned into the pDONR223 vector via Gateway BP reaction and transformed into competent DH5α bacterial cells. Transformants are spotted on LB + Spectinomycin plates to isolate single colonies. One plate is shown here as a representation. This scheme illustrates the steps involved in mutation sample preparation and next-generation sequencing confirmation. PCR amplification should be done for each mutant at the single colony level prior to sample preparation (illustrated by 96 well plates M1-M4). If a given gene has multiple mutations to clone ( e.g. , four as shown), these mutation PCR products should be separated into different pools (Steps 35-36). A unique barcode adaptor is added to each pool by ligation (Step 37). All pooled mutation PCR products are then sequence-verified by next-generation sequencing technologies (454-FLX, Illumina Solexa, etc) (Steps 38-41).
    96 ­Well Plates, supplied by LabTech Inc, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Pharmingen 96 well plates
    Confirmation of mutation entry clones by barcoded next-gen sequencing Products of the PCR mutagenesis reaction are cloned into the pDONR223 vector via Gateway BP reaction and transformed into competent DH5α bacterial cells. Transformants are spotted on LB + Spectinomycin plates to isolate single colonies. One plate is shown here as a representation. This scheme illustrates the steps involved in mutation sample preparation and next-generation sequencing confirmation. PCR amplification should be done for each mutant at the single colony level prior to sample preparation (illustrated by 96 well plates M1-M4). If a given gene has multiple mutations to clone ( e.g. , four as shown), these mutation PCR products should be separated into different pools (Steps 35-36). A unique barcode adaptor is added to each pool by ligation (Step 37). All pooled mutation PCR products are then sequence-verified by next-generation sequencing technologies (454-FLX, Illumina Solexa, etc) (Steps 38-41).
    96 Well Plates, supplied by Pharmingen, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega 96 well plates
    Subcutaneous and visceral adipose-derived mesenchymal stem cells (ASCs) display comparable cell surface marker profiles, cell cycle distribution and cell proliferation. ( A ) Immunofluorescence staining of mesenchymal stem cell surface markers CD90 (green) and CD73 (red), and DNA (DAPI, blue) in subcutaneous ASCs (ASCsub) and visceral ASCs (ASCvis). Scale: 20 μm. ( B ) Flow cytometric analyses of positive cell surface markers CD90, CD73, CD146, and CD105, and negative markers CD14, CD31, CD106, and CD34 for mesenchymal stem cells (MSCs). Values represent the percentages of ASCs expressing the indicated protein. The results from eight independent experiments (donors) are presented as mean ± standard error of the mean (SEM). ( C , D ) Cell cycle distribution was analyzed using a FACSCalibur TM . Profile examples were shown ( C ). Cell cycle phases of ASCs were presented in percentage and the results were derived from four independent experiments ( D ). ( E , F ) ASCs were stained for pHH3 (S10) (green), α-tubulin (yellow), pericentrin (red) and DNA (blue), and representatives are shown ( E ). Scale: 10 μm. pHH3 positive cells were quantified in ASCsub and ASCvis ( F ). The results are from three independent experiments with ASCs from three different donors and presented as median ± min/max whiskers in box plots. n.s. > 0.05. ( G ) Cellular extracts from ASCs were prepared for Western blot analyses with indicated antibodies. β-actin served as loading control. ( H ) ASCs were seeded in <t>96-well</t> plates for 0, 24, 48, 72, and 96 h. Cell viability was measured via CellTiter-Blue ® assay. The results are presented as mean ± SEM and statistically analyzed, showing no significant difference (n.s.).
    96 Well Plates, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    USA Scientific Inc 96 well plates
    Subcutaneous and visceral adipose-derived mesenchymal stem cells (ASCs) display comparable cell surface marker profiles, cell cycle distribution and cell proliferation. ( A ) Immunofluorescence staining of mesenchymal stem cell surface markers CD90 (green) and CD73 (red), and DNA (DAPI, blue) in subcutaneous ASCs (ASCsub) and visceral ASCs (ASCvis). Scale: 20 μm. ( B ) Flow cytometric analyses of positive cell surface markers CD90, CD73, CD146, and CD105, and negative markers CD14, CD31, CD106, and CD34 for mesenchymal stem cells (MSCs). Values represent the percentages of ASCs expressing the indicated protein. The results from eight independent experiments (donors) are presented as mean ± standard error of the mean (SEM). ( C , D ) Cell cycle distribution was analyzed using a FACSCalibur TM . Profile examples were shown ( C ). Cell cycle phases of ASCs were presented in percentage and the results were derived from four independent experiments ( D ). ( E , F ) ASCs were stained for pHH3 (S10) (green), α-tubulin (yellow), pericentrin (red) and DNA (blue), and representatives are shown ( E ). Scale: 10 μm. pHH3 positive cells were quantified in ASCsub and ASCvis ( F ). The results are from three independent experiments with ASCs from three different donors and presented as median ± min/max whiskers in box plots. n.s. > 0.05. ( G ) Cellular extracts from ASCs were prepared for Western blot analyses with indicated antibodies. β-actin served as loading control. ( H ) ASCs were seeded in <t>96-well</t> plates for 0, 24, 48, 72, and 96 h. Cell viability was measured via CellTiter-Blue ® assay. The results are presented as mean ± SEM and statistically analyzed, showing no significant difference (n.s.).
    96 Well Plates, supplied by USA Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well plates - by Bioz Stars, 2020-02
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    93
    ibidi 96 well plates
    Subcutaneous and visceral adipose-derived mesenchymal stem cells (ASCs) display comparable cell surface marker profiles, cell cycle distribution and cell proliferation. ( A ) Immunofluorescence staining of mesenchymal stem cell surface markers CD90 (green) and CD73 (red), and DNA (DAPI, blue) in subcutaneous ASCs (ASCsub) and visceral ASCs (ASCvis). Scale: 20 μm. ( B ) Flow cytometric analyses of positive cell surface markers CD90, CD73, CD146, and CD105, and negative markers CD14, CD31, CD106, and CD34 for mesenchymal stem cells (MSCs). Values represent the percentages of ASCs expressing the indicated protein. The results from eight independent experiments (donors) are presented as mean ± standard error of the mean (SEM). ( C , D ) Cell cycle distribution was analyzed using a FACSCalibur TM . Profile examples were shown ( C ). Cell cycle phases of ASCs were presented in percentage and the results were derived from four independent experiments ( D ). ( E , F ) ASCs were stained for pHH3 (S10) (green), α-tubulin (yellow), pericentrin (red) and DNA (blue), and representatives are shown ( E ). Scale: 10 μm. pHH3 positive cells were quantified in ASCsub and ASCvis ( F ). The results are from three independent experiments with ASCs from three different donors and presented as median ± min/max whiskers in box plots. n.s. > 0.05. ( G ) Cellular extracts from ASCs were prepared for Western blot analyses with indicated antibodies. β-actin served as loading control. ( H ) ASCs were seeded in <t>96-well</t> plates for 0, 24, 48, 72, and 96 h. Cell viability was measured via CellTiter-Blue ® assay. The results are presented as mean ± SEM and statistically analyzed, showing no significant difference (n.s.).
    96 Well Plates, supplied by ibidi, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Miltenyi Biotec 96 well plates
    Modeling of gas delivery through 3 permeable-bottom <t>96-well</t> cell culture plates.
    96 Well Plates, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    R&D Systems 96 well plates
    Modeling of gas delivery through 3 permeable-bottom <t>96-well</t> cell culture plates.
    96 Well Plates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Applied BioPhysics 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beyotime 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Waters Corporation 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    CELLTREAT Scientific 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well plates - by Bioz Stars, 2020-02
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    89
    Dynatech Laboratories 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Dynatech Laboratories, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by MJ Research, used in various techniques. Bioz Stars score: 89/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
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    DYNEX tech 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 89/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
    96 Well Plates, supplied by Innovadyne Technologies, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Axygen 96 well plates
    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in <t>96-well</t> plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P
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    Image Search Results


