96 well polystyrene microtiter plates Search Results


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  • 99
    Millipore untreated polystyrene 96 well microtiter plate
    Untreated Polystyrene 96 Well Microtiter Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher sterile 96 well polystyrene microtiter plates
    Sterile 96 Well Polystyrene Microtiter Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Eppendorf AG 96 well polystyrene microtiter plates
    96 Well Polystyrene Microtiter Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Greiner Bio 96 well polystyrene microtiter plate
    96 Well Polystyrene Microtiter Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Corning Life Sciences 96 well microtiter polystyrene plates
    Biofilm formation by S. mutans UA159 (wild type), TJ- relA , and JL-Δ relA . Cultures were grown in a <t>96-well</t> <t>microtiter</t> plate containing BM at 37°C in 5% CO 2 for 48 h. Growth (dotted bars) and biofilm formation (solid bars) were measured at absorbances of 600 and 575 nm, respectively. (A) The graph shows the averages and standard deviations for three independent experiments. (B) Crystal violet-stained biofilms of strains UA159 (lane 1), TJ- relA (lane 2), and JL-Δ relA (lane 3) on polystyrene plates. Typical results for three independent experiments are presented.
    96 Well Microtiter Polystyrene Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sumitomo Dainippon 96 well polystyrene microtiter plates
    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated <t>96-well</t> <t>microtiter</t> plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p
    96 Well Polystyrene Microtiter Plates, supplied by Sumitomo Dainippon, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Orange Scientific 96 well polystyrene microtiter plates
    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated <t>96-well</t> <t>microtiter</t> plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p
    96 Well Polystyrene Microtiter Plates, supplied by Orange Scientific, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Iwaki America 96 well polystyrene microtiter plates
    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated <t>96-well</t> <t>microtiter</t> plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p
    96 Well Polystyrene Microtiter Plates, supplied by Iwaki America, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 96 well polystyrene microtiter plates
    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated <t>96-well</t> <t>microtiter</t> plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p
    96 Well Polystyrene Microtiter Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DYNEX tech 96 well polystyrene microtiter plates
    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated <t>96-well</t> <t>microtiter</t> plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p
    96 Well Polystyrene Microtiter Plates, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sarstedt 96 well microtiter polystyrene plate
    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated <t>96-well</t> <t>microtiter</t> plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p
    96 Well Microtiter Polystyrene Plate, supplied by Sarstedt, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad 96 well microtiter polystyrene plates
    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated <t>96-well</t> <t>microtiter</t> plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p
    96 Well Microtiter Polystyrene Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher 96 well polystyrene microtiter plate
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    96 Well Polystyrene Microtiter Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific 96 well polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    96 Well Polystyrene Microtiter Plates, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 96 well polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    96 Well Polystyrene Microtiter Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher black 96 well polystyrene microtiter plate
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    Black 96 Well Polystyrene Microtiter Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Iwaki America sterile 96 well polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    Sterile 96 Well Polystyrene Microtiter Plates, supplied by Iwaki America, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Corning Life Sciences 96 well black polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    96 Well Black Polystyrene Microtiter Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Kisker Biotech sterile 96 well polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    Sterile 96 Well Polystyrene Microtiter Plates, supplied by Kisker Biotech, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tpp 96 well polystyrene microtiter plate
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    Tpp 96 Well Polystyrene Microtiter Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Sarstedt sterile 96 well polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
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    Dynatech Laboratories coating polystyrene 96 well microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
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    Becton Dickinson sterile 96 well polystyrene microtiter plate
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
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    Greiner Bio uncoated 96 well polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
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    Orange Scientific sterile 96 wells polystyrene microtiter plate
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    Sterile 96 Wells Polystyrene Microtiter Plate, supplied by Orange Scientific, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences sterile 96 well polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    Sterile 96 Well Polystyrene Microtiter Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences 96 well uncoated polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    96 Well Uncoated Polystyrene Microtiter Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Greiner Bio black polystyrene 96 well microtiter plate
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
    Black Polystyrene 96 Well Microtiter Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dynatech Laboratories 96 well u bottomed polystyrene microtiter plate
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
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    Porvair Sciences 96 well white polystyrene microtiter plates
    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in <t>96-well</t> <t>microtiter</t> plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.
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    Image Search Results


    Biofilm formation by S. mutans UA159 (wild type), TJ- relA , and JL-Δ relA . Cultures were grown in a 96-well microtiter plate containing BM at 37°C in 5% CO 2 for 48 h. Growth (dotted bars) and biofilm formation (solid bars) were measured at absorbances of 600 and 575 nm, respectively. (A) The graph shows the averages and standard deviations for three independent experiments. (B) Crystal violet-stained biofilms of strains UA159 (lane 1), TJ- relA (lane 2), and JL-Δ relA (lane 3) on polystyrene plates. Typical results for three independent experiments are presented.

