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cd70 expression  (ATCC)
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ATCC cd70 expression
In vitro characterization of [ 68 <t>Ga]Ga-NOTA-anti-CD70</t> VHH. A Analysis of CD70 protein expression measured by flow cytometry using in the upper trace anti-CD70 VHH-Hilyte Fluor 488 (107B8) or control VHH-Hilyte Fluor 488 and in the lower trace PE mouse anti-human CD70 or PE mouse IgG3 isotype control. B Relative amount of cell associated activity of [ 68 Ga]Ga-NOTA-anti-CD70 VHH (5 nM, 0.04 µg) on CD70 high and CD70 low cells (1 × 10 6 ), or in the presence of excess unlabeled VHH (50 µg, block). (C) Specific binding of [ 68 Ga]Ga-NOTA-anti-CD70 VHH on Raji cells (5 × 10 5 ). (**** p < 0.0001)
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In vitro characterization of [ 68 Ga]Ga-NOTA-anti-CD70 VHH. A Analysis of CD70 protein expression measured by flow cytometry using in the upper trace anti-CD70 VHH-Hilyte Fluor 488 (107B8) or control VHH-Hilyte Fluor 488 and in the lower trace PE mouse anti-human CD70 or PE mouse IgG3 isotype control. B Relative amount of cell associated activity of [ 68 Ga]Ga-NOTA-anti-CD70 VHH (5 nM, 0.04 µg) on CD70 high and CD70 low cells (1 × 10 6 ), or in the presence of excess unlabeled VHH (50 µg, block). (C) Specific binding of [ 68 Ga]Ga-NOTA-anti-CD70 VHH on Raji cells (5 × 10 5 ). (**** p < 0.0001)

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: In vitro characterization of [ 68 Ga]Ga-NOTA-anti-CD70 VHH. A Analysis of CD70 protein expression measured by flow cytometry using in the upper trace anti-CD70 VHH-Hilyte Fluor 488 (107B8) or control VHH-Hilyte Fluor 488 and in the lower trace PE mouse anti-human CD70 or PE mouse IgG3 isotype control. B Relative amount of cell associated activity of [ 68 Ga]Ga-NOTA-anti-CD70 VHH (5 nM, 0.04 µg) on CD70 high and CD70 low cells (1 × 10 6 ), or in the presence of excess unlabeled VHH (50 µg, block). (C) Specific binding of [ 68 Ga]Ga-NOTA-anti-CD70 VHH on Raji cells (5 × 10 5 ). (**** p < 0.0001)

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: In Vitro, Expressing, Flow Cytometry, Control, Activity Assay, Blocking Assay, Binding Assay

Biodistribution analysis of [ 68 Ga]Ga-NOTA-anti-CD70 VHH at 15, 30, 60 and 90 min post radiotracer injection. (%ID/g = % injected dose/ gram, injected radiotracer dose = 1–4.7 µg; ~ 1.5–2.5 MBq/mouse, n = 3/time point)

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: Biodistribution analysis of [ 68 Ga]Ga-NOTA-anti-CD70 VHH at 15, 30, 60 and 90 min post radiotracer injection. (%ID/g = % injected dose/ gram, injected radiotracer dose = 1–4.7 µg; ~ 1.5–2.5 MBq/mouse, n = 3/time point)

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Injection

PET/CT imaging using [ 68 Ga]Ga-NOTA-anti-CD70 VHH fragment— A Representative coronal µPET/CT images (30–60 min post injection timeframe summed activity, slice thickness 0.78 mm) of mice bearing CD70 high (786-O (left panel) and with block (middle panel)) and CD70 low tumors (NCl-H1975, right panel). B Time-activity curves for radiotracer distribution in the tumors are shown. (%ID/mL = % injected dose/ mL, injected radiotracer dose = 4–6.7 µg; ~ 6.4–7.4 MBq/mouse; n = 7, data are presented as mean ± SEM. **** p < 0.0001; #### p = 0.0029)

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: PET/CT imaging using [ 68 Ga]Ga-NOTA-anti-CD70 VHH fragment— A Representative coronal µPET/CT images (30–60 min post injection timeframe summed activity, slice thickness 0.78 mm) of mice bearing CD70 high (786-O (left panel) and with block (middle panel)) and CD70 low tumors (NCl-H1975, right panel). B Time-activity curves for radiotracer distribution in the tumors are shown. (%ID/mL = % injected dose/ mL, injected radiotracer dose = 4–6.7 µg; ~ 6.4–7.4 MBq/mouse; n = 7, data are presented as mean ± SEM. **** p < 0.0001; #### p = 0.0029)

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Positron Emission Tomography-Computed Tomography, Imaging, Injection, Activity Assay, Blocking Assay

Ex vivo analysis of 786-O and NCl-H1975 tumors. Representative autoradiography (ARG) and microscopy images of adjacent histologic tumor slices stained for CD70 using a biotinylated anti-CD70 VHH

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: Ex vivo analysis of 786-O and NCl-H1975 tumors. Representative autoradiography (ARG) and microscopy images of adjacent histologic tumor slices stained for CD70 using a biotinylated anti-CD70 VHH

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Ex Vivo, Autoradiography, Microscopy, Staining

Binding affinity kinetics (equilibrium dissociation constant (K D ), association (k a ) and dissociation (k d ) rates) of anti-CD70 VHHs as determined by SPR

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: Binding affinity kinetics (equilibrium dissociation constant (K D ), association (k a ) and dissociation (k d ) rates) of anti-CD70 VHHs as determined by SPR

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Binding Assay