Article Title: MLK4? functions as a negative regulator of MAPK signaling and cell invasion
Figure Lengend Snippet: MLK4β negatively regulates p38, ERK and JNK signaling. ( a ) HCT116 cells were transfected with MLK3, MLK4 and nonspecific siRNA oligos. Cell lysates were immunoblotted with MLK4β, MLK3, p-p38, p38 and β-Actin antibodies. ( b ) Lysates from HCT116 cells transiently expressing pCMV5-FLAG, FLAG-MLK3 or FLAG-MLK4β were prepared and immunoblotted with FLAG, p-p38 and p38 antibodies. ( c ) HCT116 cells were transfected with nonspecific, MLK3 or MLK4 siRNA, and then treated with 0.5 M sorbitol for 30 min. Cell lysates were immunoblotted with MLK4β, MLK3, p-p38, p38, p-ERK, ERK, p-JNK, JNK and β-Actin antibodies.
Article Snippet: The MLK4 antibody specifically recognizes the C-terminus of MLK4β and does not cross-react with MLK4α (Novus Biologicals, Littleton, CO, USA).
Techniques: Transfection, Expressing