    Journal: Infection and Immunity

    Article Title: Effects of RelA on Key Virulence Properties of Planktonic and Biofilm Populations of Streptococcus mutans

    doi: 10.1128/IAI.72.3.1431-1440.2004

    Figure Lengend Snippet: Biofilm formation by S. mutans UA159 (wild type), TJ- relA , and JL-Δ relA . Cultures were grown in a 96-well microtiter plate containing BM at 37°C in 5% CO 2 for 48 h. Growth (dotted bars) and biofilm formation (solid bars) were measured at absorbances of 600 and 575 nm, respectively. (A) The graph shows the averages and standard deviations for three independent experiments. (B) Crystal violet-stained biofilms of strains UA159 (lane 1), TJ- relA (lane 2), and JL-Δ relA (lane 3) on polystyrene plates. Typical results for three independent experiments are presented.

    Article Snippet: The ability of cells to form stable biofilms was assessed by growing the cells in biofilm medium (BM) ( ) broth in 96-well polystyrene microtiter plates (Costar 3595; Corning, Inc., Corning, N.Y.) for 24 or 48 h. For scanning electron microscopy (SEM), biofilms were grown in BM on the surface of hydroxylapatite (HA) disks that were deposited in the wells of a 24-well polystyrene tissue culture plate (Falcon 3047; Becton Dickinson, Lincoln Park, N.J.).

    Techniques: Staining

    The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated 96-well microtiter plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p

    Journal: PLoS ONE

    Article Title: The inhibitory effects of polypyrrole on the biofilm formation of Streptococcus mutans

    doi: 10.1371/journal.pone.0225584

    Figure Lengend Snippet: The inhibitory effects of polypyrrole on the biofilm formation of S . mutans . Effects of various concentrations of polypyrrole on the biofilm formation of S . mutans UA159 were observed on human saliva–coated 96-well microtiter plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S . mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S . mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S . mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S . mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S . sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p

    Article Snippet: Biofilm formation Biofilms formation was developed in 96-well polystyrene microtiter plates (Sumitomo Bakelite, Tokyo, Japan), which were previously coated with human saliva, BSA, anti-PAc serum [ ], anti-PAc monoclonal antibody [ ] and anti-GbpC serum [ ] for 1h at 4°C.

    Techniques: Incubation

    The effects of hyaluronic acid on polypyrrole-dependent biofilm formation. The effects of hyaluronic acid on the polypyrrole-dependent biofilm formation of S . mutans were observed. A: Biofilm formation by gtfBC mutant was quantitatively assessed in various concentrations of polypyrrole on human saliva-coated 96-well microtiter plates in TSB supplemented with 0.25% sucrose and various concentrations of polypyrrole. B: Polypyrrole was preincubated with hyaluronic acid and applied in the biofilm formation assay with gtfBC mutant. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a statistically significant difference among multiple groups (ANOVA with Bonferroni correction; * p-values

    Journal: PLoS ONE

    Article Title: The inhibitory effects of polypyrrole on the biofilm formation of Streptococcus mutans

    doi: 10.1371/journal.pone.0225584

    Figure Lengend Snippet: The effects of hyaluronic acid on polypyrrole-dependent biofilm formation. The effects of hyaluronic acid on the polypyrrole-dependent biofilm formation of S . mutans were observed. A: Biofilm formation by gtfBC mutant was quantitatively assessed in various concentrations of polypyrrole on human saliva-coated 96-well microtiter plates in TSB supplemented with 0.25% sucrose and various concentrations of polypyrrole. B: Polypyrrole was preincubated with hyaluronic acid and applied in the biofilm formation assay with gtfBC mutant. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a statistically significant difference among multiple groups (ANOVA with Bonferroni correction; * p-values

    Article Snippet: Biofilm formation Biofilms formation was developed in 96-well polystyrene microtiter plates (Sumitomo Bakelite, Tokyo, Japan), which were previously coated with human saliva, BSA, anti-PAc serum [ ], anti-PAc monoclonal antibody [ ] and anti-GbpC serum [ ] for 1h at 4°C.

