954-bp coding sequence Search Results


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  • 99
    Thermo Fisher protein coding regions
    Protein Coding Regions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Genechem ecor i
    Ecor I, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna3 1
    Pcdna3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 49697 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Genechem pcdna3 1
    Notch1 regulates Slug expression by enhancing its promoter activity. (A) and (B) The level of Slug expression was evaluated by western blot and real-time PCR after MDA-MB-231 cells were stably transfected with shNC or shNotch1. (C) and (D) The Slug expression level following treatment with Jagged1 ligand for 48 h was estimated by western blot and real-time PCR. (E) The schematic diagram shows the construction of the pGL3-Slug promoter and its negative control pGL3-basic. (F) Luciferase reporter assays were carried out in MDA-MB-231 cells, which were cotransfected with the pGL3-Slug promoter or its negative control pGL3-basic and N1ICD overexpression plasmid <t>pcDNA3.1(+)</t> or its negative control plasmid pcDNA3.1. Each independent experiment was repeated three times. Column: mean; bar: SD. The symbol * represents a significant difference (P
    Pcdna3 1, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega fopflash luciferase reporter
    Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is <t>FOPflash</t> , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P
    Fopflash Luciferase Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher primer3 software
    Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is <t>FOPflash</t> , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P
    Primer3 Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen gel extraction kit
    Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is <t>FOPflash</t> , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P
    Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 31944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ni nta
    Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is <t>FOPflash</t> , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P
    Ni Nta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc vector pspcas9 bb 2a puro
    Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is <t>FOPflash</t> , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P
    Vector Pspcas9 Bb 2a Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Notch1 regulates Slug expression by enhancing its promoter activity. (A) and (B) The level of Slug expression was evaluated by western blot and real-time PCR after MDA-MB-231 cells were stably transfected with shNC or shNotch1. (C) and (D) The Slug expression level following treatment with Jagged1 ligand for 48 h was estimated by western blot and real-time PCR. (E) The schematic diagram shows the construction of the pGL3-Slug promoter and its negative control pGL3-basic. (F) Luciferase reporter assays were carried out in MDA-MB-231 cells, which were cotransfected with the pGL3-Slug promoter or its negative control pGL3-basic and N1ICD overexpression plasmid pcDNA3.1(+) or its negative control plasmid pcDNA3.1. Each independent experiment was repeated three times. Column: mean; bar: SD. The symbol * represents a significant difference (P

    Journal: Molecular Cancer

    Article Title: Notch1 signaling regulates the epithelial–mesenchymal transition and invasion of breast cancer in a Slug-dependent manner

    doi: 10.1186/s12943-015-0295-3

    Figure Lengend Snippet: Notch1 regulates Slug expression by enhancing its promoter activity. (A) and (B) The level of Slug expression was evaluated by western blot and real-time PCR after MDA-MB-231 cells were stably transfected with shNC or shNotch1. (C) and (D) The Slug expression level following treatment with Jagged1 ligand for 48 h was estimated by western blot and real-time PCR. (E) The schematic diagram shows the construction of the pGL3-Slug promoter and its negative control pGL3-basic. (F) Luciferase reporter assays were carried out in MDA-MB-231 cells, which were cotransfected with the pGL3-Slug promoter or its negative control pGL3-basic and N1ICD overexpression plasmid pcDNA3.1(+) or its negative control plasmid pcDNA3.1. Each independent experiment was repeated three times. Column: mean; bar: SD. The symbol * represents a significant difference (P

    Article Snippet: The 954-bp coding sequence of Slug was amplified by PCR from the cDNAs of MDA-MB-231 cells and subcloned into pcDNA3.1 (+) by EcoR V and EcoR I (Shanghai Genechem Co., Ltd, China).

