90950 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Novus Biologicals mlk4
    MLK4β suppresses heat- and osmotic stress-induced MAPK signalling SKOV3 cells were transfected with either <t>MLK4</t> or non-specific siRNA and exposed to (A) 250 mM sorbitol for 0, 2, or 4 h, or (B) 42 °C for 0, 1, or 2 h. Whole cell lysates were immunoblotted with the indicated antibodies.
    Mlk4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mlk4/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mlk4 - by Bioz Stars, 2022-11
    90/100 stars
      Buy from Supplier

    Image Search Results


    MLK4β suppresses heat- and osmotic stress-induced MAPK signalling SKOV3 cells were transfected with either MLK4 or non-specific siRNA and exposed to (A) 250 mM sorbitol for 0, 2, or 4 h, or (B) 42 °C for 0, 1, or 2 h. Whole cell lysates were immunoblotted with the indicated antibodies.

    Journal: Cellular signalling

    Article Title: Osmotic and Heat Stress-dependent Regulation of MLK4β and MLK3 by the CHIP E3 Ligase in Ovarian Cancer Cells

    doi: 10.1016/j.cellsig.2017.07.021

    Figure Lengend Snippet: MLK4β suppresses heat- and osmotic stress-induced MAPK signalling SKOV3 cells were transfected with either MLK4 or non-specific siRNA and exposed to (A) 250 mM sorbitol for 0, 2, or 4 h, or (B) 42 °C for 0, 1, or 2 h. Whole cell lysates were immunoblotted with the indicated antibodies.

    Article Snippet: Other antibodies that were used for immunoblotting were GFP and MLK4 (Novus Biologicals), FLAG (Agilent), Ubiquitin (BD Biosciences, San Jose, CA) and Hsp70 (W-27) (Thermo Scientific, Freemont, CA, USA).

    Techniques: Transfection

    MLK4β inhibits MLK3 activity. ( a ) Cell lysates were prepared from HCT116 cells overexpressing pCMV5-FLAG, FLAG-MLK3 or FLAG-MLK4β and immunoblotted with p-MLK3 and FLAG antibodies. ( b ) Cell lysates from SKOV3 cells transiently expressing pCMV5-FLAG or FLAG-MLK4β were immunoblotted with MLK4β, p-MLK3, MLK3, p-p38 and p38 antibodies. ( c ) SKOV3 cells were transfected with nonspecific or MLK4 siRNA and cell lysates were immunoblotted with MLK4β, p-MLK3, MLK3, p-p38, p38 and β-Actin antibodies.

    Journal: Oncogenesis

    Article Title: MLK4? functions as a negative regulator of MAPK signaling and cell invasion

    doi: 10.1038/oncsis.2012.6

    Figure Lengend Snippet: MLK4β inhibits MLK3 activity. ( a ) Cell lysates were prepared from HCT116 cells overexpressing pCMV5-FLAG, FLAG-MLK3 or FLAG-MLK4β and immunoblotted with p-MLK3 and FLAG antibodies. ( b ) Cell lysates from SKOV3 cells transiently expressing pCMV5-FLAG or FLAG-MLK4β were immunoblotted with MLK4β, p-MLK3, MLK3, p-p38 and p38 antibodies. ( c ) SKOV3 cells were transfected with nonspecific or MLK4 siRNA and cell lysates were immunoblotted with MLK4β, p-MLK3, MLK3, p-p38, p38 and β-Actin antibodies.

    Article Snippet: The MLK4 antibody specifically recognizes the C-terminus of MLK4β and does not cross-react with MLK4α (Novus Biologicals, Littleton, CO, USA).