    Techniques: Mutagenesis, Tube Formation Assay

    Biofilm formation in S . mutans induced by polypyrrole. Effects of polypyrrole on the biofilm formation of S . mutans were observed in the condition without glucan. Biofilm formation in gtfBC mutant was quantitatively assessed in conditions with various concentrations of polypyrrole on human saliva-coated (A), uncoated (B), BSA-coated (C), anti-PAc antiserum-coated (D), K4A (anti-PAc monoclonal antibody)-coated (E) and anti-GbpC antiserum-coated (F) 96-well microtiter plates in TSB supplemented with 0.25% sucrose. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference among multiple groups (ANOVA with Bonferroni correction; p-values

    Journal: PLoS ONE

    Article Title: The inhibitory effects of polypyrrole on the biofilm formation of Streptococcus mutans

    doi: 10.1371/journal.pone.0225584

    Figure Lengend Snippet: Biofilm formation in S . mutans induced by polypyrrole. Effects of polypyrrole on the biofilm formation of S . mutans were observed in the condition without glucan. Biofilm formation in gtfBC mutant was quantitatively assessed in conditions with various concentrations of polypyrrole on human saliva-coated (A), uncoated (B), BSA-coated (C), anti-PAc antiserum-coated (D), K4A (anti-PAc monoclonal antibody)-coated (E) and anti-GbpC antiserum-coated (F) 96-well microtiter plates in TSB supplemented with 0.25% sucrose. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference among multiple groups (ANOVA with Bonferroni correction; p-values

    Article Snippet: Biofilm formation Biofilms formation was developed in 96-well polystyrene microtiter plates (Sumitomo Bakelite, Tokyo, Japan), which were previously coated with human saliva, BSA, anti-PAc serum [ ], anti-PAc monoclonal antibody [ ] and anti-GbpC serum [ ] for 1h at 4°C.

    Techniques: Mutagenesis

    Biofilm formation by S . gordonii and S . mitis induced by polypyrrole. The effects of polypyrrole on the biofilm formation by S . gordonii and S . mitis were observed. Biofilm formation by S . gordonii ATCC 10558 (A and C) and S . mitis ATCC 6249 (B and D) was quantitatively assessed in various concentrations of polypyrrole on uncoated (A and B) and human saliva-coated (C and D) 96-well microtiter plates in TSB supplemented with 0.25% sucrose in various concentrations of polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a statistically significant difference among multiple groups (ANOVA with Bonferroni correction; p-values

    Journal: PLoS ONE

    Article Title: The inhibitory effects of polypyrrole on the biofilm formation of Streptococcus mutans

    doi: 10.1371/journal.pone.0225584

    Figure Lengend Snippet: Biofilm formation by S . gordonii and S . mitis induced by polypyrrole. The effects of polypyrrole on the biofilm formation by S . gordonii and S . mitis were observed. Biofilm formation by S . gordonii ATCC 10558 (A and C) and S . mitis ATCC 6249 (B and D) was quantitatively assessed in various concentrations of polypyrrole on uncoated (A and B) and human saliva-coated (C and D) 96-well microtiter plates in TSB supplemented with 0.25% sucrose in various concentrations of polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a statistically significant difference among multiple groups (ANOVA with Bonferroni correction; p-values

    Article Snippet: Biofilm formation Biofilms formation was developed in 96-well polystyrene microtiter plates (Sumitomo Bakelite, Tokyo, Japan), which were previously coated with human saliva, BSA, anti-PAc serum [ ], anti-PAc monoclonal antibody [ ] and anti-GbpC serum [ ] for 1h at 4°C.

