91138 Search Results


s intermedius strain maff 911388  (ATCC)
94
ATCC s intermedius strain maff 911388
S Intermedius Strain Maff 911388, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s intermedius strain maff 911388/product/ATCC
Average 94 stars, based on 1 article reviews
s intermedius strain maff 911388 - by Bioz Stars, 2025-03
94/100 stars
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anti raph1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti raph1
( A ) Map of SNP rs117701653 at <t>RAPH1-CD28-CTLA4-ICOS</t> locus on human chromosome 2q33. ( B ) mRNA levels by low-input RNA sequencing in resting CD4 + T cells from healthy donors (8 A/A, 8 A/C, and 8 C/C genotypes at rs117701653), ( C ) base-edited Jurkat clones (7 A/A, 3 A/C, and 3 C/C), and ( D ) SMCHD1 deleted clones from wild-type or edited clones (4 A/A and 3 C/C). Expression of CTLA-4 was non-detectable in Jurkat cell clones. Western blot detected proteins expression of α-Tubulin (55 KDa), SMCHD1 (225 KDa), ICOS (22 KDa), RAPH1 (195 KDa), CD28 (40-60 KDa). Error bars were expressed as mean ±S.D. For cis-eQTL mapping with the resting CD4 + T cells, we targeted 11 protein coding genes (ICA1L, WDR12, CARF, NBEAL1, CYP20A1, ABI2, RAPH1, CD28, CTLA4, ICOS , and PARD3B ) that have a transcription start site (TSS) within a 1 MB window of SNP rs117701653 ( Supplementary Fig. 2 ). P values were computed using a linear model for association between genotypes and expression levels corrected for age, sex, and rank-normal transformed the residuals ( B ). P values from one-way ANOVA corrected for multiple comparison by FDR using two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli ( C ), or paired t-test with two-tailed significance ( D ) were shown.
Anti Raph1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti raph1/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
anti raph1 - by Bioz Stars, 2025-03
93/100 stars
  Buy from Supplier
pdirect 23a plasmid 91138  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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Image Search Results


( A ) Map of SNP rs117701653 at RAPH1-CD28-CTLA4-ICOS locus on human chromosome 2q33. ( B ) mRNA levels by low-input RNA sequencing in resting CD4 + T cells from healthy donors (8 A/A, 8 A/C, and 8 C/C genotypes at rs117701653), ( C ) base-edited Jurkat clones (7 A/A, 3 A/C, and 3 C/C), and ( D ) SMCHD1 deleted clones from wild-type or edited clones (4 A/A and 3 C/C). Expression of CTLA-4 was non-detectable in Jurkat cell clones. Western blot detected proteins expression of α-Tubulin (55 KDa), SMCHD1 (225 KDa), ICOS (22 KDa), RAPH1 (195 KDa), CD28 (40-60 KDa). Error bars were expressed as mean ±S.D. For cis-eQTL mapping with the resting CD4 + T cells, we targeted 11 protein coding genes (ICA1L, WDR12, CARF, NBEAL1, CYP20A1, ABI2, RAPH1, CD28, CTLA4, ICOS , and PARD3B ) that have a transcription start site (TSS) within a 1 MB window of SNP rs117701653 ( Supplementary Fig. 2 ). P values were computed using a linear model for association between genotypes and expression levels corrected for age, sex, and rank-normal transformed the residuals ( B ). P values from one-way ANOVA corrected for multiple comparison by FDR using two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli ( C ), or paired t-test with two-tailed significance ( D ) were shown.

Journal: bioRxiv

Article Title: Non-coding autoimmune risk variant accelerates T peripheral helper cell development via ICOS

doi: 10.1101/2022.12.16.520733

Figure Lengend Snippet: ( A ) Map of SNP rs117701653 at RAPH1-CD28-CTLA4-ICOS locus on human chromosome 2q33. ( B ) mRNA levels by low-input RNA sequencing in resting CD4 + T cells from healthy donors (8 A/A, 8 A/C, and 8 C/C genotypes at rs117701653), ( C ) base-edited Jurkat clones (7 A/A, 3 A/C, and 3 C/C), and ( D ) SMCHD1 deleted clones from wild-type or edited clones (4 A/A and 3 C/C). Expression of CTLA-4 was non-detectable in Jurkat cell clones. Western blot detected proteins expression of α-Tubulin (55 KDa), SMCHD1 (225 KDa), ICOS (22 KDa), RAPH1 (195 KDa), CD28 (40-60 KDa). Error bars were expressed as mean ±S.D. For cis-eQTL mapping with the resting CD4 + T cells, we targeted 11 protein coding genes (ICA1L, WDR12, CARF, NBEAL1, CYP20A1, ABI2, RAPH1, CD28, CTLA4, ICOS , and PARD3B ) that have a transcription start site (TSS) within a 1 MB window of SNP rs117701653 ( Supplementary Fig. 2 ). P values were computed using a linear model for association between genotypes and expression levels corrected for age, sex, and rank-normal transformed the residuals ( B ). P values from one-way ANOVA corrected for multiple comparison by FDR using two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli ( C ), or paired t-test with two-tailed significance ( D ) were shown.

Article Snippet: Protein extracts were subjected to western blotting using anti-SMCHD1 (ABCAM, ab179456), anti-α-Tubulin (Cellsignal, 2144S), anti-ICOS (ABCAM, ab175401), anti-RAPH1 (Cellsignal, 91138T), anti-CD28 (ABCAM, 38774S) antibodies.

Techniques: RNA Sequencing Assay, Clone Assay, Expressing, Western Blot, Transformation Assay, Two Tailed Test