    Techniques: Activity Assay, Expressing, Transfection

    MLK4β negatively regulates p38 and MEK3/MEK6 activation. ( a ) HCT116 cells were transfected with nonspecific or MLK4 siRNA. The cells were left untreated (left panel) or treated (right panel) with 0.5 M sorbitol for 30 min and cell lysates were immunoblotted with MLK4β, p-p38, p38 and β-Actin antibodies. ( b ) HCT116 cells were transiently transfected with pCMV5-FLAG or FLAG-MLK4β and left untreated or treated with 0.5 M sorbitol for 30 min. Cell lysates were immunoblotted with FLAG, p38, p-p38 and actin antibodies. ( c ) HCT116 cells were transfected as described in b and left untreated or treated with 10 μM TNF-α for 20 min. Cell lysates were immunoblotted as described in b . HCT116 ( d ) and HEY1B ( e ) cells were transfected with nonspecific or MLK4 siRNA oligos. Cell lysates were prepared and immunoblotted with MLK4β, p-p38, p38, p-MEK3/MEK6, MEK3/MEK6 and β-Actin antibodies.

    Journal: Oncogenesis

    Article Title: MLK4? functions as a negative regulator of MAPK signaling and cell invasion

    doi: 10.1038/oncsis.2012.6

    Figure Lengend Snippet: MLK4β negatively regulates p38 and MEK3/MEK6 activation. ( a ) HCT116 cells were transfected with nonspecific or MLK4 siRNA. The cells were left untreated (left panel) or treated (right panel) with 0.5 M sorbitol for 30 min and cell lysates were immunoblotted with MLK4β, p-p38, p38 and β-Actin antibodies. ( b ) HCT116 cells were transiently transfected with pCMV5-FLAG or FLAG-MLK4β and left untreated or treated with 0.5 M sorbitol for 30 min. Cell lysates were immunoblotted with FLAG, p38, p-p38 and actin antibodies. ( c ) HCT116 cells were transfected as described in b and left untreated or treated with 10 μM TNF-α for 20 min. Cell lysates were immunoblotted as described in b . HCT116 ( d ) and HEY1B ( e ) cells were transfected with nonspecific or MLK4 siRNA oligos. Cell lysates were prepared and immunoblotted with MLK4β, p-p38, p38, p-MEK3/MEK6, MEK3/MEK6 and β-Actin antibodies.

    Article Snippet: The MLK4 antibody specifically recognizes the C-terminus of MLK4β and does not cross-react with MLK4α (Novus Biologicals, Littleton, CO, USA).

    Techniques: Activation Assay, Transfection

    MLK4β negatively regulates p38, ERK and JNK signaling. ( a ) HCT116 cells were transfected with MLK3, MLK4 and nonspecific siRNA oligos. Cell lysates were immunoblotted with MLK4β, MLK3, p-p38, p38 and β-Actin antibodies. ( b ) Lysates from HCT116 cells transiently expressing pCMV5-FLAG, FLAG-MLK3 or FLAG-MLK4β were prepared and immunoblotted with FLAG, p-p38 and p38 antibodies. ( c ) HCT116 cells were transfected with nonspecific, MLK3 or MLK4 siRNA, and then treated with 0.5 M sorbitol for 30 min. Cell lysates were immunoblotted with MLK4β, MLK3, p-p38, p38, p-ERK, ERK, p-JNK, JNK and β-Actin antibodies.

    Journal: Oncogenesis

    Article Title: MLK4? functions as a negative regulator of MAPK signaling and cell invasion

    doi: 10.1038/oncsis.2012.6

    Figure Lengend Snippet: MLK4β negatively regulates p38, ERK and JNK signaling. ( a ) HCT116 cells were transfected with MLK3, MLK4 and nonspecific siRNA oligos. Cell lysates were immunoblotted with MLK4β, MLK3, p-p38, p38 and β-Actin antibodies. ( b ) Lysates from HCT116 cells transiently expressing pCMV5-FLAG, FLAG-MLK3 or FLAG-MLK4β were prepared and immunoblotted with FLAG, p-p38 and p38 antibodies. ( c ) HCT116 cells were transfected with nonspecific, MLK3 or MLK4 siRNA, and then treated with 0.5 M sorbitol for 30 min. Cell lysates were immunoblotted with MLK4β, MLK3, p-p38, p38, p-ERK, ERK, p-JNK, JNK and β-Actin antibodies.

    Article Snippet: The MLK4 antibody specifically recognizes the C-terminus of MLK4β and does not cross-react with MLK4α (Novus Biologicals, Littleton, CO, USA).

    Techniques: Transfection, Expressing