    Techniques:

    Biofilm formation by S . sanguinis induced by polypyrrole. The effects of polypyrrole on the biofilm formation of S . sanguinis were observed. Biofilm formation by S . sanguinis ATCC 10556 was quantitatively assessed with various concentrations of polypyrrole on human saliva-coated (A)-, uncoated (B), and BSA-coated (C) 96-well microtiter plates in TSB supplemented with 0.25% sucrose, including various concentrations of polypyrrole. (D) The growth of S . sanguinis was assessed by measuring the cell colonization numbers on BHI agar plates after 16 h of incubation in TSB supplemented with 0.25% sucrose, including various concentrations of polypyrrole. Small C indicated no polypyrrole as a control. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a statistically significant difference among multiple groups (ANOVA with Bonferroni correction; p-values

    Journal: PLoS ONE

    Article Title: The inhibitory effects of polypyrrole on the biofilm formation of Streptococcus mutans

    doi: 10.1371/journal.pone.0225584

    Figure Lengend Snippet: Biofilm formation by S . sanguinis induced by polypyrrole. The effects of polypyrrole on the biofilm formation of S . sanguinis were observed. Biofilm formation by S . sanguinis ATCC 10556 was quantitatively assessed with various concentrations of polypyrrole on human saliva-coated (A)-, uncoated (B), and BSA-coated (C) 96-well microtiter plates in TSB supplemented with 0.25% sucrose, including various concentrations of polypyrrole. (D) The growth of S . sanguinis was assessed by measuring the cell colonization numbers on BHI agar plates after 16 h of incubation in TSB supplemented with 0.25% sucrose, including various concentrations of polypyrrole. Small C indicated no polypyrrole as a control. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a statistically significant difference among multiple groups (ANOVA with Bonferroni correction; p-values

    Article Snippet: Biofilm formation Biofilms formation was developed in 96-well polystyrene microtiter plates (Sumitomo Bakelite, Tokyo, Japan), which were previously coated with human saliva, BSA, anti-PAc serum [ ], anti-PAc monoclonal antibody [ ] and anti-GbpC serum [ ] for 1h at 4°C.

    Techniques: Incubation

    Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in 96-well microtiter plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.

    Journal: Biochemistry

    Article Title: Control of Integrin αIIbβ3 Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates

    doi: 10.1021/bi9016022

    Figure Lengend Snippet: Effect of soluble fibrinogen on platelet adhesion to the fibrin gel. (A) Fibrin gels were formed in 96-well microtiter plates by mixing 100 μ L of 1.5 mg/mL fibrinogen with 0.15 unit/mL thrombin. After polymerization for 2 h at 37 °C, thrombin was inactivated by adding PPACK (5 mM). The gels were incubated with different concentrations of fibrinogen for 30 min at 37 °C, the solution above the gels were aspirated, and the gels were incubated for another 1 h at 37 °C. Aliquots (100 μ L) of labeled platelets (1×10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion of platelets to intact fibrin clots (in the absence of fibrinogen)was 16.5±1.2%of added cells. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. (B) Scanning electron microscopy of platelets adherent to the naked fibrin gel (upper panel) and to fibrin coated with 1 mg/mL soluble fibrinogen. Fibrin and fibrinogen-treated fibrin gels were prepared as described in Materials and Methods. Two representative platelets adherent to each substrate are shown.

    Article Snippet: Briefly, the wells of 96-well polystyrene microtiter plates (Immulon 4HBX; Thermo-Labsystems, Franklin, MA) were coated with various concentrations of fibrinogen for 3 h at 37 °C and postcoated with 1.0% PVP for 1 h at 37 °C.

    Techniques: Incubation, Labeling, Fluorescence, Electron Microscopy

    Platelet adhesion to immobilized fibrinogen. Left ordinate: The wells of 96-well microtiter plates were coated with different concentrations of fibrinogen (0.1–50 μ g/mL) for 3 h at 37 °C followed by postcoating with 1%PVP. Aliquots (100 μ L) of labeled platelets (1 × 10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion is shown as fluorescence in arbitrary units. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. Maximal platelet adhesion was 17.3 ± 3.5% of added cells. Right ordinate: The wells of microtiter plates were coated with different concentrations of fibrinogen as described above, and mAb 2G5 was added at 1 μ g/mL. After incubation for 1 h at 37 °C, the microtiter plates were washed, and goat anti-mouse IgG conjugated to alkaline phosphatase was added. The binding was detected by reaction with p -nitrophenyl phosphate, measuring the absorbance at 405 nm.