    RS reduces OGD/R injury in H9C2 cell. H9C2 cells were seeded in 96-well plate at 4 × 10 3 cells/hole. After 24 hours, the cells were treated with different concentrations of RS for 24 hours. RS + OGD/R group was performed as follows: H9C2 cells were incubated by 1 μM RS for 3 hours. Then, the cells were performed by OGD/R and normal circumstance for 21 hours after the cells were washed by PBS. (A, C) MTT assay detected cell viability. (B, E) miR-17-3p level was evaluated by qRT-PCR assays. (D) LDH leakage was assessed by colorimetric assays. (F-H) The levels of LC3II/LC3I and cleaved caspase-3 were measured by western blot. Values were presented by mean ± SD, and the relationship between two groups was analyzed by ANOVA with Tukey's test (* vs. Control group, ^ vs. OGD/R group; **^^ p

    Journal: Cellular Reprogramming

    Article Title: Rosuvastatin Attenuates Myocardial Ischemia-Reperfusion Injury via Upregulating miR-17-3p-Mediated Autophagy

    doi: 10.1089/cell.2018.0053

    Figure Lengend Snippet: RS reduces OGD/R injury in H9C2 cell. H9C2 cells were seeded in 96-well plate at 4 × 10 3 cells/hole. After 24 hours, the cells were treated with different concentrations of RS for 24 hours. RS + OGD/R group was performed as follows: H9C2 cells were incubated by 1 μM RS for 3 hours. Then, the cells were performed by OGD/R and normal circumstance for 21 hours after the cells were washed by PBS. (A, C) MTT assay detected cell viability. (B, E) miR-17-3p level was evaluated by qRT-PCR assays. (D) LDH leakage was assessed by colorimetric assays. (F-H) The levels of LC3II/LC3I and cleaved caspase-3 were measured by western blot. Values were presented by mean ± SD, and the relationship between two groups was analyzed by ANOVA with Tukey's test (* vs. Control group, ^ vs. OGD/R group; **^^ p

    Article Snippet: In brief, the cells were seeded at 96-well plates (Corning) at 4 × 103 cells/hole and incubated for 24 hours.

    Techniques: Incubation, MTT Assay, Quantitative RT-PCR, Western Blot

    OGD/R decreases H9C2 cell viability and increases the expression of miR-17-3p. OGD/R was performed by means of free FBS and no-glucose Dulbecco's modified Eagle's medium (DMEM) in 5% CO 2 , 1% O 2 , and 37°C for 6 hours, then higher-glucose DMEM, 10% FBS and 1% penicillin-streptomycin in 5% CO 2 , 95% air, and 37°C for 1 hour. H9C2 cells were seeded in 96-well plate at 4 × 10 3 cells/hole for 24 hours. Then, H9C2 cells were treated with OGD/R for 24 hours or 48 hours. (A) Cell viability was detected by MTT assay. (B) The expression level of miR-17-3p was analyzed via qRT-PCR assays. Values were presented by mean ± SD, and the relationship between the two groups was analyzed by ANOVA with Tukey's test (* vs. Control group, * p

    Journal: Cellular Reprogramming

    Article Title: Rosuvastatin Attenuates Myocardial Ischemia-Reperfusion Injury via Upregulating miR-17-3p-Mediated Autophagy

    doi: 10.1089/cell.2018.0053

    Figure Lengend Snippet: OGD/R decreases H9C2 cell viability and increases the expression of miR-17-3p. OGD/R was performed by means of free FBS and no-glucose Dulbecco's modified Eagle's medium (DMEM) in 5% CO 2 , 1% O 2 , and 37°C for 6 hours, then higher-glucose DMEM, 10% FBS and 1% penicillin-streptomycin in 5% CO 2 , 95% air, and 37°C for 1 hour. H9C2 cells were seeded in 96-well plate at 4 × 10 3 cells/hole for 24 hours. Then, H9C2 cells were treated with OGD/R for 24 hours or 48 hours. (A) Cell viability was detected by MTT assay. (B) The expression level of miR-17-3p was analyzed via qRT-PCR assays. Values were presented by mean ± SD, and the relationship between the two groups was analyzed by ANOVA with Tukey's test (* vs. Control group, * p

    Article Snippet: In brief, the cells were seeded at 96-well plates (Corning) at 4 × 103 cells/hole and incubated for 24 hours.