    Techniques: Expressing, Activity Assay, Western Blot, Real-time Polymerase Chain Reaction, Multiple Displacement Amplification, Stable Transfection, Transfection, Negative Control, Luciferase, Over Expression, Plasmid Preparation

    Slug serves as a mediator for Notch1-induced EMT, migration, and invasion. (A) and (B) Western blot analysis and real-time PCR assays were carried out to evaluate the expression of Slug when MDA-MB-231 cells were transfected with negative control siRNA (siNC) or Slug siRNA (siSlug) for 48 h. (C) MDA-MB-231 cells were transfected with siNC or siSlug for 48 h and then treated with Jagged1 for an additional 48 h. E-cadherin and vimentin protein levels were evaluated by western blot. (D) and (E) MDA-MB-231 cells were transfected with siNC or siSlug for 48 h and then treated with Jagged1 for an additional 48 h. The cells were seeded into a migration chamber or a Matrigel-coated invasion chamber and incubated for 24 h. The number of migrated cells was counted under a light microscope. (F) MDA-MB-231-shNC or MDA-MB-231-shNotch1 cells were transfected with negative control vector (pcDNA3.1) or Slug overexpression vector (pcDNA3.1-Slug), respectively. Forty-eight hours later, western blot analysis was performed to assess the expression levels of E-cadherin and vimentin. (G) and (H) MDA-MB-231-shNotch1 cells were transfected with pcDNA3.1 or pcDNA3.1-Slug for 48 h and then the migration and invasion abilities were evaluated. The data are from three independent experiments. Column: mean; bar: SD. The symbol * represents a significant difference (P

    Journal: Molecular Cancer

    Article Title: Notch1 signaling regulates the epithelial–mesenchymal transition and invasion of breast cancer in a Slug-dependent manner

    doi: 10.1186/s12943-015-0295-3

    Figure Lengend Snippet: Slug serves as a mediator for Notch1-induced EMT, migration, and invasion. (A) and (B) Western blot analysis and real-time PCR assays were carried out to evaluate the expression of Slug when MDA-MB-231 cells were transfected with negative control siRNA (siNC) or Slug siRNA (siSlug) for 48 h. (C) MDA-MB-231 cells were transfected with siNC or siSlug for 48 h and then treated with Jagged1 for an additional 48 h. E-cadherin and vimentin protein levels were evaluated by western blot. (D) and (E) MDA-MB-231 cells were transfected with siNC or siSlug for 48 h and then treated with Jagged1 for an additional 48 h. The cells were seeded into a migration chamber or a Matrigel-coated invasion chamber and incubated for 24 h. The number of migrated cells was counted under a light microscope. (F) MDA-MB-231-shNC or MDA-MB-231-shNotch1 cells were transfected with negative control vector (pcDNA3.1) or Slug overexpression vector (pcDNA3.1-Slug), respectively. Forty-eight hours later, western blot analysis was performed to assess the expression levels of E-cadherin and vimentin. (G) and (H) MDA-MB-231-shNotch1 cells were transfected with pcDNA3.1 or pcDNA3.1-Slug for 48 h and then the migration and invasion abilities were evaluated. The data are from three independent experiments. Column: mean; bar: SD. The symbol * represents a significant difference (P

    Article Snippet: The 954-bp coding sequence of Slug was amplified by PCR from the cDNAs of MDA-MB-231 cells and subcloned into pcDNA3.1 (+) by EcoR V and EcoR I (Shanghai Genechem Co., Ltd, China).

    Techniques: Migration, Western Blot, Real-time Polymerase Chain Reaction, Expressing, Multiple Displacement Amplification, Transfection, Negative Control, Incubation, Light Microscopy, Plasmid Preparation, Over Expression

    Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Opposite Roles of Wnt7a and Sfrp1 in Modulating Proper Development of Neural Progenitors in the Mouse Cerebral Cortex

    doi: 10.3389/fnmol.2018.00247

    Figure Lengend Snippet: Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P

    Article Snippet: The Sfrp1 , Dkk1 coding sequences were subcloned into the pcDNA3.1 vector for the TOPflash and FOPflash luciferase reporter (Promega, United States) assay.

    Techniques: Activity Assay, Luciferase, Reporter Assay, Activation Assay, Binding Assay, Expressing, Plasmid Preparation, Mutagenesis, Transfection