    Journal: Biochemistry

    Article Title: Control of Integrin αIIbβ3 Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates

    doi: 10.1021/bi9016022

    Figure Lengend Snippet: Platelet adhesion to immobilized fibrinogen. Left ordinate: The wells of 96-well microtiter plates were coated with different concentrations of fibrinogen (0.1–50 μ g/mL) for 3 h at 37 °C followed by postcoating with 1%PVP. Aliquots (100 μ L) of labeled platelets (1 × 10 8 /mL) were added to each well. After 50 min incubation at 37 °C, the nonadherent cells were removed by two washes with PBS, and fluorescence was measured. Adhesion is shown as fluorescence in arbitrary units. The data shown are the means ± SE of four experiments with triplicate determinations at each experimental point. Maximal platelet adhesion was 17.3 ± 3.5% of added cells. Right ordinate: The wells of microtiter plates were coated with different concentrations of fibrinogen as described above, and mAb 2G5 was added at 1 μ g/mL. After incubation for 1 h at 37 °C, the microtiter plates were washed, and goat anti-mouse IgG conjugated to alkaline phosphatase was added. The binding was detected by reaction with p -nitrophenyl phosphate, measuring the absorbance at 405 nm.

    Article Snippet: Briefly, the wells of 96-well polystyrene microtiter plates (Immulon 4HBX; Thermo-Labsystems, Franklin, MA) were coated with various concentrations of fibrinogen for 3 h at 37 °C and postcoated with 1.0% PVP for 1 h at 37 °C.

    Techniques: Labeling, Incubation, Fluorescence, Binding Assay

    Adherence of S. pneumoniae to human epithelial cells ( A ) and in vitro growth ( B ). Detroit nasopharyngeal epithelial cell lines were exposed to non-Ec- Sp containing or lacking ICE 1 Sp ST344 and RD 1 Sp ST344, respectively ( A ). Adherence was determined at 30 min and calculated as the proportion of recovered bacteria to the inoculum. Experiments were repeated three times (three times on three different days: Indicated are mean and SD (standard deviation)). Swiss and Thai strains were classic and sporadic non-Ec- Sp , respectively. For the nine adherence experiments, ordinary one-way ANOVA resulted in P = 0.0052. See text for details. MLST, multilocus sequence type. Isolates of the BC3-NT lineage have been recently defined ( Chewapreecha et al. 2014 ). Measurement of planktonic growth was done in 96-well microtiter polystyrene plates and OD450nm was measured on an ELISA plate reader every 30 min for 22 h ( B ). Each experiment included three technical replicates and each experiment was performed three times.

    Journal: Genome Biology and Evolution

    Article Title: Global Phylogenomic Analysis of Nonencapsulated Streptococcus pneumoniae Reveals a Deep-Branching Classic Lineage That Is Distinct from Multiple Sporadic Lineages

    doi: 10.1093/gbe/evu263

    Figure Lengend Snippet: Adherence of S. pneumoniae to human epithelial cells ( A ) and in vitro growth ( B ). Detroit nasopharyngeal epithelial cell lines were exposed to non-Ec- Sp containing or lacking ICE 1 Sp ST344 and RD 1 Sp ST344, respectively ( A ). Adherence was determined at 30 min and calculated as the proportion of recovered bacteria to the inoculum. Experiments were repeated three times (three times on three different days: Indicated are mean and SD (standard deviation)). Swiss and Thai strains were classic and sporadic non-Ec- Sp , respectively. For the nine adherence experiments, ordinary one-way ANOVA resulted in P = 0.0052. See text for details. MLST, multilocus sequence type. Isolates of the BC3-NT lineage have been recently defined ( Chewapreecha et al. 2014 ). Measurement of planktonic growth was done in 96-well microtiter polystyrene plates and OD450nm was measured on an ELISA plate reader every 30 min for 22 h ( B ). Each experiment included three technical replicates and each experiment was performed three times.

    Article Snippet: Adherence to Human Epithelial Cell Line Detroit 562 and Analysis of Planktonic Growth As for measuring planktonic growth, 96-well microtiter polystyrene plates (Thermo Fisher Scientific, Denmark) were used for the isolates Switzerland 4, 11, Thailand 1 and 2, respectively.

    Techniques: In Vitro, Standard Deviation, Sequencing, Enzyme-linked Immunosorbent Assay