    Techniques: Expressing, Modification, MTT Assay, Quantitative RT-PCR

    sh FZD 7  reduced the anoikis resistance and spheroid formation ability of  CH 1 and  OV 17R. Percentage of apoptotic (A)  CH 1‐shLuci,  CH 1‐sh FZD 7 ‐1, and  CH 1‐sh FZD 7 ‐2 and (F)  OV 17R‐shLuci,  OV 17R‐sh FZD 7 ‐1, and  OV 17R‐sh FZD 7 ‐2 cells after 48 h in suspension, as analyzed by flow cytometry (10 000 events). The percentage of Annexin V + /PI −  were considered apoptotic. Values (mean ±  SD ) in red denoted the percentage of apoptotic cells from three independent experiments.  X ‐axis: the levels of Annexin V tagged with pacific blue;  y ‐axis: the levels of  PI . Bar charts showing the caspase 3/7 activities—apoptotic cells ( y ‐axis) for (B)  CH 1‐shLuci (dark red bars),  CH 1‐sh FZD 7 ‐1 (green bars), and  CH 1‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis) and (G)  OV 17R‐shLuci (dark red bars),  OV 17R‐sh FZD 7 ‐1 (green bars), and  OV 17R‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis). Viability assay showing live cells ( y ‐axis) for (C)  CH 1‐shLuci (dark red bars),  CH 1‐sh FZD 7 ‐1(green bars), and  CH 1‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis) and (H)  OV 17R‐shLuci (dark red bars),  OV 17R‐sh FZD 7 ‐1 (green bars), and  OV 17R‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis). Cells were seeded at a density of 10 000 cells per well in flat‐bottom  ULA  96‐well plates for three wells per clone. After 72 h, cell viability was measured by fluorescence reading (400 Ex /505 Em ), and cell death was measure d by luminescence readout. (D)  CH 1‐shLuci,  CH 1‐sh FZD 7 ‐1, and  CH 1‐sh FZD 7 ‐2 cells were seeded at a density of 200 cells per well and (I)  OV 17R‐shLuci,  OV 17R‐sh FZD 7 ‐1, and  OV 17R‐sh FZD 7 ‐2 cells were seeded at a density of 500 cells per well in flat‐bottom  ULA  96‐well plates for 10 wells per clone. After 14 days in culture, phase‐contrast images (left panel), calcein‐ AM  staining (middle panel) for viable cells, and EthD‐1 staining (right panel) for dead cells were analyzed. Scale bars represented 100 μm ( CH 1) and 50 μm ( OV 17R). Bar charts showing numbers of spheroids formed ( y ‐axis) in (E)  CH 1 clones ( x ‐axis) and (J)  OV 17R clones after 7 days in suspension. Only spheroids with a diameter greater than 50 μm were counted. Error bars indicated  SEM . Unpaired  t ‐tests were performed for statistical significance.

    Journal: Molecular Oncology

    Article Title: The FZD7‐ TWIST1 axis is responsible for anoikis resistance and tumorigenesis in ovarian carcinoma

    doi: 10.1002/1878-0261.12425

    Figure Lengend Snippet: sh FZD 7 reduced the anoikis resistance and spheroid formation ability of CH 1 and OV 17R. Percentage of apoptotic (A) CH 1‐shLuci, CH 1‐sh FZD 7 ‐1, and CH 1‐sh FZD 7 ‐2 and (F) OV 17R‐shLuci, OV 17R‐sh FZD 7 ‐1, and OV 17R‐sh FZD 7 ‐2 cells after 48 h in suspension, as analyzed by flow cytometry (10 000 events). The percentage of Annexin V + /PI − were considered apoptotic. Values (mean ±  SD ) in red denoted the percentage of apoptotic cells from three independent experiments. X ‐axis: the levels of Annexin V tagged with pacific blue; y ‐axis: the levels of PI . Bar charts showing the caspase 3/7 activities—apoptotic cells ( y ‐axis) for (B) CH 1‐shLuci (dark red bars), CH 1‐sh FZD 7 ‐1 (green bars), and CH 1‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis) and (G) OV 17R‐shLuci (dark red bars), OV 17R‐sh FZD 7 ‐1 (green bars), and OV 17R‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis). Viability assay showing live cells ( y ‐axis) for (C) CH 1‐shLuci (dark red bars), CH 1‐sh FZD 7 ‐1(green bars), and CH 1‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis) and (H) OV 17R‐shLuci (dark red bars), OV 17R‐sh FZD 7 ‐1 (green bars), and OV 17R‐sh FZD 7 ‐2 (orange bars) cells ( x ‐axis). Cells were seeded at a density of 10 000 cells per well in flat‐bottom ULA 96‐well plates for three wells per clone. After 72 h, cell viability was measured by fluorescence reading (400 Ex /505 Em ), and cell death was measure d by luminescence readout. (D) CH 1‐shLuci, CH 1‐sh FZD 7 ‐1, and CH 1‐sh FZD 7 ‐2 cells were seeded at a density of 200 cells per well and (I) OV 17R‐shLuci, OV 17R‐sh FZD 7 ‐1, and OV 17R‐sh FZD 7 ‐2 cells were seeded at a density of 500 cells per well in flat‐bottom ULA 96‐well plates for 10 wells per clone. After 14 days in culture, phase‐contrast images (left panel), calcein‐ AM staining (middle panel) for viable cells, and EthD‐1 staining (right panel) for dead cells were analyzed. Scale bars represented 100 μm ( CH 1) and 50 μm ( OV 17R). Bar charts showing numbers of spheroids formed ( y ‐axis) in (E) CH 1 clones ( x ‐axis) and (J) OV 17R clones after 7 days in suspension. Only spheroids with a diameter greater than 50 μm were counted. Error bars indicated SEM . Unpaired t ‐tests were performed for statistical significance.

    Article Snippet: For the caspase 3/7 activity assay, cells were seeded in ULA 96‐well plates (#7007; Corning) at a density of 10 000 (OV17R, OVCA429, and OV7 clones) or 5000 (CH1 clones) cells per well.

    Techniques: Flow Cytometry, Cytometry, Viability Assay, Fluorescence, Staining, Ethidium Homodimer Assay, Clone Assay

    TWIST 1  rescued the anoikis resistance in sh FZD 7  clones. Percentage of apoptotic (A)  CH 1‐sh FZD 7 ‐1 and  TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 and (G)  OV 17R‐sh FZD 7 ‐1 and  TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 after 72 h in suspension, as analyzed by  FACS  (10 000 events). The percentage of Annexin V + / PI −  were considered apoptotic. Values (mean ±  SD ) in red denote the percentage of apoptotic cells from three independent experiments.  X ‐axis: the levels of Annexin V tagged with pacific blue;  y ‐axis: the levels of  PI . Western blots for E‐cadherin, vimentin,  TWIST 1, and Actin in (B)  CH 1‐sh FZD 7 ‐1 and  TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 clones and in (H)  OV 17R‐sh FZD 7 ‐1 and  TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 clones. (C)  CH 1‐sh FZD 7 ‐1,  TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 cells were seeded at a density of 200 cells per well and (I)  OV 17R‐sh FZD 7 ‐1,  TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 cells were seeded at a density of 500 cells per well in flat‐bottom  ULA  96‐well plates for 10 wells per clone. After 10 days, phase‐contrast images, calcein‐ AM  staining for viable cells, and EthD‐1 staining for dead cells were analyzed. Spheroid quantification (D) shows number of spheroids formed per 2000 cells. Scale bars represented 100 μm. Bar charts showing the caspase 3/7 activities—apoptotic cells ( y ‐axis) for (E)  CH 1‐sh FZD 7 ‐1 (green bars),  TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 (dark red bars;  x ‐axis) and (J)  OV 17R‐sh FZD 7 ‐1 (green bars),  TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 (dark red bars;  x ‐axis). Viability assay showing live cells ( y ‐axis) for (F)  CH 1‐sh FZD 7 ‐1 (green bars),  TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 (dark red bars;  x ‐axis) and (K)  OV 17R‐sh FZD 7 ‐1 (green bars),  TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 (dark red bars;  x ‐axis). Error bars indicated  SEM . Unpaired  t ‐tests were performed for statistical significance.

    Journal: Molecular Oncology

    Article Title: The FZD7‐ TWIST1 axis is responsible for anoikis resistance and tumorigenesis in ovarian carcinoma

    doi: 10.1002/1878-0261.12425

    Figure Lengend Snippet: TWIST 1 rescued the anoikis resistance in sh FZD 7 clones. Percentage of apoptotic (A) CH 1‐sh FZD 7 ‐1 and TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 and (G) OV 17R‐sh FZD 7 ‐1 and TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 after 72 h in suspension, as analyzed by FACS (10 000 events). The percentage of Annexin V + / PI − were considered apoptotic. Values (mean ±  SD ) in red denote the percentage of apoptotic cells from three independent experiments. X ‐axis: the levels of Annexin V tagged with pacific blue; y ‐axis: the levels of PI . Western blots for E‐cadherin, vimentin, TWIST 1, and Actin in (B) CH 1‐sh FZD 7 ‐1 and TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 clones and in (H) OV 17R‐sh FZD 7 ‐1 and TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 clones. (C) CH 1‐sh FZD 7 ‐1, TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 cells were seeded at a density of 200 cells per well and (I) OV 17R‐sh FZD 7 ‐1, TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 cells were seeded at a density of 500 cells per well in flat‐bottom ULA 96‐well plates for 10 wells per clone. After 10 days, phase‐contrast images, calcein‐ AM staining for viable cells, and EthD‐1 staining for dead cells were analyzed. Spheroid quantification (D) shows number of spheroids formed per 2000 cells. Scale bars represented 100 μm. Bar charts showing the caspase 3/7 activities—apoptotic cells ( y ‐axis) for (E) CH 1‐sh FZD 7 ‐1 (green bars), TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 (dark red bars; x ‐axis) and (J) OV 17R‐sh FZD 7 ‐1 (green bars), TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 (dark red bars; x ‐axis). Viability assay showing live cells ( y ‐axis) for (F) CH 1‐sh FZD 7 ‐1 (green bars), TWIST 1 ‐ CH 1‐sh FZD 7 ‐1 (dark red bars; x ‐axis) and (K) OV 17R‐sh FZD 7 ‐1 (green bars), TWIST 1 ‐ OV 17R‐sh FZD 7 ‐1 (dark red bars; x ‐axis). Error bars indicated SEM . Unpaired t ‐tests were performed for statistical significance.

    Article Snippet: For the caspase 3/7 activity assay, cells were seeded in ULA 96‐well plates (#7007; Corning) at a density of 10 000 (OV17R, OVCA429, and OV7 clones) or 5000 (CH1 clones) cells per well.

    Techniques: Clone Assay, FACS, Western Blot, Staining, Ethidium Homodimer Assay, Viability Assay

    PORCN  inhibitor C59 treatment in  CH 1 and  OV 17R clones. (A)  CH 1‐shLuci,  CH 1‐sh FZD 7 ‐1 cells were seeded at a density of 200 cells per well and (B)  OV 17R‐shLuci,  OV 17R‐sh FZD 7 ‐1 cells were seeded at a density of 500 cells per well in flat‐bottom  ULA  96‐well plates for 10 wells per clone. After 10 days in culture with  DMSO  or C59 treatment, phase‐contrast images, calcein‐ AM  staining for viable cells, and EthD‐1 staining for dead cells were analyzed. Scale bars represented 200 μm. Bar charts showing (C) the numbers and (D) the surface areas of spheroids formed by  CH 1‐shLuci and  OV 17R‐shLuci with  DMSO  (dark red bars) or C59 (green bars) treatment for 10 days in suspension. Only spheroids with a diameter greater than 50 μm were counted. Bar charts showing caspase 3/7 activities ( y ‐axis) of  DMSO  (dark red bars) or C59 (green bars) treated (E)  CH 1‐Luci,  CH 1‐sh FZD 7 ‐1, and (F)  OV 17R‐Luci,  OV 17R‐sh FZD 7 ‐1 clones. Cells were seeded at a density of 10 000 cells per well in flat‐bottom  ULA  96‐well plates for three wells per clone. After 72 h, cell viability was measured by fluorescence reading (400 Ex /505 Em ) and cell death was measured by luminescence readout. (G) Bar charts showing the fold change of  TWIST 1  (left) and  BCL 2  (right)  mRNA  expression (2 −∆Ct ;  y ‐axis) in  CH 1‐shLuci and  OV 17R‐shLuci with  DMSO  (dark red bars) or C59 (green bars) treatment for 3 days.  mRNA  expression levels were measured by  qPCR  normalized with a panel of housekeeping genes,  ACTB ,  B2M , GAPDH , RPL 13A , and  HPRT 1 . (H) Bar chart showing percentage of Annexin V‐positive cells in  CH 1‐shLuci,  CH 1‐sh FZD 7 ‐1, (I)  OV 17R‐shLuci and  OV 17R sh FZD 7 ‐1 after treating the cells with  DMSO  control (dark red bars) and C59 (green bars). Values (mean ±  SD ) for Annexin V‐positive cells denoted the percentage of apoptotic cells from three independent experiments. Error bars indicated  SEM . Unpaired  t ‐tests were performed for statistical significance. C59 was used to treat cells at a final concentration of 10 n m . Unpaired  t ‐tests were performed for statistical significance.

    Journal: Molecular Oncology

    Article Title: The FZD7‐ TWIST1 axis is responsible for anoikis resistance and tumorigenesis in ovarian carcinoma

    doi: 10.1002/1878-0261.12425

    Figure Lengend Snippet: PORCN inhibitor C59 treatment in CH 1 and OV 17R clones. (A) CH 1‐shLuci, CH 1‐sh FZD 7 ‐1 cells were seeded at a density of 200 cells per well and (B) OV 17R‐shLuci, OV 17R‐sh FZD 7 ‐1 cells were seeded at a density of 500 cells per well in flat‐bottom ULA 96‐well plates for 10 wells per clone. After 10 days in culture with DMSO or C59 treatment, phase‐contrast images, calcein‐ AM staining for viable cells, and EthD‐1 staining for dead cells were analyzed. Scale bars represented 200 μm. Bar charts showing (C) the numbers and (D) the surface areas of spheroids formed by CH 1‐shLuci and OV 17R‐shLuci with DMSO (dark red bars) or C59 (green bars) treatment for 10 days in suspension. Only spheroids with a diameter greater than 50 μm were counted. Bar charts showing caspase 3/7 activities ( y ‐axis) of DMSO (dark red bars) or C59 (green bars) treated (E) CH 1‐Luci, CH 1‐sh FZD 7 ‐1, and (F) OV 17R‐Luci, OV 17R‐sh FZD 7 ‐1 clones. Cells were seeded at a density of 10 000 cells per well in flat‐bottom ULA 96‐well plates for three wells per clone. After 72 h, cell viability was measured by fluorescence reading (400 Ex /505 Em ) and cell death was measured by luminescence readout. (G) Bar charts showing the fold change of TWIST 1 (left) and BCL 2 (right) mRNA expression (2 −∆Ct ; y ‐axis) in CH 1‐shLuci and OV 17R‐shLuci with DMSO (dark red bars) or C59 (green bars) treatment for 3 days. mRNA expression levels were measured by qPCR normalized with a panel of housekeeping genes, ACTB , B2M , GAPDH , RPL 13A , and HPRT 1 . (H) Bar chart showing percentage of Annexin V‐positive cells in CH 1‐shLuci, CH 1‐sh FZD 7 ‐1, (I) OV 17R‐shLuci and OV 17R sh FZD 7 ‐1 after treating the cells with DMSO control (dark red bars) and C59 (green bars). Values (mean ±  SD ) for Annexin V‐positive cells denoted the percentage of apoptotic cells from three independent experiments. Error bars indicated SEM . Unpaired t ‐tests were performed for statistical significance. C59 was used to treat cells at a final concentration of 10 n m . Unpaired t ‐tests were performed for statistical significance.

    Article Snippet: For the caspase 3/7 activity assay, cells were seeded in ULA 96‐well plates (#7007; Corning) at a density of 10 000 (OV17R, OVCA429, and OV7 clones) or 5000 (CH1 clones) cells per well.

    Techniques: Staining, Ethidium Homodimer Assay, Clone Assay, Fluorescence, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay

    TWIST 1  played a crucial role in anoikis resistance in  OVCA 429 and  OV 7. Percentage of apoptotic (A)  EV ‐ OVCA 429 and  TWIST 1 ‐ tGFP ‐ OVCA 429 and (E)  OV 7‐shLuci,  OV 7‐sh TWIST 1 ‐1, and  OV 7‐sh TWIST 1 ‐2 (G) after 48 h in suspension, as analyzed by  FACS  (10 000 events). The percentage of Annexin V + / PI −  were considered apoptotic. Values (mean ±  SD ) in red denote the percentage of apoptotic cells from three independent experiments.  X ‐axis: the levels of Annexin V tagged with pacific blue;  y ‐axis: the levels of  PI . (B) Western blots for E‐cadherin, vimentin,  TWIST 1 , and  GAPDH  in parental  OVCA 429,  TWIST 1 ‐ tGFP  transfected  OVCA 429 before  FACS  sorting, as well as negative  GFP , intermediate  GFP , and high  GFP  subgroup  OVCA 429 after sorting. (C)  EV ‐ and  TWIST 1 ‐ tGFP ‐ OVCA 429 cells were seeded at a density of 500 cells per well and (G)  OV 7 shLuci, sh TWIST 1 ‐1, and sh TWIST 1 ‐2 cells were seeded at a density of 200 cells per well in flat‐bottom  ULA  96‐well plates for 10 wells per clone. After 14 days in culture, phase‐contrast images, calcein‐ AM  staining for viable cells, and EthD‐1 staining for dead cells were analyzed. Scale bars represented 100 μm. Bar charts showing numbers of spheroids formed by (D)  OVCA 429 clones  TWIST 1  tGFP  (green bars) or by (H)  OV 7 clones shLuci (dark red bars), sh TWIST 1 ‐1 (green bars) and sh TWIST 1 ‐2 (orange bars) after 14 days in suspension. Only spheroids with a diameter more than 50 μm were counted. Error bars indicated  SEM . Unpaired  t ‐tests were performed for statistical significance. (F) Western blots for E‐cadherin, vimentin,  TWIST 1, and  GAPDH  in  OV 7 shLuci, sh TWIST 1 ‐1, and sh TWIST 1 ‐2 clones.

    Journal: Molecular Oncology

    Article Title: The FZD7‐ TWIST1 axis is responsible for anoikis resistance and tumorigenesis in ovarian carcinoma

    doi: 10.1002/1878-0261.12425

    Figure Lengend Snippet: TWIST 1 played a crucial role in anoikis resistance in OVCA 429 and OV 7. Percentage of apoptotic (A) EV ‐ OVCA 429 and TWIST 1 ‐ tGFP ‐ OVCA 429 and (E) OV 7‐shLuci, OV 7‐sh TWIST 1 ‐1, and OV 7‐sh TWIST 1 ‐2 (G) after 48 h in suspension, as analyzed by FACS (10 000 events). The percentage of Annexin V + / PI − were considered apoptotic. Values (mean ±  SD ) in red denote the percentage of apoptotic cells from three independent experiments. X ‐axis: the levels of Annexin V tagged with pacific blue; y ‐axis: the levels of PI . (B) Western blots for E‐cadherin, vimentin, TWIST 1 , and GAPDH in parental OVCA 429, TWIST 1 ‐ tGFP transfected OVCA 429 before FACS sorting, as well as negative GFP , intermediate GFP , and high GFP subgroup OVCA 429 after sorting. (C) EV ‐ and TWIST 1 ‐ tGFP ‐ OVCA 429 cells were seeded at a density of 500 cells per well and (G) OV 7 shLuci, sh TWIST 1 ‐1, and sh TWIST 1 ‐2 cells were seeded at a density of 200 cells per well in flat‐bottom ULA 96‐well plates for 10 wells per clone. After 14 days in culture, phase‐contrast images, calcein‐ AM staining for viable cells, and EthD‐1 staining for dead cells were analyzed. Scale bars represented 100 μm. Bar charts showing numbers of spheroids formed by (D) OVCA 429 clones TWIST 1 tGFP (green bars) or by (H) OV 7 clones shLuci (dark red bars), sh TWIST 1 ‐1 (green bars) and sh TWIST 1 ‐2 (orange bars) after 14 days in suspension. Only spheroids with a diameter more than 50 μm were counted. Error bars indicated SEM . Unpaired t ‐tests were performed for statistical significance. (F) Western blots for E‐cadherin, vimentin, TWIST 1, and GAPDH in OV 7 shLuci, sh TWIST 1 ‐1, and sh TWIST 1 ‐2 clones.

    Article Snippet: For the caspase 3/7 activity assay, cells were seeded in ULA 96‐well plates (#7007; Corning) at a density of 10 000 (OV17R, OVCA429, and OV7 clones) or 5000 (CH1 clones) cells per well.

    Techniques: FACS, Western Blot, Transfection, Staining, Ethidium Homodimer Assay, Clone Assay

    (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in 96-well deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.

    Journal: Frontiers in Microbiology

    Article Title: Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability

    doi: 10.3389/fmicb.2019.02494

    Figure Lengend Snippet: (A,B) Growth of Δ pedE (blue squares) and Δ pedE Δ pvdD (light blue diamonds) in 1 mL liquid M9 medium in 96-well deep-well plates without TES on 5 mM 2-phenylethanol and various concentrations of FeSO 4 in the presence of 10 nM La 3+ (A) or 10 μM La 3+ (B) . OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. (C) Pyoverdine production by strains Δ pedE (left) and Δ pedE Δ pvdD (right) grown on cetrimide agar plates examined under blue light.

    Article Snippet: For luminescence measurements, 198 μl of cell suspension was added to 2 μl of a 100-fold-concentrated metal salt solution in white 96-well plates with a clear bottom (μClear; Greiner Bio-One).

    Techniques: Incubation

    (A) Growth of Δ pedE in 1 mL liquid M9 medium in 96-well deep-well plates on 5 mM 2-phenylethanol and 10 nM La 3+ in the presence of TES or individual components thereof. (B) Growth of Δ pedE as described in (A) with and without the additional supplementation of 50 μM Na 3 -citrate. OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. ∗ Tested condition with 137 nM NaMoO 4 also contains 5 μM H 3 BO 3 and 84 nM NiSO 4 .

    Journal: Frontiers in Microbiology

    Article Title: Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability

    doi: 10.3389/fmicb.2019.02494

    Figure Lengend Snippet: (A) Growth of Δ pedE in 1 mL liquid M9 medium in 96-well deep-well plates on 5 mM 2-phenylethanol and 10 nM La 3+ in the presence of TES or individual components thereof. (B) Growth of Δ pedE as described in (A) with and without the additional supplementation of 50 μM Na 3 -citrate. OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations. ∗ Tested condition with 137 nM NaMoO 4 also contains 5 μM H 3 BO 3 and 84 nM NiSO 4 .

    Article Snippet: For luminescence measurements, 198 μl of cell suspension was added to 2 μl of a 100-fold-concentrated metal salt solution in white 96-well plates with a clear bottom (μClear; Greiner Bio-One).

    Techniques: Incubation

    Growth of strain Δ pedE ( A , dots) and Δ pedH ( B , squares) in 1 mL liquid M9 medium in 96-well deep-well plates on 5 mM 2-phenylethanol and various concentrations of La 3+ in the presence (orange) or absence (blue) of trace element solution (TES). OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations.

    Journal: Frontiers in Microbiology

    Article Title: Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability

    doi: 10.3389/fmicb.2019.02494

    Figure Lengend Snippet: Growth of strain Δ pedE ( A , dots) and Δ pedH ( B , squares) in 1 mL liquid M9 medium in 96-well deep-well plates on 5 mM 2-phenylethanol and various concentrations of La 3+ in the presence (orange) or absence (blue) of trace element solution (TES). OD 600 was determined upon 48 h of incubation at 30°C and 350 rpm. Data are presented as the mean values of biological triplicates and error bars represent the corresponding standard deviations.

    Article Snippet: For luminescence measurements, 198 μl of cell suspension was added to 2 μl of a 100-fold-concentrated metal salt solution in white 96-well plates with a clear bottom (μClear; Greiner Bio-One).

    Techniques: Incubation

    EF24 shows more potent cytotoxic effect than curcumin on cancer cells. Cells were grown in 96-well plates and were treated with EF24 or curcumin as indicated for 48 h. Cell viability was assessed by the sulforhodamine B method and expressed as percentage

    Journal:

    Article Title: Inhibition of IκB Kinase-Nuclear Factor-κB Signaling Pathway by 3,5-Bis(2-flurobenzylidene)piperidin-4-one (EF24), a Novel Monoketone Analog of Curcumin *

    doi: 10.1124/mol.108.046201

    Figure Lengend Snippet: EF24 shows more potent cytotoxic effect than curcumin on cancer cells. Cells were grown in 96-well plates and were treated with EF24 or curcumin as indicated for 48 h. Cell viability was assessed by the sulforhodamine B method and expressed as percentage

    Article Snippet: A549 cells were plated in 96-well plates (BD Biosciences Discovery Labware, Bedford, MA) at 10,000 cells/90 μ l/well and grown for 20 h. Test compounds were added to each well and incubated at 37°C.

    Techniques:

    EF24 impairs TNF- α induced NF- κ B nuclear translocation. A549 cells were grown in 96-well plates and treated with TNF- α or control (DMSO) for 30 min before sample processing for the detection of NF- κ B as described under

    Journal:

    Article Title: Inhibition of IκB Kinase-Nuclear Factor-κB Signaling Pathway by 3,5-Bis(2-flurobenzylidene)piperidin-4-one (EF24), a Novel Monoketone Analog of Curcumin *

    doi: 10.1124/mol.108.046201

    Figure Lengend Snippet: EF24 impairs TNF- α induced NF- κ B nuclear translocation. A549 cells were grown in 96-well plates and treated with TNF- α or control (DMSO) for 30 min before sample processing for the detection of NF- κ B as described under

    Article Snippet: A549 cells were plated in 96-well plates (BD Biosciences Discovery Labware, Bedford, MA) at 10,000 cells/90 μ l/well and grown for 20 h. Test compounds were added to each well and incubated at 37°C.

    Techniques: Translocation Assay

    Confirmation of mutation entry clones by barcoded next-gen sequencing Products of the PCR mutagenesis reaction are cloned into the pDONR223 vector via Gateway BP reaction and transformed into competent DH5α bacterial cells. Transformants are spotted on LB + Spectinomycin plates to isolate single colonies. One plate is shown here as a representation. This scheme illustrates the steps involved in mutation sample preparation and next-generation sequencing confirmation. PCR amplification should be done for each mutant at the single colony level prior to sample preparation (illustrated by 96 well plates M1-M4). If a given gene has multiple mutations to clone ( e.g. , four as shown), these mutation PCR products should be separated into different pools (Steps 35-36). A unique barcode adaptor is added to each pool by ligation (Step 37). All pooled mutation PCR products are then sequence-verified by next-generation sequencing technologies (454-FLX, Illumina Solexa, etc) (Steps 38-41).

    Journal: Nature protocols

    Article Title: Base-resolution stratification of cancer mutations using functional variomics

    doi: 10.1038/nprot.2017.086

    Figure Lengend Snippet: Confirmation of mutation entry clones by barcoded next-gen sequencing Products of the PCR mutagenesis reaction are cloned into the pDONR223 vector via Gateway BP reaction and transformed into competent DH5α bacterial cells. Transformants are spotted on LB + Spectinomycin plates to isolate single colonies. One plate is shown here as a representation. This scheme illustrates the steps involved in mutation sample preparation and next-generation sequencing confirmation. PCR amplification should be done for each mutant at the single colony level prior to sample preparation (illustrated by 96 well plates M1-M4). If a given gene has multiple mutations to clone ( e.g. , four as shown), these mutation PCR products should be separated into different pools (Steps 35-36). A unique barcode adaptor is added to each pool by ligation (Step 37). All pooled mutation PCR products are then sequence-verified by next-generation sequencing technologies (454-FLX, Illumina Solexa, etc) (Steps 38-41).

    Article Snippet: Round-bottom 96-well plates (Corning, cat. no. 3788) 50 ml sterile reagent troughs (Corning, cat. no. 4871) Thin-walled 96-well PCR microtiter plates (Bio-Rad, cat. no. HSP-9601) 15 cm sterile petri dishes (Fisher Scientific, cat. no. 08-757-14) Aluminum foil microplate seal (Bio-Rad, cat. no. MSF-1001) 50 ml polypropylene tubes (BD Falcon, cat. no. 352070) Tissue culture 96 wells flat bottom plate (BD Falcon, cat. no. 353075) PIPETMAN P10, 1 to 10 µl (Gibson, cat. no. F144802) PIPETMAN P200, 50 to 200 µl (Gibson, cat. no. F123601) Corning™ 4084 P10 multichannel pipettes 1 - 10 µl (Thermofisher scientific, cat. no. 07-764-710) Corning™ 4085 P50 multichannel pipettes 5 - 50 µl (Thermofisher scientific, cat. no. 07-764-711) Corning™ 4085 P200 multichannel pipettes 20 - 200 µl (Thermofisher scientific, cat. no. 07-764-712) Corning® Stripettor™ Ultra Pipet Controller (Thermofisher scientific, cat. no. 4099) Thermocycler capable of handling 96-well plates (Bio-Rad, cat. no. T100™) Temperature controlled incubator (30°C) (Thermofisher scientific, cat. no. 15-103-0515) Temperature controlled water bath (42°C) (Thermofisher scientific, cat. no. FSGPD10) 1% E-Gel® 96 Agarose Gels (Thermofisher scientific, cat. no. G700801) E-gel Mother E-Base capable of running 96 samples (Thermofisher scientific, cat. no. EBM03) Temperature controlled shaker for 250 ml flasks and 50 ml tubes (MaxQ™ 6000 Shaker, Thermofisher scientific, cat. no. SHKE6000) Benchtop Centrifuge suitable for microplates and 50 ml tubes (Sorvall™ Legend™ XT/XF centrifuge, Thermofisher scientific, cat. no. 75-004-505) Plate shaker (Mixmate, Eppendorf, cat. no. 022674200) GENESYS™ 10S UV-Vis Spectrophotometer (Thermofisher scientific, cat. no. 840-208100) Vortexer (Thermofisher scientific, cat. no. 88880017TS) Centro XS3 Luminescence Microplate Reader (Berthold Technologies, cat. no. 46970) 1300 Series A2 Class II, Type A2 Bio Safety Cabinets (Thermofisher scientific, cat. no. 13-261-221) Optional: liquid handling robot with 96-well multichannel head and individual well cherry-picking capability (Tecan, cat. no. Fluent 780) Optional: BioRobot Universal System Robot with 96-well DNA miniprep capability (Qiagen, cat. no. 9001094)

    Techniques: Mutagenesis, Clone Assay, Sequencing, Polymerase Chain Reaction, Plasmid Preparation, Transformation Assay, Sample Prep, Next-Generation Sequencing, Amplification, Ligation

    Subcutaneous and visceral adipose-derived mesenchymal stem cells (ASCs) display comparable cell surface marker profiles, cell cycle distribution and cell proliferation. ( A ) Immunofluorescence staining of mesenchymal stem cell surface markers CD90 (green) and CD73 (red), and DNA (DAPI, blue) in subcutaneous ASCs (ASCsub) and visceral ASCs (ASCvis). Scale: 20 μm. ( B ) Flow cytometric analyses of positive cell surface markers CD90, CD73, CD146, and CD105, and negative markers CD14, CD31, CD106, and CD34 for mesenchymal stem cells (MSCs). Values represent the percentages of ASCs expressing the indicated protein. The results from eight independent experiments (donors) are presented as mean ± standard error of the mean (SEM). ( C , D ) Cell cycle distribution was analyzed using a FACSCalibur TM . Profile examples were shown ( C ). Cell cycle phases of ASCs were presented in percentage and the results were derived from four independent experiments ( D ). ( E , F ) ASCs were stained for pHH3 (S10) (green), α-tubulin (yellow), pericentrin (red) and DNA (blue), and representatives are shown ( E ). Scale: 10 μm. pHH3 positive cells were quantified in ASCsub and ASCvis ( F ). The results are from three independent experiments with ASCs from three different donors and presented as median ± min/max whiskers in box plots. n.s. > 0.05. ( G ) Cellular extracts from ASCs were prepared for Western blot analyses with indicated antibodies. β-actin served as loading control. ( H ) ASCs were seeded in 96-well plates for 0, 24, 48, 72, and 96 h. Cell viability was measured via CellTiter-Blue ® assay. The results are presented as mean ± SEM and statistically analyzed, showing no significant difference (n.s.).

    Journal: Cells

    Article Title: Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity

    doi: 10.3390/cells8101288

    Figure Lengend Snippet: Subcutaneous and visceral adipose-derived mesenchymal stem cells (ASCs) display comparable cell surface marker profiles, cell cycle distribution and cell proliferation. ( A ) Immunofluorescence staining of mesenchymal stem cell surface markers CD90 (green) and CD73 (red), and DNA (DAPI, blue) in subcutaneous ASCs (ASCsub) and visceral ASCs (ASCvis). Scale: 20 μm. ( B ) Flow cytometric analyses of positive cell surface markers CD90, CD73, CD146, and CD105, and negative markers CD14, CD31, CD106, and CD34 for mesenchymal stem cells (MSCs). Values represent the percentages of ASCs expressing the indicated protein. The results from eight independent experiments (donors) are presented as mean ± standard error of the mean (SEM). ( C , D ) Cell cycle distribution was analyzed using a FACSCalibur TM . Profile examples were shown ( C ). Cell cycle phases of ASCs were presented in percentage and the results were derived from four independent experiments ( D ). ( E , F ) ASCs were stained for pHH3 (S10) (green), α-tubulin (yellow), pericentrin (red) and DNA (blue), and representatives are shown ( E ). Scale: 10 μm. pHH3 positive cells were quantified in ASCsub and ASCvis ( F ). The results are from three independent experiments with ASCs from three different donors and presented as median ± min/max whiskers in box plots. n.s. > 0.05. ( G ) Cellular extracts from ASCs were prepared for Western blot analyses with indicated antibodies. β-actin served as loading control. ( H ) ASCs were seeded in 96-well plates for 0, 24, 48, 72, and 96 h. Cell viability was measured via CellTiter-Blue ® assay. The results are presented as mean ± SEM and statistically analyzed, showing no significant difference (n.s.).

    Article Snippet: Cell proliferation assays were carried out by using Cell Titer-Blue® Cell Viability Assay (BD Biosciences, Heidelberg, Germany) on treated cells in 96-well plates (Promega, Mannheim, Germany).

    Techniques: Derivative Assay, Marker, Immunofluorescence, Staining, Flow Cytometry, Expressing, Western Blot, CtB Assay

    Modeling of gas delivery through 3 permeable-bottom 96-well cell culture plates.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate

    doi: 10.1152/ajpcell.00276.2018

    Figure Lengend Snippet: Modeling of gas delivery through 3 permeable-bottom 96-well cell culture plates.

    Article Snippet: The 96-well plates available from Miltenyi Biotec allow the interwell volume to be filled with warmed media that also acts as a heat sink to reduce thermal gradients.

    Techniques: Cell Culture

    Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in 96-well plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P

    Journal: Comparative Medicine

    Article Title: Relationships between Cytokine Levels and Disease Parameters during the Development of a Collagen-induced Arthritis Model in Cynomolgus Macaques (Macaca fascicularis)

    doi: 10.30802/AALAS-CM-18-000058

    Figure Lengend Snippet: Proliferation of PBMC from macaques with collagen-induced arthritis (CIA). PBMC (2 × 10 5 cells in 100 μL per well) were seeded in 96-well plates. Cells were cultured with or without CII (40 μg/mL) for 72 h, after which they were evaluated in a BrdU assay. The stimulation index value (mean optical density values of CII-stimulated cultures divided by the mean optical density values of medium-only cultures) was calculated for each treatment. (A) Proliferation of PBMC. (B) Proliferation of PBMC against CII. (C) Stimulation index. Data are expressed as box-and-whisker plots ( n = 3 or 4 per group); group means were compared by using Mann–Whitney U tests. *, Value significantly ( P

    Article Snippet: PBMC (2 × 105 cells/100 μL per well) were seeded in 96-well plates (Thermo Scientific, Waltham, MA).

    Techniques: Cell Culture, BrdU Staining, Whisker Assay, MANN-WHITNEY